Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114434 |
| Name | oriT_AR_0157|unnamed2  |
| Organism | Citrobacter sp. CRE-46 strain AR_0157 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP029730 (78837..78941 [-], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 56..61, 74..79 (TGGAAT..ATTCCA) 46..51, 56..61 (ATTCCA..TGGAAT) 1..6, 8..13 (AATTTG..CAAATT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_AR_0157|unnamed2
AATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATT
AATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10723 | GenBank | WP_006472393 |
| Name | t4cp2_AN232_RS28265_AR_0157|unnamed2 |
UniProt ID | _ |
| Length | 667 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 667 a.a. Molecular weight: 72973.03 Da Isoelectric Point: 6.4199
>WP_006472393.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MQGQGVLFGQIAAVLGIVIAGVWGATQWTAAALGYQLRLGSPWFDFFGTPVYHPWRLFEWWFFFDAYAPQ
VFDIGGSIAGGSGLLAVVVAIAMSVWRSRQAKLVTTYGSARWAEADDIRKAGLTQPAGVFLGKYRNEYLR
HEGPEHVLTFAPTRSGKGVGLVVPTLLSWPASAVIHDIKGENWQITAGWRSRFSHCLLFNPTDAKSAAYN
PLLEVRRGAHEVRDVQNIADILVDPEGALEKRNHWEKTSHALLVGAILHVLYAGEDKTLRGVANFLSDPA
SPFELTLHRMMTTKHLGGAPHPVVASAAREVLNKSDNERSGVLSTAMSFLGLYRDPTVAEVTSRCDWRIA
DLIASEHPVSLYLVVPPSDISRTKPLIRLILNQIGRRLTESLDGSDGIERRHKLLLMLDEFPALGRLDFF
ETALAFMAGYGIRSFLIAQSLNQIDKAYGQNHSILDNCHVRVTFATNDERTAKRISETLGTATELRAQRN
YAGHRLAPWLGHLMVSRQETARPLLTPGEVMQLPTDEAVVMVSSVAPIKANKLRYYADANFKSRVLPPPA
LAAGQYADAPPARPDDWSGLAIPAVPTAPATGLSSDDAGTADDGGPRRQPELSEVAEYSPEPQPAGTDLA
LLDDDDDLPLPLPRQFDPAMQRTARLASLDPNDGIDL
MQGQGVLFGQIAAVLGIVIAGVWGATQWTAAALGYQLRLGSPWFDFFGTPVYHPWRLFEWWFFFDAYAPQ
VFDIGGSIAGGSGLLAVVVAIAMSVWRSRQAKLVTTYGSARWAEADDIRKAGLTQPAGVFLGKYRNEYLR
HEGPEHVLTFAPTRSGKGVGLVVPTLLSWPASAVIHDIKGENWQITAGWRSRFSHCLLFNPTDAKSAAYN
PLLEVRRGAHEVRDVQNIADILVDPEGALEKRNHWEKTSHALLVGAILHVLYAGEDKTLRGVANFLSDPA
SPFELTLHRMMTTKHLGGAPHPVVASAAREVLNKSDNERSGVLSTAMSFLGLYRDPTVAEVTSRCDWRIA
DLIASEHPVSLYLVVPPSDISRTKPLIRLILNQIGRRLTESLDGSDGIERRHKLLLMLDEFPALGRLDFF
ETALAFMAGYGIRSFLIAQSLNQIDKAYGQNHSILDNCHVRVTFATNDERTAKRISETLGTATELRAQRN
YAGHRLAPWLGHLMVSRQETARPLLTPGEVMQLPTDEAVVMVSSVAPIKANKLRYYADANFKSRVLPPPA
LAAGQYADAPPARPDDWSGLAIPAVPTAPATGLSSDDAGTADDGGPRRQPELSEVAEYSPEPQPAGTDLA
LLDDDDDLPLPLPRQFDPAMQRTARLASLDPNDGIDL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 55535..65148
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AN232_RS28255 (AN232_28255) | 51635..52555 | + | 921 | Protein_59 | LysR family transcriptional regulator | - |
| AN232_RS32205 (AN232_28260) | 52575..52838 | + | 264 | WP_023106770 | hypothetical protein | - |
| AN232_RS28265 (AN232_28265) | 53074..55077 | + | 2004 | WP_006472393 | conjugal transfer protein TraG | - |
| AN232_RS28270 (AN232_28270) | 55074..55538 | + | 465 | WP_006472392 | ribbon-helix-helix protein, CopG family | - |
| AN232_RS28275 (AN232_28275) | 55535..56602 | + | 1068 | WP_006472391 | P-type conjugative transfer ATPase TrbB | virB11 |
| AN232_RS28280 (AN232_28280) | 56599..56982 | + | 384 | WP_110498890 | TrbC/VirB2 family protein | virB2 |
| AN232_RS28285 (AN232_28285) | 56979..57251 | + | 273 | WP_110498891 | VirB3 family type IV secretion system protein | virB3 |
| AN232_RS28290 (AN232_28290) | 57264..59714 | + | 2451 | WP_110498892 | conjugal transfer protein TrbE | virb4 |
| AN232_RS28295 (AN232_28295) | 59711..60448 | + | 738 | WP_006472387 | P-type conjugative transfer protein TrbJ | virB5 |
| AN232_RS28300 (AN232_28300) | 60460..60786 | + | 327 | WP_023106769 | hypothetical protein | - |
| AN232_RS28305 (AN232_28305) | 60783..62159 | + | 1377 | WP_023106768 | P-type conjugative transfer protein TrbL | virB6 |
| AN232_RS28310 (AN232_28310) | 62172..62876 | + | 705 | WP_006472385 | conjugal transfer protein TrbF | virB8 |
| AN232_RS28315 (AN232_28315) | 62873..63865 | + | 993 | WP_006472384 | P-type conjugative transfer protein TrbG | virB9 |
| AN232_RS28320 (AN232_28320) | 63868..65148 | + | 1281 | WP_006472383 | TrbI/VirB10 family protein | virB10 |
| AN232_RS28325 (AN232_28325) | 65145..65387 | + | 243 | WP_006472382 | DUF2274 domain-containing protein | - |
| AN232_RS31880 (AN232_28330) | 65609..65857 | - | 249 | WP_204354936 | hypothetical protein | - |
| AN232_RS28335 (AN232_28335) | 65883..66740 | - | 858 | WP_001167036 | hypothetical protein | - |
| AN232_RS28340 (AN232_28340) | 66727..66957 | - | 231 | WP_000972665 | hypothetical protein | - |
| AN232_RS28345 (AN232_28345) | 66957..67475 | - | 519 | WP_000210757 | nitrite reductase | - |
| AN232_RS28350 (AN232_28350) | 67472..67918 | - | 447 | WP_000919343 | hypothetical protein | - |
| AN232_RS28355 (AN232_28355) | 67918..68277 | - | 360 | WP_000422769 | hypothetical protein | - |
| AN232_RS28360 (AN232_28360) | 68335..68763 | - | 429 | WP_000591076 | hypothetical protein | - |
| AN232_RS28365 (AN232_28365) | 68797..69657 | - | 861 | WP_000139696 | DsbA family protein | - |
Host bacterium
| ID | 14869 | GenBank | NZ_CP029730 |
| Plasmid name | AR_0157|unnamed2 | Incompatibility group | IncA/C2 |
| Plasmid size | 136246 bp | Coordinate of oriT [Strand] | 78837..78941 [-] |
| Host baterium | Citrobacter sp. CRE-46 strain AR_0157 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |