Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114433
Name   oriT_AR_0157|unnamed1 in_silico
Organism   Citrobacter sp. CRE-46 strain AR_0157
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP029729 (53885..54169 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_AR_0157|unnamed1
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9253 GenBank   WP_001011148
Name   mobH_AN232_RS26800_AR_0157|unnamed1 insolico UniProt ID   A0A377MSW1
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 113004.00 Da        Isoelectric Point: 4.5009

>WP_001011148.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKSKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENDIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTEAITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAEFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPRPRLNAPVAEEASSVAEQTNAHSEPESVIATEYEQYGHLLEETLESDGEAYSDLIASDSTEAEYPATD
PQSSDFAQLPGETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPIPIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure


Source ID Structure
AlphaFold DB A0A377MSW1


T4CP

ID   10722 GenBank   WP_000167421
Name   traD_AN232_RS26805_AR_0157|unnamed1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77946.71 Da        Isoelectric Point: 8.7275

>WP_000167421.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKTNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKSAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 116539..125707

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS27160 (AN232_27160) 112556..113431 + 876 WP_001282731 RepB family plasmid replication initiator protein -
AN232_RS31550 115139..115483 - 345 WP_153253765 hypothetical protein -
AN232_RS27175 (AN232_27175) 116134..116487 + 354 WP_000424120 pili assembly chaperone -
AN232_RS27180 (AN232_27180) 116539..116856 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
AN232_RS27185 (AN232_27185) 116868..117653 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
AN232_RS27190 (AN232_27190) 117656..118888 + 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
AN232_RS27195 (AN232_27195) 118890..119330 + 441 WP_001224095 plasmid transfer protein HtdO -
AN232_RS27200 (AN232_27200) 119320..120678 + 1359 WP_000351839 IncHI-type conjugal transfer protein TrhB traB
AN232_RS27205 (AN232_27205) 120687..121199 + 513 WP_000429933 hypothetical protein -
AN232_RS27210 (AN232_27210) 121305..122057 + 753 WP_010892304 protein-disulfide isomerase HtdT -
AN232_RS27215 (AN232_27215) 122067..123017 + 951 WP_001022266 TraV family lipoprotein traV
AN232_RS27220 (AN232_27220) 123026..125707 + 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
AN232_RS27225 (AN232_27225) 125911..126114 + 204 WP_001395470 hypothetical protein -
AN232_RS27230 (AN232_27230) 126552..127349 - 798 WP_000544821 IS21-like element IS21 family helper ATPase IstB -
AN232_RS27235 (AN232_27235) 127349..128521 - 1173 WP_000952439 IS21-like element IS21 family transposase -
AN232_RS31555 128671..128937 + 267 WP_137474779 hypothetical protein -
AN232_RS31560 129615..129788 + 174 WP_153801876 hypothetical protein -
AN232_RS31565 129797..130168 - 372 WP_162620748 hypothetical protein -
AN232_RS31570 130266..130412 + 147 WP_162620746 hypothetical protein -

Host bacterium

ID   14868 GenBank   NZ_CP029729
Plasmid name   AR_0157|unnamed1 Incompatibility group   IncHI1B
Plasmid size   250887 bp Coordinate of oriT [Strand]   53885..54169 [-]
Host baterium   Citrobacter sp. CRE-46 strain AR_0157

Cargo genes

Drug resistance gene   blaCTX-M-15, mph(E), msr(E), armA, sul1, qacE, aadA2, dfrA12, aac(6')-Ib, ant(3'')-Ia, blaOXA-9, blaTEM-1A
Virulence gene   -
Metal resistance gene   arsR, arsD, terW, terZ, terA, terB, terC, terD, terE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -