Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114422
• Name: oriT_pS11L-3
• Organism: Bradyrhizobium japonicum strain S11L-Bj
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP126016 (109142..109183 [-], 42 nt)
• oriT length: 42 nt
• IRs (inverted repeats): 9..15, 20..26 (ACGTCGC..GCGACGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 42 nt

>oriT_pS11L-3
AAAGCAGGACGTCGCGGATGCGACGTATTACTGCGCCCTTGG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_QIH95_RS51765_pS11L-3

• ID: 9242
• GenBank: WP_212479306
• Name: traA_QIH95_RS51765_pS11L-3
• UniProt ID: _
• Length: 1102 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1102 a.a.
• Molecular weight: 121918.26 Da
• Isoelectric Point: 9.9984

>WP_212479306.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Bradyrhizobium]
MAITHFTPQLISRGSGRSAVLSAAYRHCARMEHQAEGRTVDYSAKRGLRYEEFLLPPDAPQWARELIADR
SVAGAAEAFWNAIEAFEKRSDAQLAKEFIIALPVELTIDQNVALVREFVATQVLARGQVADWVFHDEPGN
PHIHLMTTLRPLGEDGFGPKKVPVIGHDGAPLRNAAGKIVYRLWSGEKAEFLAQREGWLDLQNRHLALAG
LDIRVDGRSYAERGVDLAPTTHIGVATKAIDRKGKKAGWSPKLERVALHEERKTDNRQRILRRPEIVLDL
VSREMSVFTERDIARVLHRYVDDAGTFQHLMARILQNPEALRLEGECIALATGIREPAKYTTRELIRLEA
EMAHRAVWLAGRSSHGVREKVLAAAFERHARLSEEQRVAIAHVASVGRIAAVVGRAGAGKTTMMKAAREV
WEAAGYRVVGAALAGKAAEGLEKEAGIASRTLASWELAWSNGREQLDDKTIFVLDEAGMVSSRQMALFVE
AVVKTGAKLALVGDPDQLQPIEAGAAFRAIAERTGYAELETIYRQREQWMRDASLDLARGRVAEALQAYG
ANGKLEARKYKAQAVEALIEDWNWDYDPAKSTLILAHLRRDVRVLNEMARAKLIERGIVEDGHAFRTEDG
PRSFATGDQIVFLKNEGSLGVKNGMIARVIEARPGWFAAMIGEGEARRRVEVDQRFYANVDHGYATTIHK
SQGATVDRVKVLATLSLDRHLAYVALTRHREDVALYYGAISFGKAGGLTKILSQSRAKETTLDYERGPSY
RAALRFAEARGLHLMRVARTLVADKVRWTVRQKQKLADLGARLLALGARLGLGAARKQTIPNGAKEAKPM
VAGITTFAKSIDQVVEDKLAADPGLKKQWEEVSTRFRFVFAEPESAFRKVDVDSMLKDKATAQSTLSKLA
EKPESFGALKGKTGLLASRADKQDRQRADLNVPALARDLERFLRLRSEAEHRYEAEERAARLKVAVDIPA
LSPSAKQTLERIRDAIDRNDLPAALEFALEDKMIEAELEGFARAVSERFGERSFVGLAAKDTDGKTFETL
SGGMNAAQKEGLKAAWPDLRTAQQLAAQQRTAVALKEAKTLRQTQSKGLSLQ

Protein domains

Predicted by InterproScan.

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (692-735)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (858-954)

• AAA domain (AAA_30) (382-566)

• MobA/MobL family (MobA_MobL) (17-243)

Protein structure

No available structure.

T4CP

t4cp1_QIH95_RS51685_pS11L-3

• ID: 10706
• GenBank: WP_244430258
• Name: t4cp1_QIH95_RS51685_pS11L-3
• UniProt ID: _
• Length: 353 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 353 a.a.
• Molecular weight: 38609.45 Da
• Isoelectric Point: 9.9739

>WP_244430258.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Bradyrhizobium]
MTPFANATTPVKAALGTALALGAVAVFLLVASNVLLLGLRLHDGGIHWSEIAKGWPRYGIDPRFDRWLTL
STLAGSIVVFGLLGAILRHRPLPLHGKARFASEREIKAAGLRSKDGLLLGRKDGAMLCFGGSEHVLLYAP
TRAGKGVGYVIPNLLNWPDSVVALDVKKENWDRSAGFRAAHGQEIHLFDPLDENGRTARYNPLGYIRTDP
ADLYDDLQRIAVMLFPAESRGDPFWFEAARSAFVAIGGYVAETPGLPLTIGETLRQLSATQDLKAHFDRI
IADRKAGPSPLSRHCITALNDFLAASENTLNSVRKTVTARLGLWLNPRIDAATSTNDFDLRQLRRRPMSI
YLG

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (101-352)

Protein structure

No available structure.

traG_QIH95_RS51750_pS11L-3

• ID: 10707
• GenBank: WP_248890198
• Name: traG_QIH95_RS51750_pS11L-3
• UniProt ID: _
• Length: 640 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 640 a.a.
• Molecular weight: 68705.10 Da
• Isoelectric Point: 9.9887

>WP_248890198.1 Ti-type conjugative transfer system protein TraG [Bradyrhizobium japonicum]
MTANRLLLLIVPMALMLGAIFGLTGMGHGLAGLGKTEAQRLLVARAGIAIPYIVAAAIGVVFLFAAAGSA
NIRTAAWSVVAGCAAAISMAAAREAIRLFAVPATAIPGRPPLVQIDPAIMVGAGIPLMAGIFALRVAVKG
NAAFTTPMPKRVRGRRALHGDADWMSMPEAARLFGIAGGIVVGERYRVDKDNAAAQPFRAMDRQSWGHGG
RAPLLCFDATFGSSHGIVFAGSGGFKTTSVTIPTALKWGGALVVLDPSNEVAPMVIKHRRAAGRTVHVLD
PKESGTGFNALDWIGQFGGRKEEDIAAVASWIMSDSGGARGVRDDFFRASALQLLTALIADVCLSGHTEE
KNQTLRQVRANLSEPEPKLRARLQAIYDNSGSDFVKENVAVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGSTFVSDDLAGGTTDVFINLDLKTLETHAGLARVIIGSFMNAIYNRNGEVTGRALFLLDEVARLGF
MRILETARDAGRKYGITLLLLFQSIGQMRETYGGRDAASKWFESASWIAFAAVNDPETADYISKRCGMTT
VEIDQVSRSSQASGSSRTRSKQLASRPLIQPHEVLRMRADEQIVFTAGNAPLRCGRAIWFRRDDMRACVG
KNRFQPEEKT

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (164-635)

Protein structure

No available structure.

t4cp1_QIH95_RS52130_pS11L-3

• ID: 10708
• GenBank: WP_244430258
• Name: t4cp1_QIH95_RS52130_pS11L-3
• UniProt ID: _
• Length: 353 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 353 a.a.
• Molecular weight: 38609.45 Da
• Isoelectric Point: 9.9739

>WP_244430258.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Bradyrhizobium]
MTPFANATTPVKAALGTALALGAVAVFLLVASNVLLLGLRLHDGGIHWSEIAKGWPRYGIDPRFDRWLTL
STLAGSIVVFGLLGAILRHRPLPLHGKARFASEREIKAAGLRSKDGLLLGRKDGAMLCFGGSEHVLLYAP
TRAGKGVGYVIPNLLNWPDSVVALDVKKENWDRSAGFRAAHGQEIHLFDPLDENGRTARYNPLGYIRTDP
ADLYDDLQRIAVMLFPAESRGDPFWFEAARSAFVAIGGYVAETPGLPLTIGETLRQLSATQDLKAHFDRI
IADRKAGPSPLSRHCITALNDFLAASENTLNSVRKTVTARLGLWLNPRIDAATSTNDFDLRQLRRRPMSI
YLG

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (101-352)

Protein structure

No available structure.

t4cp2_QIH95_RS52485_pS11L-3

• ID: 10709
• GenBank: WP_248890149
• Name: t4cp2_QIH95_RS52485_pS11L-3
• UniProt ID: _
• Length: 392 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 392 a.a.
• Molecular weight: 43523.08 Da
• Isoelectric Point: 9.4312

>WP_248890149.1 type IV secretory system conjugative DNA transfer family protein [Bradyrhizobium japonicum]
MELESEIARSGNPDSSQKHHALKAHFDKIIANRKGGLSPLSRHCITALNDLLAASENTLNSVRKTVTARL
GLWLNPRIDAATLSNDFDLRQLRQQPMSIYLGVTPDNLDRMAPLLNLFFQQVVDLNTRELPEQNPKLTRK
VLLLLDEFPALGNVNVLAKSVAFRYGIRLLTVVQSPAQLRAIYGIDAARNFMTNHAVEVVFAPKEQDVAN
ELSERIGYETVNARSRSGPKGLAMRATSETVSEHRRALMLPQELKLLPKSKAFILGTGIPPIIADKIVYY
EDKAFLQRLLPAPVPELPKGRSNALLDAELKKLRSEIAELRAVFRARPMTDDEVADPSTIPANASFDFGD
VDIDLEGLSEDGMKAWTLNYIDAQAIPPARRSGPKREKRVAV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (46-289)

Protein structure

No available structure.

Host bacterium

• ID: 14857
• GenBank: NZ_CP126016
• Plasmid name: pS11L-3
• Incompatibility group: -
• Plasmid size: 266016 bp
• Coordinate of oriT [Strand]: 109142..109183 [-]
• Host baterium: Bradyrhizobium japonicum strain S11L-Bj

Cargo genes

• Drug resistance gene: -
• Virulence gene: bscN
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -