Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114409
• Name: oriT_pF179_3
• Organism: Enterococcus faecium strain F179
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP072887 (8275..8317 [-], 43 nt)
• oriT length: 43 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 43 nt

>oriT_pF179_3
AATATCGCAACATGGGCCCATGTTGCTCTGCTCGCAAAAGAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_J9540_RS13675_pF179_3

• ID: 9232
• GenBank: WP_002340563
• Name: Relaxase_J9540_RS13675_pF179_3
• UniProt ID: A1IGF3
• Length: 304 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 304 a.a.
• Molecular weight: 35643.44 Da
• Isoelectric Point: 9.9699

>WP_002340563.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRMLYGKDDRVQAHLLIQSFRPGEITAE
KANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHN
LPITERGKKPEKRTMSEIKLKEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFT
YELIHKKKKVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPE
IRPARRNHEQHVERGTDHQQELSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-241)

Protein structure

• AlphaFold DB: A1IGF3

Host bacterium

• ID: 14844
• GenBank: NZ_CP072887
• Plasmid name: pF179_3
• Incompatibility group: -
• Plasmid size: 26651 bp
• Coordinate of oriT [Strand]: 8275..8317 [-]
• Host baterium: Enterococcus faecium strain F179

Cargo genes

• Drug resistance gene: fexB, poxtA
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9