Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114407
Name   oriT_pSK37064 in_silico
Organism   Virgibacillus sp. SK37
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP007163 (3177..3214 [-], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      14..21, 28..35  (AAAGTATA..TATACTTT)
 2..8, 12..18  (ACTTTTG..CAAAAGT)
Location of nic site      26..27
Conserved sequence flanking the
  nic site    		  GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pSK37064
CACTTTTGGAACAAAAGTATAGTGTGTTATACTTTACA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9230 GenBank   WP_040957274
Name   mobV_X953_RS18930_pSK37064 insolico UniProt ID   A0A075K0G7
Length   402 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 402 a.a.        Molecular weight: 46988.33 Da        Isoelectric Point: 9.6825

>WP_040957274.1 MobV family relaxase [Virgibacillus sp. SK37]
MSYAVCRMAKMKSHSLKGMQIHNQREKESKTNPDIDKSKLSLNYDLANDEPINYNEKVKQIIDSQKTSTR
KTRKDAVLVNELLVTSDRKFFDNLSSEEQKRFFEESYKLFSERYGQQNVAYATVHNDEKTPHMHLGIVPM
RDGKLQGKNVFNQKELRWIQDEFPKHLQSKGFDIERGEKGSTAEHLKVQDFKAKTLEERIAGLETELGEK
QKEKVHVEQSIEHIQNELGALQKASIHSKGVEGIEFKEKGLLGRKTVNLERSDFEELKSLAKVSESLQIK
NDKLENKNVQLEKLNWNLRKKNDHLTRENTKLNKENKSLVQENKFLSRTLENVKVRFRDQVKDFHSKIGF
IKANILDKMEMEIFKKYFGGQEELEGGQAFLALKKQQEKEKQNQKEKDDFEL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB A0A075K0G7


Host bacterium

ID   14842 GenBank   NZ_CP007163
Plasmid name   pSK37064 Incompatibility group   -
Plasmid size   4094 bp Coordinate of oriT [Strand]   3177..3214 [-]
Host baterium   Virgibacillus sp. SK37

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -