Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114375
Name   oriT_pSa27-HP in_silico
Organism   Salmonella sp. strain Sa27
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MH884654 (55688..55776 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSa27-HP
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9216 GenBank   WP_001306778
Name   nikB_HXJ06_RS00275_pSa27-HP insolico UniProt ID   Q79VV7
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_001306778.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB Q79VV7


Auxiliary protein

ID   5402 GenBank   WP_001283947
Name   WP_001283947_pSa27-HP insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   10682 GenBank   WP_001289276
Name   trbC_HXJ06_RS00270_pSa27-HP insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12013..50310

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXJ06_RS00045 (EPNHJBFF_00504) 7075..8142 + 1068 WP_021513968 type IV pilus biogenesis lipoprotein PilL -
HXJ06_RS00050 (EPNHJBFF_00505) 8142..8579 + 438 WP_000539811 type IV pilus biogenesis protein PilM -
HXJ06_RS00055 (EPNHJBFF_00506) 8593..10275 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HXJ06_RS00060 (EPNHJBFF_00507) 10268..11563 + 1296 WP_000752776 type 4b pilus protein PilO2 -
HXJ06_RS00065 (EPNHJBFF_00508) 11550..12002 + 453 WP_001247333 type IV pilus biogenesis protein PilP -
HXJ06_RS00070 (EPNHJBFF_00509) 12013..13566 + 1554 WP_000362204 GspE/PulE family protein virB11
HXJ06_RS00075 (EPNHJBFF_00510) 13579..14664 + 1086 WP_001208803 type II secretion system F family protein -
HXJ06_RS00080 (EPNHJBFF_00511) 14681..15295 + 615 WP_000908228 type 4 pilus major pilin -
HXJ06_RS00085 (EPNHJBFF_00512) 15305..15865 + 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
HXJ06_RS00090 (EPNHJBFF_00513) 15850..16506 + 657 WP_001193551 A24 family peptidase -
HXJ06_RS00095 (EPNHJBFF_00514) 16494..17930 + 1437 WP_015058913 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HXJ06_RS00100 17927..18149 - 223 Protein_20 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HXJ06_RS00105 18202..18459 + 258 WP_001302706 hypothetical protein -
HXJ06_RS00110 18749..18904 - 156 WP_001302705 hypothetical protein -
HXJ06_RS00505 19255..19524 - 270 Protein_23 hypothetical protein -
HXJ06_RS00115 (EPNHJBFF_00516) 19564..20718 + 1155 WP_001139955 tyrosine-type recombinase/integrase -
HXJ06_RS00120 (EPNHJBFF_00517) 20869..21693 + 825 WP_015058912 conjugal transfer protein TraE traE
HXJ06_RS00125 (EPNHJBFF_00518) 21779..22981 + 1203 WP_000976357 conjugal transfer protein TraF -
HXJ06_RS00130 (EPNHJBFF_00519) 23041..23625 + 585 WP_000977520 histidine phosphatase family protein -
HXJ06_RS00135 (EPNHJBFF_00520) 24020..24478 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HXJ06_RS00140 (EPNHJBFF_00521) 24475..25293 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
HXJ06_RS00145 (EPNHJBFF_00522) 25290..26438 + 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
HXJ06_RS00150 (EPNHJBFF_00523) 26435..26725 + 291 WP_001299214 hypothetical protein traK
HXJ06_RS00155 (EPNHJBFF_00524) 26740..27291 + 552 WP_000014584 phospholipase D family protein -
HXJ06_RS00160 (EPNHJBFF_00525) 27381..31148 + 3768 WP_006855639 LPD7 domain-containing protein -
HXJ06_RS00165 (EPNHJBFF_00526) 31166..31513 + 348 WP_001055900 conjugal transfer protein traL
HXJ06_RS00170 (EPNHJBFF_00527) 31510..32202 + 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
HXJ06_RS00175 (EPNHJBFF_00528) 32213..33196 + 984 WP_176704381 IncI1-type conjugal transfer protein TraN traN
HXJ06_RS00180 (EPNHJBFF_00529) 33199..34488 + 1290 WP_001271994 conjugal transfer protein TraO traO
HXJ06_RS00185 (EPNHJBFF_00530) 34488..35192 + 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
HXJ06_RS00190 (EPNHJBFF_00531) 35192..35719 + 528 WP_001055569 conjugal transfer protein TraQ traQ
HXJ06_RS00195 (EPNHJBFF_00532) 35770..36174 + 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
HXJ06_RS00200 (EPNHJBFF_00533) 36238..36426 + 189 WP_001277255 putative conjugal transfer protein TraS -
HXJ06_RS00205 (EPNHJBFF_00534) 36410..37210 + 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
HXJ06_RS00210 (EPNHJBFF_00535) 37300..40344 + 3045 WP_001024776 IncI1-type conjugal transfer protein TraU traU
HXJ06_RS00215 (EPNHJBFF_00536) 40344..40958 + 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
HXJ06_RS00220 (EPNHJBFF_00537) 40925..42127 + 1203 WP_001385654 IncI1-type conjugal transfer protein TraW traW
HXJ06_RS00225 (EPNHJBFF_00538) 42156..42740 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HXJ06_RS00230 (EPNHJBFF_00539) 42837..45005 + 2169 WP_000698354 DotA/TraY family protein traY
HXJ06_RS00235 (EPNHJBFF_00540) 45076..45738 + 663 WP_000644796 plasmid IncI1-type surface exclusion protein ExcA -
HXJ06_RS00240 (EPNHJBFF_00541) 45810..46019 - 210 WP_039025903 HEAT repeat domain-containing protein -
HXJ06_RS00245 46303..46587 + 285 WP_001707309 hypothetical protein -
HXJ06_RS00490 (EPNHJBFF_00543) 46652..46747 - 96 WP_001303310 DinQ-like type I toxin DqlB -
HXJ06_RS00250 (EPNHJBFF_00544) 47246..47497 + 252 WP_001291964 hypothetical protein -
HXJ06_RS00255 (EPNHJBFF_00545) 47569..47721 - 153 WP_001331364 Hok/Gef family protein -
HXJ06_RS00260 (EPNHJBFF_00546) 48013..49221 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HXJ06_RS00265 (EPNHJBFF_00547) 49240..50310 + 1071 WP_000151582 IncI1-type conjugal transfer protein TrbB trbB
HXJ06_RS00270 (EPNHJBFF_00548) 50303..52594 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Host bacterium

ID   14810 GenBank   NZ_MH884654
Plasmid name   pSa27-HP Incompatibility group   IncI1
Plasmid size   83480 bp Coordinate of oriT [Strand]   55688..55776 [+]
Host baterium   Salmonella sp. strain Sa27

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -