Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   114372
Name   oriT_pKSR100 in_silico
Organism   Shigella flexneri strain SF7955
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LN624486 (19473..19824 [+], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 104..109, 119..124  (TTTAAA..TTTAAA)
 93..98, 106..111  (GATTTA..TAAATC)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_pKSR100
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTTTTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   9213 GenBank   WP_176453900
Name   traI_HXH51_RS00330_pKSR100 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191530.02 Da        Isoelectric Point: 5.6895

>WP_176453900.1 conjugative transfer relaxase/helicase TraI [Shigella flexneri]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMQNGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGIGKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMSAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGEGLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVRPGAGNGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAGNKPDLP
DGKTEQAVREIAGQERDRTATSERETALPESVLRESQREQEAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(1434-1557)

(633-712)

(10-283)

(968-1156)

(575-626)


  Protein structure



No available structure.




Auxiliary protein


ID   5399 GenBank   WP_001151529
Name   WP_001151529_pKSR100 insolico UniProt ID   A0A7I0L277
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14497.49 Da        Isoelectric Point: 4.8662

>WP_001151529.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSAEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L277


T4CP


ID   10678 GenBank   WP_000069772
Name   traC_HXH51_RS00220_pKSR100 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99344.15 Da        Isoelectric Point: 6.4096

>WP_000069772.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(291-447)

(468-764)

(39-277)

  Protein structure



No available structure.



ID   10679 GenBank   WP_125093769
Name   traD_HXH51_RS00325_pKSR100 insolico UniProt ID   _
Length   744 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 744 a.a.        Molecular weight: 84725.55 Da        Isoelectric Point: 5.2345

>WP_125093769.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIA
EFAAGEIGEKEHLKASLQYSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
DMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(32-128)

(173-560)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 18902..48314

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXH51_RS00105 14874..15308 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
HXH51_RS00110 15305..16067 + 763 Protein_23 plasmid SOS inhibition protein A -
HXH51_RS00480 16246..16395 + 150 Protein_24 plasmid maintenance protein Mok -
HXH51_RS00115 16337..16462 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
HXH51_RS00120 16781..17077 - 297 Protein_26 hypothetical protein -
HXH51_RS00125 17377..17673 + 297 WP_001272251 hypothetical protein -
HXH51_RS00130 17784..18605 + 822 WP_001234445 DUF932 domain-containing protein -
HXH51_RS00135 18902..19504 - 603 WP_000243713 transglycosylase SLT domain-containing protein virB1
HXH51_RS00140 19825..20208 + 384 WP_001151529 conjugal transfer relaxosome DNA-binding protein TraM -
HXH51_RS00145 20400..21047 + 648 WP_000332523 transcriptional regulator TraJ family protein -
HXH51_RS00150 21167..21394 + 228 WP_000589556 conjugal transfer relaxosome protein TraY -
HXH51_RS00155 21428..21787 + 360 WP_001098994 type IV conjugative transfer system pilin TraA -
HXH51_RS00160 21802..22113 + 312 WP_000012113 type IV conjugative transfer system protein TraL traL
HXH51_RS00165 22135..22701 + 567 WP_000399780 type IV conjugative transfer system protein TraE traE
HXH51_RS00170 22688..23416 + 729 WP_001230769 type-F conjugative transfer system secretin TraK traK
HXH51_RS00175 23416..24843 + 1428 WP_033807957 F-type conjugal transfer pilus assembly protein TraB traB
HXH51_RS00180 24833..25423 + 591 WP_000002784 conjugal transfer pilus-stabilizing protein TraP -
HXH51_RS00185 25410..25730 + 321 WP_012881128 conjugal transfer protein TrbD -
HXH51_RS00190 25727..26242 + 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
HXH51_RS00195 26377..26598 + 222 WP_001278683 conjugal transfer protein TraR -
HXH51_RS00200 26591..27004 + 414 WP_000549589 hypothetical protein -
HXH51_RS00205 26997..27470 + 474 WP_033807958 hypothetical protein -
HXH51_RS00210 27569..27787 + 219 WP_033807959 hypothetical protein -
HXH51_RS00215 27815..28162 + 348 WP_000836682 hypothetical protein -
HXH51_RS00220 28288..30918 + 2631 WP_000069772 type IV secretion system protein TraC virb4
HXH51_RS00225 30915..31301 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
HXH51_RS00230 31298..31930 + 633 WP_001203720 type-F conjugative transfer system protein TraW traW
HXH51_RS00235 31927..32919 + 993 WP_000830849 conjugal transfer pilus assembly protein TraU traU
HXH51_RS00240 32949..33254 + 306 WP_000224418 hypothetical protein -
HXH51_RS00245 33263..33901 + 639 WP_033807960 type-F conjugative transfer system pilin assembly protein TrbC trbC
HXH51_RS00250 33898..34278 + 381 WP_001462111 hypothetical protein -
HXH51_RS00255 34514..36418 + 1905 WP_225620370 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HXH51_RS00260 36445..36702 + 258 WP_033807961 conjugal transfer protein TrbE -
HXH51_RS00265 36695..37438 + 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
HXH51_RS00270 37452..37793 + 342 WP_000556795 conjugal transfer protein TrbA -
HXH51_RS00275 37774..38109 - 336 WP_001620807 hypothetical protein -
HXH51_RS00280 38190..38474 + 285 WP_000624107 type-F conjugative transfer system pilin chaperone TraQ -
HXH51_RS00285 38461..39006 + 546 WP_000059829 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HXH51_RS00290 38936..39283 + 348 WP_071571857 P-type conjugative transfer protein TrbJ -
HXH51_RS00295 39264..39656 + 393 WP_000659962 F-type conjugal transfer protein TrbF -
HXH51_RS00300 39643..41016 + 1374 WP_033807962 conjugal transfer pilus assembly protein TraH traH
HXH51_RS00305 41013..43838 + 2826 WP_033807963 conjugal transfer mating-pair stabilization protein TraG traG
HXH51_RS00310 43835..44344 + 510 WP_000628111 conjugal transfer entry exclusion protein TraS -
HXH51_RS00315 44358..45089 + 732 WP_012881123 conjugal transfer complement resistance protein TraT -
HXH51_RS00320 45292..46029 + 738 WP_012881122 hypothetical protein -
HXH51_RS00325 46080..48314 + 2235 WP_125093769 type IV conjugative transfer system coupling protein TraD prgC


Host bacterium


ID   14807 GenBank   NZ_LN624486
Plasmid name   pKSR100 Incompatibility group   -
Plasmid size   73047 bp Coordinate of oriT [Strand]   19473..19824 [+]
Host baterium   Shigella flexneri strain SF7955

Cargo genes


Drug resistance gene   blaTEM-1B, mph(A), erm(B)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -