Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 114372 |
Name | oriT_pKSR100 |
Organism | Shigella flexneri strain SF7955 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_LN624486 (19473..19824 [+], 352 nt) |
oriT length | 352 nt |
IRs (inverted repeats) | 192..198, 206..212 (TATAAAA..TTTTATA) 104..109, 119..124 (TTTAAA..TTTAAA) 93..98, 106..111 (GATTTA..TAAATC) 40..47, 50..57 (GCAAAAAC..GTTTTTGC) 4..11, 16..23 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 352 nt
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTTTTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 9213 | GenBank | WP_176453900 |
Name | traI_HXH51_RS00330_pKSR100 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191530.02 Da Isoelectric Point: 5.6895
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMQNGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGIGKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMSAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGEGLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVRPGAGNGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAGNKPDLP
DGKTEQAVREIAGQERDRTATSERETALPESVLRESQREQEAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 5399 | GenBank | WP_001151529 |
Name | WP_001151529_pKSR100 | UniProt ID | A0A7I0L277 |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14497.49 Da Isoelectric Point: 4.8662
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSAEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7I0L277 |
T4CP
ID | 10678 | GenBank | WP_000069772 |
Name | traC_HXH51_RS00220_pKSR100 | UniProt ID | _ |
Length | 876 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 876 a.a. Molecular weight: 99344.15 Da Isoelectric Point: 6.4096
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 10679 | GenBank | WP_125093769 |
Name | traD_HXH51_RS00325_pKSR100 | UniProt ID | _ |
Length | 744 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 744 a.a. Molecular weight: 84725.55 Da Isoelectric Point: 5.2345
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIA
EFAAGEIGEKEHLKASLQYSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
DMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 18902..48314
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HXH51_RS00105 | 14874..15308 | + | 435 | WP_000845953 | conjugation system SOS inhibitor PsiB | - |
HXH51_RS00110 | 15305..16067 | + | 763 | Protein_23 | plasmid SOS inhibition protein A | - |
HXH51_RS00480 | 16246..16395 | + | 150 | Protein_24 | plasmid maintenance protein Mok | - |
HXH51_RS00115 | 16337..16462 | + | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
HXH51_RS00120 | 16781..17077 | - | 297 | Protein_26 | hypothetical protein | - |
HXH51_RS00125 | 17377..17673 | + | 297 | WP_001272251 | hypothetical protein | - |
HXH51_RS00130 | 17784..18605 | + | 822 | WP_001234445 | DUF932 domain-containing protein | - |
HXH51_RS00135 | 18902..19504 | - | 603 | WP_000243713 | transglycosylase SLT domain-containing protein | virB1 |
HXH51_RS00140 | 19825..20208 | + | 384 | WP_001151529 | conjugal transfer relaxosome DNA-binding protein TraM | - |
HXH51_RS00145 | 20400..21047 | + | 648 | WP_000332523 | transcriptional regulator TraJ family protein | - |
HXH51_RS00150 | 21167..21394 | + | 228 | WP_000589556 | conjugal transfer relaxosome protein TraY | - |
HXH51_RS00155 | 21428..21787 | + | 360 | WP_001098994 | type IV conjugative transfer system pilin TraA | - |
HXH51_RS00160 | 21802..22113 | + | 312 | WP_000012113 | type IV conjugative transfer system protein TraL | traL |
HXH51_RS00165 | 22135..22701 | + | 567 | WP_000399780 | type IV conjugative transfer system protein TraE | traE |
HXH51_RS00170 | 22688..23416 | + | 729 | WP_001230769 | type-F conjugative transfer system secretin TraK | traK |
HXH51_RS00175 | 23416..24843 | + | 1428 | WP_033807957 | F-type conjugal transfer pilus assembly protein TraB | traB |
HXH51_RS00180 | 24833..25423 | + | 591 | WP_000002784 | conjugal transfer pilus-stabilizing protein TraP | - |
HXH51_RS00185 | 25410..25730 | + | 321 | WP_012881128 | conjugal transfer protein TrbD | - |
HXH51_RS00190 | 25727..26242 | + | 516 | WP_000809881 | type IV conjugative transfer system lipoprotein TraV | traV |
HXH51_RS00195 | 26377..26598 | + | 222 | WP_001278683 | conjugal transfer protein TraR | - |
HXH51_RS00200 | 26591..27004 | + | 414 | WP_000549589 | hypothetical protein | - |
HXH51_RS00205 | 26997..27470 | + | 474 | WP_033807958 | hypothetical protein | - |
HXH51_RS00210 | 27569..27787 | + | 219 | WP_033807959 | hypothetical protein | - |
HXH51_RS00215 | 27815..28162 | + | 348 | WP_000836682 | hypothetical protein | - |
HXH51_RS00220 | 28288..30918 | + | 2631 | WP_000069772 | type IV secretion system protein TraC | virb4 |
HXH51_RS00225 | 30915..31301 | + | 387 | WP_000214084 | type-F conjugative transfer system protein TrbI | - |
HXH51_RS00230 | 31298..31930 | + | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
HXH51_RS00235 | 31927..32919 | + | 993 | WP_000830849 | conjugal transfer pilus assembly protein TraU | traU |
HXH51_RS00240 | 32949..33254 | + | 306 | WP_000224418 | hypothetical protein | - |
HXH51_RS00245 | 33263..33901 | + | 639 | WP_033807960 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
HXH51_RS00250 | 33898..34278 | + | 381 | WP_001462111 | hypothetical protein | - |
HXH51_RS00255 | 34514..36418 | + | 1905 | WP_225620370 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
HXH51_RS00260 | 36445..36702 | + | 258 | WP_033807961 | conjugal transfer protein TrbE | - |
HXH51_RS00265 | 36695..37438 | + | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
HXH51_RS00270 | 37452..37793 | + | 342 | WP_000556795 | conjugal transfer protein TrbA | - |
HXH51_RS00275 | 37774..38109 | - | 336 | WP_001620807 | hypothetical protein | - |
HXH51_RS00280 | 38190..38474 | + | 285 | WP_000624107 | type-F conjugative transfer system pilin chaperone TraQ | - |
HXH51_RS00285 | 38461..39006 | + | 546 | WP_000059829 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
HXH51_RS00290 | 38936..39283 | + | 348 | WP_071571857 | P-type conjugative transfer protein TrbJ | - |
HXH51_RS00295 | 39264..39656 | + | 393 | WP_000659962 | F-type conjugal transfer protein TrbF | - |
HXH51_RS00300 | 39643..41016 | + | 1374 | WP_033807962 | conjugal transfer pilus assembly protein TraH | traH |
HXH51_RS00305 | 41013..43838 | + | 2826 | WP_033807963 | conjugal transfer mating-pair stabilization protein TraG | traG |
HXH51_RS00310 | 43835..44344 | + | 510 | WP_000628111 | conjugal transfer entry exclusion protein TraS | - |
HXH51_RS00315 | 44358..45089 | + | 732 | WP_012881123 | conjugal transfer complement resistance protein TraT | - |
HXH51_RS00320 | 45292..46029 | + | 738 | WP_012881122 | hypothetical protein | - |
HXH51_RS00325 | 46080..48314 | + | 2235 | WP_125093769 | type IV conjugative transfer system coupling protein TraD | prgC |
Host bacterium
ID | 14807 | GenBank | NZ_LN624486 |
Plasmid name | pKSR100 | Incompatibility group | - |
Plasmid size | 73047 bp | Coordinate of oriT [Strand] | 19473..19824 [+] |
Host baterium | Shigella flexneri strain SF7955 |
Cargo genes
Drug resistance gene | blaTEM-1B, mph(A), erm(B) |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |