Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114353
• Name: oriT_pRlBT01c
• Organism: Rhizobium leguminosarum strain BT01
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP125638 (332595..332623 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pRlBT01c
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_QMO81_RS34110_pRlBT01c

• ID: 9201
• GenBank: WP_283187232
• Name: traA_QMO81_RS34110_pRlBT01c
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 169784.76 Da
• Isoelectric Point: 7.2770

>WP_283187232.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVIGKGAGRSVVSAAAYRHRTRMMDEQAGTSFSYRGGASELVHEELALPDQIPAWLRSAISG
QSVAKASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVLDNLTAKGMVADWVYHDKDG
NPHIHLMTTLRPLAEEGFGPKKVPVRGEDGKPLRVVTPDRPNGKVVYKLWAGDKETMKGWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRDAEMYFAPADLARRQKMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPAVFANIRARLMASDDLIVLKPQQIDAETGKASEPAVFTTREMLRIE
YDMGRSARVLAERRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQVDAIHHITRDDGIAAVVGLAGAGKS
TLLAAARVAWEHDGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWASGRELLERGNVLVIDEAGMVS
SQQMARLLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
TEKGLDAYAQQGHLIEAGTRVETIDRIVADWSQARKQAIQNSTTAGNDGRLRGDELLVLAHTNEDVKYLN
EALRSVMVGEGALGEDRAFRTERGARAFAAGDRIIFLENARFVEPRATRLGPQHVKNGMLGTVVSTGDKR
GDAVLSVRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDDFEAKPEWGRKPHVDHAAGVTGALAETGQAKFRPNDEDADDSPYADVRTDDGTLHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVTIKVAIVDEQTGQKRFEEREVDRNVWTARRIETAEARQQRIERESHRP
DLFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGAEESLSRRWVGIAEKREQLA
KLWERASVALGFTIERERRIAYNEERAEPQTVLEADVARGRYLIPPTTGFARSAEEDARLAQLSSPAWKE
REAILRPVLETIYRDPDAALVALNALSSDTSVDPRRLADDLAKAPERLGRLRGSDLIVDGRAARDERNAA
ITALTDLLPLVRAHATQFRRNAERFESHEQQRRAHMALSIPALSEKAMARLTEIEAVRRQGGDDAYKAAF
ALAAEDRSIVQEVKAVSEALTARFGWSAFTSKADAVGERNMVERMPEDLASSRGEKLTRLFEAVKRFADE
QHLAERRDRSKIVAGASADQGMENVPMLAAVTEFRTAIDEEARSRVLSDPLYRQQRAALAAVASAIWRDP
VGAVGKIEDLLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKQAVQAVPEAAARLRSLGS
AYVNVLDAERQAIAEERRRMAVAIPALSTAAEDVLMRLTAEAKNNDRKLTASAAPLDPSIRREFAAVSRA
LDERFGRNAIIRGEKDLINRVPPAQHSAFEAMQDRLKVLQRTVRMESSEQIISERRQRAVSRGRGIEL

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1305-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1049-1127)

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 14788
• GenBank: NZ_CP125638
• Plasmid name: pRlBT01c
• Incompatibility group: -
• Plasmid size: 652849 bp
• Coordinate of oriT [Strand]: 332595..332623 [-]
• Host baterium: Rhizobium leguminosarum strain BT01

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -