Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114352
Name   oriT_pRlBT01a in_silico
Organism   Rhizobium leguminosarum strain BT01
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP125636 (462770..462798 [-], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_pRlBT01a
AGGGCGCAATATACGTCGCTGTCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9200 GenBank   WP_077991369
Name   traA_QMO81_RS26440_pRlBT01a insolico UniProt ID   _
Length   1565 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1565 a.a.        Molecular weight: 172927.47 Da        Isoelectric Point: 6.8704

>WP_077991369.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium]
MAIMFVRAQVISRGSGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELMHEELALPGQIPTWLREAIAG
QLVAKASEALWNAVDAFETRADAQLARELIIALPEELTRAENITLVREFIRDNLISKGMIADWVYHDKEG
NPHIHLMTTLRPLTEEGFGAKKVPVLGEDGKPLRVVTPDRPNGKIVYKVWAGDKETMKAWKIAWAETANR
HLALAGHDIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGRELHFAPADLARRQEMADRLLSEPELLLKQ
LGNERSTFDERDIARALHRYVDDPTDFANIRARLMASDQLVILKPQEIEAETGKVSEPAMFTTREMLRIE
YDMAQSARVLSERRGFGVSERIVTVAIERVESIESGDPKNPFRLDAEQVDAVRHVTGDGDIAAIVGLAGA
GKSTLLAAARLAWESEGHRVIGAALAGKAAEGLQDSSGIKSRTLASWELAWANGRDTLHRGDVLVIDEAG
MVASQQMARVLKIAEEAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREAWARNASRLFA
RGEVEKGLDAYARHGHLVEAGSREETIDRIVSDWAAARREAIERSTSEGGDGRLRGDELLVLAHTNDDVR
KLNEALRSVMTQEGALSESRSFRSERGVREFAAGDRIIFLENARFLEPRAKHSGPQYVKNGMLGTVVSTG
DKRGDPLLSVLLDNGRKLVFSEDSYRHVDHGYAATIHKSQGATVDRTFVLATGMMDQHLTYVSMTRHRDR
VDLYAAKEDFAAKPEWGRKPRVDHAAGVTGELVETGEAKFRPDDEDADDSPYADVRADGGTVYRLWGVSL
PKALEEAGIQEGDTVTLRKDGVERVKVQITVVDEETGHKRYEEREVDRNVWTASQVETASARQERIERES
HRPELFNPLVERLSRSGAKTTTLDFESEASYRAHANDFARRRGLDHLSLAAAEMEESLTRRWAWIAEKRE
QVEKLWERASVALGFAIERERRVAYNEERSQTMVEATSSDARTASHYVSGASADETPYLIPPATSFVSSV
EEDARLAQLASPAWTEREVILRPLLQKIYRDPDAALISLNALASDIGVAPRRLADDLAAAPGRLGRLRGS
ELIVDGRAAREERNVAVAAVKELLPMARAHATEFRRNAERFELREQTRRAHMSLSIPALSERAMARLLEI
EAVRSQGGDDAYKTAFALAAKDRSVVQEIKAVSEALTARFGWSAFSAKADVIAERNIVERMPEDLTDEWR
GKLTRLFEAVRRFADEQHLAERRDRSKIVAGASVDLRTETEKGVGKENVPIPPMLAAVTEFKVPVDDEAR
SRALASPLYRQQRAALANAATTIWRDPAEVVGKIEELLQKGFAAERIGAAVTNNPAAYGALRGSDRLMDR
MLASGRERKEAVAAVPEAAARLRALGAAHLNALDAERQAITDERRRMAVAIPALSKAAEEALAHLTVEVS
KDSRKLSVSAASLDPGIGREFAAVSRALDERFGRNALVRGDKAIANVVPPTQRGAFAAMQERLKVLQQTV
RLQSSEQIIVERRQRAASRARGINL

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1334-1425)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1072-1143)

AAA domain (AAA_30)

(394-579)

MobA/MobL family (MobA_MobL)

(17-249)


  Protein structure



No available structure.




T4CP

ID   10664 GenBank   WP_077991366
Name   traG_QMO81_RS24175_pRlBT01a insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 70297.10 Da        Isoelectric Point: 9.0817

>WP_077991366.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium]
MTPLRKPRPSLLLVLAPIAGTAFGAYVVGWRWQELAAVMSGKTEYWFLRFAPLPALLFGPLAGLLTVLAL
PLHRRRPVAIVSLVSFLAVAGFYALREYGRLAPSVESGAVSWSRALSYLDLVAVIGALMGFMAVAVAARI
STVVSEPVTRARRGIFGDAGWLSMSAAAKLFPPDGEIVVGERYRVDQEIVHELPFDPNDPATWGQGGKAS
LLTYKQDFDSTHMLFFAGSGGYKTTSNVVPTALRYSGPLICLDPSTEVAPMVAEHRAGALKREVMVLDPT
NPIMGFNVLDGIEASKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYEGRRS
LRSLRQIVSEPEPSVLAMLRDIQEHSGSAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSSDIVSGKKDVFLNISASILRSYPGIARVIIGSLINAMVQADGAFQRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNIGWDTKNNASRRSENVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDEAAK
VNRFVKPVL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(163-634)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 9338..18939

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMO81_RS24205 (QMO81_004846) 5171..5567 - 397 Protein_7 type II toxin-antitoxin system VapC family toxin -
QMO81_RS24210 (QMO81_004847) 5564..5830 - 267 WP_004676093 type II toxin-antitoxin system Phd/YefM family antitoxin -
QMO81_RS24215 (QMO81_004848) 5915..6037 - 123 WP_258719717 hypothetical protein -
QMO81_RS24220 (QMO81_004849) 6217..6476 - 260 Protein_10 toprim domain-containing protein -
QMO81_RS24225 (QMO81_004850) 6787..7977 + 1191 WP_077991364 DUF1173 domain-containing protein -
QMO81_RS24230 (QMO81_004851) 8281..8727 + 447 WP_077991362 excisionase -
QMO81_RS24235 (QMO81_004852) 8717..9157 + 441 WP_245304432 PIN domain-containing protein -
QMO81_RS24240 (QMO81_004853) 9338..10366 - 1029 WP_077991360 P-type DNA transfer ATPase VirB11 virB11
QMO81_RS24245 (QMO81_004854) 10374..11549 - 1176 WP_077991358 type IV secretion system protein VirB10 virB10
QMO81_RS24250 (QMO81_004855) 11558..12415 - 858 WP_077991357 P-type conjugative transfer protein VirB9 virB9
QMO81_RS24255 (QMO81_004856) 12412..13083 - 672 WP_077991356 virB8 family protein virB8
QMO81_RS24260 (QMO81_004857) 13085..13369 - 285 WP_077991355 hypothetical protein -
QMO81_RS24265 (QMO81_004858) 13409..14341 - 933 WP_077991353 type IV secretion system protein virB6
QMO81_RS24270 (QMO81_004859) 14344..14577 - 234 WP_007636178 EexN family lipoprotein -
QMO81_RS24275 (QMO81_004860) 14574..15275 - 702 WP_077991351 P-type DNA transfer protein VirB5 virB5
QMO81_RS24280 (QMO81_004861) 15272..17638 - 2367 WP_077991348 VirB4 family type IV secretion/conjugal transfer ATPase virb4
QMO81_RS24285 (QMO81_004862) 17631..17969 - 339 WP_077991347 type IV secretion system protein VirB3 virB3
QMO81_RS24290 (QMO81_004863) 17975..18274 - 300 WP_077991346 TrbC/VirB2 family protein virB2
QMO81_RS24295 (QMO81_004864) 18271..18939 - 669 WP_077991344 transglycosylase SLT domain-containing protein virB1
QMO81_RS24300 (QMO81_004865) 18943..19479 - 537 WP_077991342 hypothetical protein -
QMO81_RS24305 (QMO81_004866) 19578..19949 + 372 WP_004673403 hypothetical protein -
QMO81_RS24310 (QMO81_004867) 20718..20900 + 183 WP_016737443 hypothetical protein -
QMO81_RS24315 (QMO81_004868) 21011..21223 - 213 Protein_29 zincin-like metallopeptidase domain-containing protein -
QMO81_RS24320 (QMO81_004869) 21256..21807 - 552 WP_077991339 hypothetical protein -
QMO81_RS24325 (QMO81_004870) 21800..22381 - 582 WP_077991337 hypothetical protein -

Host bacterium

ID   14787 GenBank   NZ_CP125636
Plasmid name   pRlBT01a Incompatibility group   -
Plasmid size   463472 bp Coordinate of oriT [Strand]   462770..462798 [-]
Host baterium   Rhizobium leguminosarum strain BT01

Cargo genes

Drug resistance gene   -
Virulence gene   hsiC1/vipB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   nodD, nodA, nifH, nifD, nifK, nifX, mLTONO_5203, fixN, fixO, nodC, nodS, nodI, nodJ, nifT, nifZ, nifB, nifA, fixX, fixC, fixB, fixA, nifW, nifS, nifE, nifN
Anti-CRISPR   -