Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114351
Name   oriT_pRspBT03a in_silico
Organism   Rhizobium sp. BT03
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP125646 (56038..56094 [+], 57 nt)
oriT length   57 nt
IRs (inverted repeats)      24..30, 35..41  (ACGTCGC..GCGACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 57 nt

>oriT_pRspBT03a
CCCCGCCAGGGTGGCAAAACGAGACGTCGCGACAGCGACGTATAATTGCGCCCTTGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9199 GenBank   WP_283201840
Name   traA_QMO80_RS32660_pRspBT03a insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 122619.38 Da        Isoelectric Point: 9.6353

>WP_283201840.1 Ti-type conjugative transfer relaxase TraA [Rhizobium sp. BT03]
MAIAHFSVSIVSRGEGRSSVLSAAYRHCAKMDYEREARTIDYTRKQGLLHEEFALPADAPDWARTLIADR
AVSGASEAFWNRVEAFEKRTDAQLAKDLTIALPVELTAEQNIALVRDFVDKHVQSKGMVADWVYHDNPGN
PHIHLMTTLRPLAEDGFGAKKVAVTGEDGQPLKTKAGKIVYELWAGGAAEFNALRDGWFERQNHHLALNG
ISLRVDGRSYEKQGIELEPTIHLGVGAKAIERKAESQGVRPELERFKLNEARRTENTRRILRDPTAALDL
ITREKSVFNNQDIAKVLHRYVDDPSLFQQLMARILQQSEVLRLQRDTIDFATGERVPARYTTRAMIALEA
EMANRAIWLTRQTSHSVPRQVLEATFARHARLSDEQKTAIEHVAAPVRIAAIVGRAGAGKTTMMKAAREA
WEAAGYRVVGGALAGKAAEGLEMEAGIQSRTLSSWELRWQRGRHGLDARTIFVMDEAGMVASKQMAGFVE
AVVRAGAKLVLVGDPDQLQPIEAGAAFRAIADRIGYAELETIYRQREDWMRAASLDLARGNVAAALVAFG
THDRLHGSTLKADAVEQLIADWNRDYDPAKTTLMLAHLRRDVRMLNDMARATLVERGIVGDGHAFRTADG
MRQFDAGDQIVFLKNDTTLGVKNGMIGKVMQAAPNRVIVSVGEGRDQRHVAVEQRAYNNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQVYYGERSFGLNGGLEKVLSRRNAKETTLDYRNSALY
RQALSFAENRGLHIVQVARTLVHDRLDWTLRQKEKLADLTRRMRAIGERLGLFQSSIPKQKKELAPMVAG
VKQHAFSVGETIDQNLGDDPELKAQWEEVSTRFRYVFADPETAFRAMNFEAVLADGHAAKQALQKLVDEP
ASIGALNGKTGLLASKADREARRVAEINVPALKRDIEQYLTMRETARARIEGEEKTLRQRVSIDIPALSP
EARTVLERVRDAIDRNDLPAALGYAISNREAKLEIDGFNKAVSERFGERTLLTNAARNPDGPLFDKMASG
LQPAEKESLKEAWPVMRTAQQLAAHERTTATLKQAEDLRLTQRQTPTLKQ

  Protein domains


Predicted by InterproScan.

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(680-736)

AAA domain (AAA_30)

(382-568)

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(856-950)


  Protein structure



No available structure.




T4CP

ID   10662 GenBank   WP_127758573
Name   traG_QMO80_RS32675_pRspBT03a insolico UniProt ID   _
Length   642 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 642 a.a.        Molecular weight: 69350.46 Da        Isoelectric Point: 9.5877

>WP_127758573.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium/Agrobacterium group]
MSLNRLLLALLPATIMIGATIPVPGIEQWLAGFGKTAQAKLMLGRVGLALPYAIAAGAGLIFLFAANGAA
HIKAAGWSVVIGSLAVVLVAVLRETMRLLGIGASVPVGQSVLSYVDPSTAIGAAAAMLSGIFALRVAVSG
NGAFARAEPRRVRGKRAVHGDADWMSMTEAAKLFPESGGVVIGERYRVDRDSAGARSFRADSVESWGTGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTVPTSLKWGGSLVVLDPSSEVAPMVIDHRRKAGRKVIVLD
PETPAIGFNALDWIGRFGSTKEEDIVAVATWIMTDNARAPSARDDFFRASAMQLLTALIADVCLSGNTDQ
KEQTLRRVRANLSEPEPKLRERLTRIYEGSESLFVKENVAVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGNSFSTDELANGETDIFIALDLKVLEAHPGLARVVIGSFLNALYNRNGEVSGRTLFLLDEVARLGY
LRILETARDAGRKYGISLTLIFQSIGQIREAYGGRDASSKWFESASWISFAAINDPETADYLSRRCGETT
IEVDQMSRTSQSSGSSRSRSKQLSRRPLILPYEVMRMRGDEQIVFTAGNPPLRCGRAIWFRREDMKACVK
PNAFFRDTAQKQ

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(163-636)

  Protein structure



No available structure.



ID   10663 GenBank   WP_283201816
Name   t4cp2_QMO80_RS32900_pRspBT03a insolico UniProt ID   _
Length   822 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 822 a.a.        Molecular weight: 91839.46 Da        Isoelectric Point: 5.4081

>WP_283201816.1 conjugal transfer protein TrbE [Rhizobium sp. BT03]
MVALKSFRQSGPSFADLVPYAGLVDNGIMLLKDGSLMAGWYFAGPDSESSTDVEHNEVSRQINAILSRLG
SGWMIQVEAARVPTVDYPAETDCHFPDAVTRAIDAERRAHFERERGHFESRHALILTWRPPEPRRSGLTR
YVYADAGSRSATYADSALDSFRTSIREVEQYLANVVTIRRMMTEETEERGGFRIARYDELFQFIRFCVTG
ENHPVRLPEIPMYLDWLVTAELQHGLTPTVENRFLGVVAIDGLPAESWPGILNALDLMPLTYRWSSRFVF
LDEQEARARLERTRKKWQQKIRPFFDQLFQTQSRSLDQDAMMMVAESEDAIAEAASQLVSYGYYTPVIVL
FDDEQPRLQEKCEAIRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLINTRNLADLVPLNSVWS
GSPVAPCPFYPAGSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYKDAQLFA
FDKGRSMLPLTSAVGGDHYEIGGDASGEGEGSSPRLAFCPLAELSTDGDRAWATEWLETLVALQGVTVTP
DHRNAISRQIALMAQSRGRSLSDFVSGVQMREIKDALHHYSVDGPMGQLLDAEEDGLTLGAFQCFEVEEL
MNMGERNLVPVLTYLFRRVEKRLTGAPSLIILDEAWLMLGHPLFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIVDVLKESCPTKICLPNGAARELGTREFYERIGFNERQIEIVAAALPKREYYVASPDGRRLFDM
ALGPVALSFVGASGKEDLKRIRALHAEHGDTWPLLWLQQRGIAHAETYLRSP

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(191-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 98192..107832

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMO80_RS32865 (QMO80_006577) 93521..94765 - 1245 WP_127758551 plasmid replication protein RepC -
QMO80_RS32870 (QMO80_006578) 94922..95917 - 996 WP_283201812 plasmid partitioning protein RepB -
QMO80_RS32875 (QMO80_006579) 96002..97192 - 1191 WP_127758553 plasmid partitioning protein RepA -
QMO80_RS32880 (QMO80_006580) 97558..98195 + 638 Protein_96 acyl-homoserine-lactone synthase TraI -
QMO80_RS32885 (QMO80_006581) 98192..99163 + 972 WP_283201813 P-type conjugative transfer ATPase TrbB virB11
QMO80_RS32890 (QMO80_006582) 99153..99560 + 408 WP_283201814 conjugal transfer pilin TrbC virB2
QMO80_RS32895 (QMO80_006583) 99553..99852 + 300 WP_283201815 conjugal transfer protein TrbD virB3
QMO80_RS32900 (QMO80_006584) 99863..102331 + 2469 WP_283201816 conjugal transfer protein TrbE virb4
QMO80_RS32905 (QMO80_006585) 102363..103112 + 750 WP_283201850 P-type conjugative transfer protein TrbJ virB5
QMO80_RS32910 (QMO80_006586) 103109..103330 + 222 WP_283201817 entry exclusion protein TrbK -
QMO80_RS32915 (QMO80_006587) 103324..104514 + 1191 WP_283201818 P-type conjugative transfer protein TrbL virB6
QMO80_RS32920 (QMO80_006588) 104533..105195 + 663 WP_283201819 conjugal transfer protein TrbF virB8
QMO80_RS32925 (QMO80_006589) 105212..106057 + 846 WP_283201820 P-type conjugative transfer protein TrbG virB9
QMO80_RS32930 (QMO80_006590) 106057..106518 + 462 WP_283201821 conjugal transfer protein TrbH -
QMO80_RS32935 (QMO80_006591) 106531..107832 + 1302 WP_283201822 IncP-type conjugal transfer protein TrbI virB10
QMO80_RS32940 (QMO80_006592) 108493..109185 + 693 WP_127758567 response regulator transcription factor -
QMO80_RS32945 (QMO80_006593) 109242..110558 + 1317 Protein_109 transposase -
QMO80_RS32950 (QMO80_006594) 110593..111315 + 723 WP_245483072 GntR family transcriptional regulator -
QMO80_RS32955 (QMO80_006595) 111590..111851 - 262 Protein_111 transposase -

Host bacterium

ID   14786 GenBank   NZ_CP125646
Plasmid name   pRspBT03a Incompatibility group   -
Plasmid size   127889 bp Coordinate of oriT [Strand]   56038..56094 [+]
Host baterium   Rhizobium sp. BT03

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   dxnH, LinG
Symbiosis gene   -
Anti-CRISPR   -