Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114314
Name   oriT_pRA_T24_1 in_silico
Organism   Rhizobium anhuiense bv. trifolii strain T24
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP098801 (534161..534190 [+], 30 nt)
oriT length   30 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 30 nt

>oriT_pRA_T24_1
AGGGCGCAATATACGTCGCTGTCGCGACGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9168 GenBank   WP_254956080
Name   traA_NE851_RS02655_pRA_T24_1 insolico UniProt ID   _
Length   1538 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1538 a.a.        Molecular weight: 170187.93 Da        Isoelectric Point: 7.0452

>WP_254956080.1 Ti-type conjugative transfer relaxase TraA [Rhizobium anhuiense]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQTPAWLRSAIEG
RSVAGASEVLWNVVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNFTRAGMVADWVYHDKDG
NPHIHLLTPVRPLTEEGFGAKHAPILGENGEPLRFVTPGRLTGRIVYKSWGGDKETLQAWKIAWAETASR
HLALAGHDIRLDGRSYAEQGLDGIAQRHLGPEKAALSRGGAEMYFAPADLARRQEMADRLLAEPELLLKQ
LSNERSTFDERDIAKALHRYVDDPADFANIRARLMASDDLVLLKPQQVDVETGKASEPAVFTTREILRIE
YDMAQSAQVLVECRGFAVSDRAVAAAIRNVETQDPEKTFRLDPEQVDAVHHVTRDNGIAAVVGLAGAGKS
TLLAAARLAWESQGRQVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWANGQETLHRGHVVVVDEAGMVS
SQQMARVLKLVEEAGAKLVLVGDAMQLQPIQAGAAFRAITERIGFAELGGVRRQRQQWAREASRLFARGE
IGKGLDAYAQQGHLVEAETRDDVIGRIVSDWTAARKQAVQDSTAAGNDGRLRGDELLVLAHTNQDVKRLN
EELRSVMAGEGALGESRAFQTERGAREFATGDRIIFLANARFVEARAMRLGPQYVKNGMLGTVVSTGEKR
GEVLLSVRLDNGRDVVISENSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVSMTRHRHRADL
YAAKEDFEAKPEWGRKLRADHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGAVHRLWGVSLPKA
LEDAGVLEGDTVALRKDGVERVKVQVPIVDEQTGQKHFEERQVDRNIWTAKRVETAEARRERIEQESHRP
ELFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLVAAGMEEDVSRRLAWIADKRRQVA
KLWERASVALGFAIERERRVSYNEERTETHTVSETDVGGGRYLMPPATVFARSVEEEARWAQLSSPAWKE
REAILRPLLEKIYREPEAALVALNALASDTSNEPRRLADDLAIAPDRIGRLRGSDLMIDGRAARDERSIA
TAALTELLPLARAHATEFRRNAEQFESREHQRRAHMSLSIPALSRKAVARLVEIEAVRKQGGDDAYKTAF
AIAAEDRAVVQEIKAVSEALSARFGWSAFTSKADTVAERNIVERMPEDFTGTRGEKLTRLFEAVKRFAEE
QHLAERRDRSKIVASASADPQKENIPMLAAVTEFKTPIEEEARSRALAGPLYRQQRAALAETSRTIWRDP
AGAVEKIEELLVKGFAADRISAAVTNDPAAYGALRGSDRLMDRMLAAGRERRDAAQAVPEAAAWLRSLGA
AYAGALDAERQAIAEERRRMAVAIPGLSKAAEEALMWLTAEAKNDGRKRNASSAVLDPSIRREFAAVSAA
LDERFGRNAIVRGERDLINRIPPAQRRAFEAMQDRLKVLQQTVRRESSEKIMSERRQRAVSRGRGVDL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(391-578)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1047-1127)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1308-1398)

MobA/MobL family (MobA_MobL)

(17-247)


  Protein structure



No available structure.




T4CP

ID   10619 GenBank   WP_097596300
Name   traG_NE851_RS02640_pRA_T24_1 insolico UniProt ID   _
Length   637 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 637 a.a.        Molecular weight: 70260.02 Da        Isoelectric Point: 8.6032

>WP_097596300.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium]
MALKGRLHASLLLVFVPIAVTAITVYITAWRWPALAHSLSGKTEYWFFRATPVPTLLFGPLAGLLTVWAL
PLHRRRPIAIVSLLCLLVVAGFYALREYGRLAPSVESGVVTWDHALSYLDFIAVIGALAGFMAVAVAARI
SVVVPEAIKRAKGGTFGDADWLSMSAAARLFPCDGEIVVGECYRVDKEAVHQVPFDPKDRSTWGQGGQAP
LLAYSQDFDSTHMLFFAGSGGYKTTSNVLPTALRYTGPLICLDPSTEVAPMVVDHRTRMLGREVMVLDPT
NPIMGFNALDGIEHSKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPEPSVLAMLRDVQEHSETVFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFRSSDIVSGKKDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGGFSRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLLYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNVSAQCGEMTVEVQG
RSRNIGWDTKSGGARKSESVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDAQAK
ANRFVRS

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(167-634)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 124546..134143

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NE851_RS00580 (NE851_00580) 122994..123475 - 482 Protein_114 GNAT family N-acetyltransferase -
NE851_RS00585 (NE851_00585) 123559..123912 - 354 WP_097542456 MarR family transcriptional regulator -
NE851_RS00590 (NE851_00590) 124003..124542 + 540 WP_097542455 hypothetical protein -
NE851_RS00595 (NE851_00595) 124546..125208 + 663 WP_097542454 lytic transglycosylase domain-containing protein virB1
NE851_RS00600 (NE851_00600) 125205..125504 + 300 WP_097542453 TrbC/VirB2 family protein virB2
NE851_RS00605 (NE851_00605) 125510..125848 + 339 WP_097542452 type IV secretion system protein VirB3 virB3
NE851_RS00610 (NE851_00610) 125841..128207 + 2367 WP_168332964 VirB4 family type IV secretion/conjugal transfer ATPase virb4
NE851_RS00615 (NE851_00615) 128240..128905 + 666 WP_246713900 P-type DNA transfer protein VirB5 virB5
NE851_RS00620 (NE851_00620) 128902..129135 + 234 WP_254955610 EexN family lipoprotein -
NE851_RS00625 (NE851_00625) 129139..130059 + 921 WP_097622516 type IV secretion system protein virB6
NE851_RS00630 (NE851_00630) 130112..130396 + 285 WP_097595445 hypothetical protein -
NE851_RS00635 (NE851_00635) 130398..131069 + 672 WP_254956330 virB8 family protein virB8
NE851_RS00640 (NE851_00640) 131066..131923 + 858 WP_097595443 P-type conjugative transfer protein VirB9 virB9
NE851_RS00645 (NE851_00645) 131933..133105 + 1173 WP_097630250 type IV secretion system protein VirB10 virB10
NE851_RS00650 (NE851_00650) 133112..134143 + 1032 WP_184389374 P-type DNA transfer ATPase VirB11 virB11
NE851_RS00655 (NE851_00655) 134208..136106 - 1899 WP_254956332 bifunctional diguanylate cyclase/phosphodiesterase -
NE851_RS00660 (NE851_00660) 136173..136628 + 456 WP_168332970 NUDIX hydrolase -
NE851_RS00665 (NE851_00665) 136625..137647 + 1023 WP_168332971 PHB depolymerase family esterase -
NE851_RS00670 (NE851_00670) 138089..138304 - 216 WP_097595436 DUF3072 domain-containing protein -

Host bacterium

ID   14749 GenBank   NZ_CP098801
Plasmid name   pRA_T24_1 Incompatibility group   -
Plasmid size   1441560 bp Coordinate of oriT [Strand]   534161..534190 [+]
Host baterium   Rhizobium anhuiense bv. trifolii strain T24

Cargo genes

Drug resistance gene   -
Virulence gene   htpB
Metal resistance gene   actP, hupE2
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9