Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114294
Name   oriT_p12-6919.1 in_silico
Organism   Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain PNCS014868
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP039604 (8443..8531 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p12-6919.1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9161 GenBank   WP_107973980
Name   nikB_E5O12_RS23595_p12-6919.1 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104015.43 Da        Isoelectric Point: 7.3623

>WP_107973980.1 IncI1-type relaxase NikB [Salmonella enterica]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   5351 GenBank   WP_052938717
Name   WP_052938717_p12-6919.1 insolico UniProt ID   A0A706B4V0
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12643.59 Da        Isoelectric Point: 10.7463

>WP_052938717.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSTVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A706B4V0


T4CP

ID   10609 GenBank   WP_001289275
Name   trbC_E5O12_RS23590_p12-6919.1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86957.15 Da        Isoelectric Point: 6.7713

>WP_001289275.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 55194..87070

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5O12_RS23970 (E5O12_23970) 50256..51323 + 1068 WP_074182768 type IV pilus biogenesis lipoprotein PilL -
E5O12_RS23975 (E5O12_23975) 51323..51760 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
E5O12_RS23980 (E5O12_23980) 51774..53456 + 1683 WP_094307684 PilN family type IVB pilus formation outer membrane protein -
E5O12_RS23985 (E5O12_23985) 53449..54744 + 1296 WP_064148456 type 4b pilus protein PilO2 -
E5O12_RS23990 (E5O12_23990) 54731..55183 + 453 WP_001247337 type IV pilus biogenesis protein PilP -
E5O12_RS23995 (E5O12_23995) 55194..56747 + 1554 WP_136614768 GspE/PulE family protein virB11
E5O12_RS24000 (E5O12_24000) 56760..57845 + 1086 WP_063103211 type II secretion system F family protein -
E5O12_RS24005 (E5O12_24005) 57862..58476 + 615 WP_064148457 type 4 pilus major pilin -
E5O12_RS24010 (E5O12_24010) 58486..59046 + 561 WP_063124165 lytic transglycosylase domain-containing protein virB1
E5O12_RS24015 (E5O12_24015) 59031..59687 + 657 WP_074182772 A24 family peptidase -
E5O12_RS24020 (E5O12_24020) 59687..60991 + 1305 WP_064148473 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
E5O12_RS24035 (E5O12_24035) 61632..62786 + 1155 WP_001139955 tyrosine-type recombinase/integrase -
E5O12_RS24040 (E5O12_24040) 62937..63761 + 825 WP_001690630 conjugal transfer protein TraE traE
E5O12_RS24045 (E5O12_24045) 63847..65049 + 1203 WP_000976353 conjugal transfer protein TraF -
E5O12_RS24050 (E5O12_24050) 65109..65693 + 585 WP_000977525 histidine phosphatase family protein -
E5O12_RS24055 (E5O12_24055) 66088..66546 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
E5O12_RS24060 (E5O12_24060) 66543..67361 + 819 WP_032220737 IncI1-type conjugal transfer lipoprotein TraI traI
E5O12_RS24065 (E5O12_24065) 67358..68506 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
E5O12_RS24070 (E5O12_24070) 68503..68793 + 291 WP_001299214 hypothetical protein traK
E5O12_RS24075 (E5O12_24075) 68808..69359 + 552 WP_000014586 phospholipase D family protein -
E5O12_RS24080 (E5O12_24080) 69449..73213 + 3765 WP_021497833 LPD7 domain-containing protein -
E5O12_RS24085 (E5O12_24085) 73231..73578 + 348 WP_001055900 conjugal transfer protein traL
E5O12_RS24090 (E5O12_24090) 73575..74267 + 693 WP_000138551 DotI/IcmL family type IV secretion protein traM
E5O12_RS24095 (E5O12_24095) 74278..75261 + 984 WP_052938673 IncI1-type conjugal transfer protein TraN traN
E5O12_RS24100 (E5O12_24100) 75264..76553 + 1290 WP_001271991 conjugal transfer protein TraO traO
E5O12_RS24105 (E5O12_24105) 76553..77257 + 705 WP_001493811 IncI1-type conjugal transfer protein TraP traP
E5O12_RS24110 (E5O12_24110) 77257..77784 + 528 WP_001055569 conjugal transfer protein TraQ traQ
E5O12_RS24115 (E5O12_24115) 77835..78239 + 405 WP_021497835 IncI1-type conjugal transfer protein TraR traR
E5O12_RS24120 (E5O12_24120) 78303..78491 + 189 WP_001277255 putative conjugal transfer protein TraS -
E5O12_RS24125 (E5O12_24125) 78475..79275 + 801 WP_054170916 IncI1-type conjugal transfer protein TraT traT
E5O12_RS24130 (E5O12_24130) 79365..82409 + 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
E5O12_RS24135 (E5O12_24135) 82409..83023 + 615 WP_000337400 IncI1-type conjugal transfer protein TraV traV
E5O12_RS24140 (E5O12_24140) 82990..84192 + 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
E5O12_RS24145 (E5O12_24145) 84221..84805 + 585 WP_001037985 IncI1-type conjugal transfer protein TraX -
E5O12_RS24150 (E5O12_24150) 84902..87070 + 2169 WP_000698368 DotA/TraY family protein traY
E5O12_RS24155 (E5O12_24155) 87141..87803 + 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
E5O12_RS24160 (E5O12_24160) 87901..88110 + 210 WP_032178830 hemolysin expression modulator Hha -
E5O12_RS24165 (E5O12_24165) 88442..88846 - 405 WP_001175009 IS200/IS605 family transposase -
E5O12_RS24170 (E5O12_24170) 88905..90131 + 1227 WP_024186316 RNA-guided endonuclease TnpB family protein -
E5O12_RS24655 90176..90313 + 138 WP_047088497 hypothetical protein -
E5O12_RS24850 90378..90473 - 96 WP_001303310 DinQ-like type I toxin DqlB -

Host bacterium

ID   14729 GenBank   NZ_CP039604
Plasmid name   p12-6919.1 Incompatibility group   IncI
Plasmid size   90972 bp Coordinate of oriT [Strand]   8443..8531 [+]
Host baterium   Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain PNCS014868

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -