Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114276
Name   oriT_FDAARGOS_717|unnamed3 in_silico
Organism   Salmonella enterica strain FDAARGOS_717
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP054898 (47276..47364 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_FDAARGOS_717|unnamed3
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9158 GenBank   WP_001474686
Name   nikB_FOC25_RS23295_FDAARGOS_717|unnamed3 insolico UniProt ID   A0A2J0RB70
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103834.33 Da        Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A2J0RB70


Auxiliary protein

ID   5350 GenBank   WP_001283947
Name   WP_001283947_FDAARGOS_717|unnamed3 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   10606 GenBank   WP_001289271
Name   trbC_FOC25_RS23300_FDAARGOS_717|unnamed3 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86927.12 Da        Isoelectric Point: 6.7713

>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52742..90448

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC25_RS23300 (FOC25_23310) 50458..52749 - 2292 WP_001289271 F-type conjugative transfer protein TrbC -
FOC25_RS23305 (FOC25_23315) 52742..53812 - 1071 WP_000151575 IncI1-type conjugal transfer protein TrbB trbB
FOC25_RS23310 (FOC25_23320) 53831..55039 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
FOC25_RS23315 (FOC25_23325) 55331..55483 + 153 WP_001303307 Hok/Gef family protein -
FOC25_RS23320 (FOC25_23330) 55555..55806 - 252 WP_001291965 hypothetical protein -
FOC25_RS23325 (FOC25_23335) 56730..56906 - 177 WP_001054900 hypothetical protein -
FOC25_RS23330 (FOC25_23340) 57298..57507 + 210 WP_000062603 HEAT repeat domain-containing protein -
FOC25_RS23335 (FOC25_23345) 57579..58241 - 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
FOC25_RS23340 (FOC25_23350) 58312..60480 - 2169 WP_000698368 DotA/TraY family protein traY
FOC25_RS23345 (FOC25_23355) 60577..61161 - 585 WP_001037998 conjugal transfer protein TraX -
FOC25_RS23350 (FOC25_23360) 61190..62392 - 1203 WP_001189158 IncI1-type conjugal transfer protein TraW traW
FOC25_RS23355 (FOC25_23365) 62359..62973 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
FOC25_RS23360 (FOC25_23370) 62973..66017 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
FOC25_RS23365 (FOC25_23375) 66107..66907 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
FOC25_RS23370 (FOC25_23380) 66891..67079 - 189 WP_001277255 putative conjugal transfer protein TraS -
FOC25_RS23375 (FOC25_23385) 67143..67547 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
FOC25_RS23380 (FOC25_23390) 67598..68125 - 528 WP_001055569 conjugal transfer protein TraQ traQ
FOC25_RS23385 (FOC25_23395) 68125..68829 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
FOC25_RS23390 (FOC25_23400) 68829..70118 - 1290 WP_001271997 conjugal transfer protein TraO traO
FOC25_RS23395 (FOC25_23405) 70121..71104 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
FOC25_RS23400 (FOC25_23410) 71115..71807 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
FOC25_RS23405 (FOC25_23415) 71804..72151 - 348 WP_001055900 conjugal transfer protein traL
FOC25_RS23410 (FOC25_23420) 72169..75936 - 3768 WP_001141535 LPD7 domain-containing protein -
FOC25_RS23415 (FOC25_23425) 76026..76577 - 552 WP_000014584 phospholipase D family protein -
FOC25_RS23420 (FOC25_23430) 76592..76882 - 291 WP_001299214 hypothetical protein traK
FOC25_RS23425 (FOC25_23435) 76879..78027 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
FOC25_RS23430 (FOC25_23440) 78024..78842 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
FOC25_RS23435 (FOC25_23445) 78839..79297 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
FOC25_RS23440 (FOC25_23450) 79692..80276 - 585 WP_000977517 histidine phosphatase family protein -
FOC25_RS23445 (FOC25_23455) 80336..81538 - 1203 WP_000976353 conjugal transfer protein TraF -
FOC25_RS23450 (FOC25_23460) 81624..82448 - 825 WP_001238927 conjugal transfer protein TraE traE
FOC25_RS23455 (FOC25_23465) 82599..83753 - 1155 WP_001139958 tyrosine-type recombinase/integrase -
FOC25_RS24310 (FOC25_23470) 84339..84560 + 222 Protein_93 prepilin -
FOC25_RS23465 (FOC25_23475) 84686..85942 - 1257 WP_254879437 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
FOC25_RS23470 (FOC25_23480) 85942..86598 - 657 WP_001193553 prepilin peptidase -
FOC25_RS23475 (FOC25_23485) 86583..87143 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
FOC25_RS23480 (FOC25_23490) 87153..87767 - 615 WP_000959785 type 4 pilus major pilin -
FOC25_RS23485 (FOC25_23495) 87785..88882 - 1098 WP_001208805 type II secretion system F family protein -
FOC25_RS23490 (FOC25_23500) 88895..90448 - 1554 WP_000362202 GspE/PulE family protein virB11
FOC25_RS23495 (FOC25_23505) 90459..90911 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
FOC25_RS23500 (FOC25_23510) 90898..92193 - 1296 WP_000752774 type 4b pilus protein PilO2 -
FOC25_RS23505 (FOC25_23515) 92186..93868 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
FOC25_RS23510 (FOC25_23520) 93882..94319 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
FOC25_RS23515 (FOC25_23525) 94319..95386 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   14711 GenBank   NZ_CP054898
Plasmid name   FDAARGOS_717|unnamed3 Incompatibility group   IncI1
Plasmid size   146288 bp Coordinate of oriT [Strand]   47276..47364 [-]
Host baterium   Salmonella enterica strain FDAARGOS_717

Cargo genes

Drug resistance gene   aadA2, cmlA1, ant(3'')-Ia, sul3, tet(A), blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -