Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114264
Name   oriT_SA25-SX|unnamed1 in_silico
Organism   Staphylococcus aureus strain SA25-SX
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP130519 (10950..11189 [-], 240 nt)
oriT length   240 nt
IRs (inverted repeats)      217..222, 232..237  (ATTTTA..TAAAAT)
 171..178, 183..190  (CTATCATT..AATGATAG)
 154..160, 164..170  (GTCTGGC..GCCAGAC)
 26..32, 44..50  (TTTTTTA..TAAAAAA)
 37..42, 45..50  (TTTTTT..AAAAAA)
 1..7, 20..26  (AAGACAT..ATGTCTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 240 nt

>oriT_SA25-SX|unnamed1
AAGACATTAGTGATAACTGATGTCTTTTTTTATTGATTTTTTGTAAAAAAGTACTGTCTTAGTTTTGTGACAAATACTGTATATAGTGTCACAAAAGTGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTTTTGAATGATAGCAAATTCCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9156 GenBank   WP_000122374
Name   mobV_Q3V56_RS14040_SA25-SX|unnamed1 insolico UniProt ID   A0A2R4A9A7
Length   413 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 413 a.a.        Molecular weight: 48526.00 Da        Isoelectric Point: 6.1679

>WP_000122374.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGK
RKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVP
ITDDGRLSAKEVVGNKKALTAFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERE
SQKTDHIKQKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKA
AQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIEIALKLLKILL
KELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQEQQKNQKRDRGMHL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-198)


  Protein structure


Source ID Structure
AlphaFold DB A0A2R4A9A7


Host bacterium

ID   14699 GenBank   NZ_CP130519
Plasmid name   SA25-SX|unnamed1 Incompatibility group   -
Plasmid size   25958 bp Coordinate of oriT [Strand]   10950..11189 [-]
Host baterium   Staphylococcus aureus strain SA25-SX

Cargo genes

Drug resistance gene   blaZ, tet(K)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21