Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114205 |
| Name | oriT_pCFSA244-2  |
| Organism | Salmonella enterica subsp. enterica strain CFSA244 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP033254 (13893..13945 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pCFSA244-2
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10565 | GenBank | WP_015059539 |
| Name | t4cp2_EB533_RS02230_pCFSA244-2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32494..55324
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EB533_RS02150 (EB533_00990) | 27628..28281 | - | 654 | WP_170855322 | hypothetical protein | - |
| EB533_RS02155 (EB533_00995) | 28293..29417 | - | 1125 | WP_000486716 | site-specific integrase | - |
| EB533_RS27200 (EB533_01015) | 30331..30549 | - | 219 | Protein_34 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| EB533_RS02180 (EB533_01020) | 30568..31842 | - | 1275 | WP_235883108 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| EB533_RS02185 (EB533_01025) | 31855..32490 | - | 636 | WP_000934977 | A24 family peptidase | - |
| EB533_RS02190 (EB533_01030) | 32494..32976 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| EB533_RS02195 (EB533_01035) | 33042..33605 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| EB533_RS02200 (EB533_01040) | 33655..34764 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| EB533_RS02205 (EB533_01045) | 34755..36293 | - | 1539 | WP_000466225 | GspE/PulE family protein | virB11 |
| EB533_RS02210 (EB533_01050) | 36318..36812 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| EB533_RS02215 (EB533_01055) | 36796..38106 | - | 1311 | WP_254701936 | type 4b pilus protein PilO2 | - |
| EB533_RS02220 (EB533_01060) | 38157..39800 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| EB533_RS02225 (EB533_01065) | 39793..40329 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| EB533_RS02230 (EB533_01070) | 40376..42334 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| EB533_RS02235 (EB533_01075) | 42350..43405 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| EB533_RS02240 (EB533_01080) | 43424..44563 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| EB533_RS02245 (EB533_01085) | 44553..45254 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| EB533_RS02250 (EB533_01090) | 45320..46054 | - | 735 | WP_000432282 | virB8 family protein | virB8 |
| EB533_RS02255 (EB533_01095) | 46054..46188 | - | 135 | WP_000701233 | hypothetical protein | - |
| EB533_RS02260 (EB533_01100) | 46220..48577 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| EB533_RS02265 (EB533_01105) | 48583..48903 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| EB533_RS26940 | 48974..49264 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| EB533_RS02275 (EB533_01115) | 49264..49848 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| EB533_RS02280 (EB533_01120) | 49869..50267 | - | 399 | WP_001153669 | hypothetical protein | - |
| EB533_RS02285 (EB533_01125) | 50386..50823 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| EB533_RS02290 (EB533_01130) | 50829..52064 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| EB533_RS02295 (EB533_01135) | 52067..52366 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| EB533_RS02300 (EB533_01140) | 52414..53223 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| EB533_RS02305 (EB533_01145) | 53446..53670 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| EB533_RS02310 (EB533_01150) | 53679..54323 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| EB533_RS02315 (EB533_01155) | 54329..55324 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| EB533_RS02320 (EB533_01160) | 55328..55585 | - | 258 | WP_000739144 | hypothetical protein | - |
| EB533_RS02325 (EB533_01165) | 55582..55884 | - | 303 | WP_001360345 | hypothetical protein | - |
| EB533_RS02330 (EB533_01170) | 55865..56122 | - | 258 | WP_001542015 | hypothetical protein | - |
| EB533_RS02335 (EB533_01175) | 56155..56601 | - | 447 | WP_001243165 | hypothetical protein | - |
| EB533_RS26750 | 56612..56782 | - | 171 | WP_000550720 | hypothetical protein | - |
| EB533_RS02340 (EB533_01180) | 56786..57229 | - | 444 | WP_000964330 | NfeD family protein | - |
| EB533_RS02350 (EB533_01190) | 57603..58556 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| EB533_RS26755 | 58583..58759 | - | 177 | WP_000753050 | hypothetical protein | - |
| EB533_RS02355 (EB533_01195) | 58752..58967 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| EB533_RS02360 (EB533_01200) | 58960..59412 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 14640 | GenBank | NZ_CP033254 |
| Plasmid name | pCFSA244-2 | Incompatibility group | - |
| Plasmid size | 60381 bp | Coordinate of oriT [Strand] | 13893..13945 [-] |
| Host baterium | Salmonella enterica subsp. enterica strain CFSA244 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |