Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114198 |
| Name | oriT_pCFSA664-2  |
| Organism | Salmonella enterica subsp. enterica strain CFSA664 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP033354 (12090..12192 [-], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 65..70, 73..78 (CACCGT..ACGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_pCFSA664-2
AGCCTCGCAGAGCAGGACTCCCGTTGAGCAGCCCCGCTGCGAATAAGGGAAGGTAAAGTAGAAGCACCGTTTACGGTGGGCCTACTTTACATGTCCTGCCCGG
AGCCTCGCAGAGCAGGACTCCCGTTGAGCAGCCCCGCTGCGAATAAGGGAAGGTAAAGTAGAAGCACCGTTTACGGTGGGCCTACTTTACATGTCCTGCCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9117 | GenBank | WP_023352245 |
| Name | traI_EB780_RS03725_pCFSA664-2 |
UniProt ID | _ |
| Length | 877 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 877 a.a. Molecular weight: 97961.42 Da Isoelectric Point: 10.5942
>WP_023352245.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MISRHIPMRNVKKSSFFRLAEYLQDSQNKQERVGAVKTTNCHQENILDAARYEIEPTQRLNTRAKGDKTY
HLVISFRPGENPPQEVLDVIEERMCTALGYGEHQRVSAVHYDTDNVHIQIAINKVHPKTHNIHTPYNDYK
IRTAVCQELEKEFGLEKDNHRASKTRSENNAADMERHSGRESLLGWIKRECADQLKSAANWQELHKAMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRLSPEAIKQRFGSAVHV
KRPGERPPPIRENRQVHLGEIPVMSINNGKRYEGKPLGSQGASTSVLYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRTGRLKRAAIKLLKGPGVNKRHLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKILRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFTDPELEKLRQILAKK
AAREREYIHIPFEQRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTEA
GRERSGNGNATGANEPGVTGRSPSADAGNGYGTSNSGRRTGSGDGVTTAGSQPGRSDDTRTGRSRAAESN
GERGHRKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLPGNVPDQLEHKTAERTDQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR
MISRHIPMRNVKKSSFFRLAEYLQDSQNKQERVGAVKTTNCHQENILDAARYEIEPTQRLNTRAKGDKTY
HLVISFRPGENPPQEVLDVIEERMCTALGYGEHQRVSAVHYDTDNVHIQIAINKVHPKTHNIHTPYNDYK
IRTAVCQELEKEFGLEKDNHRASKTRSENNAADMERHSGRESLLGWIKRECADQLKSAANWQELHKAMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRLSPEAIKQRFGSAVHV
KRPGERPPPIRENRQVHLGEIPVMSINNGKRYEGKPLGSQGASTSVLYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRTGRLKRAAIKLLKGPGVNKRHLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKILRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFTDPELEKLRQILAKK
AAREREYIHIPFEQRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTEA
GRERSGNGNATGANEPGVTGRSPSADAGNGYGTSNSGRRTGSGDGVTTAGSQPGRSDDTRTGRSRAAESN
GERGHRKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLPGNVPDQLEHKTAERTDQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 10562 | GenBank | WP_001231127 |
| Name | t4cp2_EB780_RS03735_pCFSA664-2 |
UniProt ID | _ |
| Length | 647 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 647 a.a. Molecular weight: 71661.84 Da Isoelectric Point: 8.3842
>WP_001231127.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKAAKGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHERLPDAFFAAGSVGAAVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIRDAGLLNDEGVY
VGAWEDKNGQLRYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYAK
NLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVLY
KHKNEGTPATLPYVDSIMADPERGAGELWMEMTQYSHINGENHPVVGAAGRDMMDRPEEEAGSVLSTAKS
YLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERVD
NNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLGMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQDI
NQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTIQ
EVQRPLLTVDECLRMPGPKKNAEGLITERGDMVIYVAGFPAIYGKQPLFFQDEIFSLRASVPAPKVTDRI
RAVAAANDDASNEAIAI
MRKPNNAVGPQVRNKKKAAKGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHERLPDAFFAAGSVGAAVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIRDAGLLNDEGVY
VGAWEDKNGQLRYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYAK
NLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVLY
KHKNEGTPATLPYVDSIMADPERGAGELWMEMTQYSHINGENHPVVGAAGRDMMDRPEEEAGSVLSTAKS
YLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERVD
NNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLGMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQDI
NQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTIQ
EVQRPLLTVDECLRMPGPKKNAEGLITERGDMVIYVAGFPAIYGKQPLFFQDEIFSLRASVPAPKVTDRI
RAVAAANDDASNEAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 31094..41319
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EB780_RS27430 | 26390..26596 | - | 207 | WP_000087003 | hypothetical protein | - |
| EB780_RS03785 (EB780_01680) | 26875..27072 | + | 198 | WP_023352252 | hypothetical protein | - |
| EB780_RS03790 (EB780_01685) | 27243..28130 | - | 888 | WP_225088985 | hypothetical protein | - |
| EB780_RS03795 (EB780_01690) | 28358..28723 | + | 366 | WP_023352253 | hypothetical protein | - |
| EB780_RS03800 (EB780_01695) | 28807..29421 | + | 615 | WP_021519995 | recombinase family protein | - |
| EB780_RS03805 (EB780_01700) | 29453..29821 | + | 369 | WP_023352254 | helix-turn-helix transcriptional regulator | - |
| EB780_RS03810 (EB780_01705) | 29986..30759 | - | 774 | WP_001146459 | transglycosylase SLT domain-containing protein | - |
| EB780_RS03815 (EB780_26145) | 30775..31083 | - | 309 | WP_023146507 | hypothetical protein | - |
| EB780_RS03820 (EB780_01710) | 31094..32638 | - | 1545 | WP_024187738 | P-type conjugative transfer protein TrbL | virB6 |
| EB780_RS03825 (EB780_01715) | 32643..32816 | - | 174 | WP_019842051 | entry exclusion lipoprotein TrbK | - |
| EB780_RS03830 (EB780_01720) | 32829..33599 | - | 771 | WP_023146486 | P-type conjugative transfer protein TrbJ | virB5 |
| EB780_RS03835 (EB780_01725) | 33612..35006 | - | 1395 | WP_031626058 | TrbI/VirB10 family protein | virB10 |
| EB780_RS03840 (EB780_01730) | 35010..35453 | - | 444 | WP_000642284 | hypothetical protein | - |
| EB780_RS03845 (EB780_01735) | 35456..36346 | - | 891 | WP_000748212 | P-type conjugative transfer protein TrbG | virB9 |
| EB780_RS03850 (EB780_01740) | 36362..37069 | - | 708 | WP_000765386 | VirB8/TrbF family protein | virB8 |
| EB780_RS03855 (EB780_01745) | 37066..39609 | - | 2544 | WP_001090276 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| EB780_RS03860 (EB780_01750) | 39606..39932 | - | 327 | WP_001208102 | conjugal transfer protein TrbD | virB3 |
| EB780_RS03865 (EB780_01755) | 39935..40318 | - | 384 | WP_023352257 | TrbC/VirB2 family protein | virB2 |
| EB780_RS03870 (EB780_01760) | 40432..41319 | - | 888 | WP_019842083 | P-type conjugative transfer ATPase TrbB | virB11 |
Host bacterium
| ID | 14633 | GenBank | NZ_CP033354 |
| Plasmid name | pCFSA664-2 | Incompatibility group | IncP1 |
| Plasmid size | 41696 bp | Coordinate of oriT [Strand] | 12090..12192 [-] |
| Host baterium | Salmonella enterica subsp. enterica strain CFSA664 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |