Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114188
Name   oriT_p5435N in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain NMI5435/13
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ111278 (11393..11482 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_p5435N
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9111 GenBank   WP_024561907
Name   mobF_Q5M28_RS00105_p5435N insolico UniProt ID   H1ZMV6
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118896.59 Da        Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure


Source ID Structure
AlphaFold DB H1ZMV6


T4CP

ID   10557 GenBank   WP_032493179
Name   t4cp2_Q5M28_RS00100_p5435N insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57912.96 Da        Isoelectric Point: 9.5127

>WP_032493179.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERDHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12053..32156

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q5M28_RS00055 7168..7368 + 201 WP_015060066 hypothetical protein -
Q5M28_RS00060 7423..7920 + 498 WP_040115102 MucR family transcriptional regulator -
Q5M28_RS00065 8700..8906 + 207 WP_032493183 DUF905 family protein -
Q5M28_RS00070 8981..9163 + 183 WP_032493182 hypothetical protein -
Q5M28_RS00075 9222..9572 - 351 WP_014014961 hypothetical protein -
Q5M28_RS00080 9634..10002 - 369 WP_000360828 plasmid stabilization protein StbC -
Q5M28_RS00085 10005..10721 - 717 WP_000861579 StbB family protein -
Q5M28_RS00090 10730..11161 - 432 WP_029697170 plasmid stabilization protein StbA -
Q5M28_RS00095 11577..12053 + 477 WP_001238978 hypothetical protein -
Q5M28_RS00100 12053..13585 + 1533 WP_032493179 type IV secretion system DNA-binding domain-containing protein virb4
Q5M28_RS00105 13590..16808 + 3219 WP_024561907 MobF family relaxase -
Q5M28_RS00110 16805..17470 + 666 WP_000971716 DUF6710 family protein -
Q5M28_RS00115 17463..17798 + 336 WP_058677743 hypothetical protein -
Q5M28_RS00120 17858..18061 - 204 WP_001567330 hypothetical protein -
Q5M28_RS00125 18104..18511 - 408 WP_001567331 hypothetical protein -
Q5M28_RS00130 18583..19125 - 543 WP_001567332 hypothetical protein -
Q5M28_RS00135 19109..19516 - 408 WP_172685526 hypothetical protein -
Q5M28_RS00140 19569..20108 - 540 WP_001567334 hypothetical protein -
Q5M28_RS00145 20324..20725 - 402 WP_302615268 hypothetical protein -
Q5M28_RS00150 21356..21868 - 513 WP_029697418 phospholipase D family protein -
Q5M28_RS00155 21880..22881 - 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
Q5M28_RS00160 22932..24065 - 1134 WP_000101920 type IV secretion system protein VirB10 virB10
Q5M28_RS00165 24065..24952 - 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
Q5M28_RS00170 24963..25658 - 696 WP_001208352 type IV secretion system protein virB8
Q5M28_RS00315 25669..25800 - 132 WP_032493177 hypothetical protein -
Q5M28_RS00175 25877..26914 - 1038 WP_014014948 type IV secretion system protein virB6
Q5M28_RS00180 26931..27173 - 243 WP_000858958 EexN family lipoprotein -
Q5M28_RS00185 27183..27884 - 702 WP_014014949 type IV secretion system protein virB5
Q5M28_RS00190 27900..30473 - 2574 WP_032493176 VirB4 family type IV secretion/conjugal transfer ATPase virb4
Q5M28_RS00195 30474..30791 - 318 WP_000462629 type IV secretion system protein VirB3 virB3
Q5M28_RS00200 30831..31172 - 342 WP_001121032 TrbC/VirB2 family protein virB2
Q5M28_RS00205 31374..32156 - 783 WP_024562119 lytic transglycosylase domain-containing protein virB1
Q5M28_RS00210 32220..32573 + 354 WP_225988093 H-NS family nucleoid-associated regulatory protein -
Q5M28_RS00215 32577..32927 + 351 WP_024562120 hypothetical protein -
Q5M28_RS00220 32906..33262 + 357 WP_227648693 hypothetical protein -
Q5M28_RS00225 33266..33574 + 309 WP_000733764 hypothetical protein -
Q5M28_RS00230 33859..34044 + 186 WP_024562121 ribbon-helix-helix domain-containing protein -
Q5M28_RS00235 34041..34649 - 609 WP_000275218 recombinase family protein -
Q5M28_RS00240 35010..35939 - 930 Protein_48 class 1 integron integrase IntI1 -
Q5M28_RS00245 36098..36652 + 555 WP_032488579 aminoglycoside N-acetyltransferase AAC(6')-Ib3 -

Host bacterium

ID   14623 GenBank   NZ_OQ111278
Plasmid name   p5435N Incompatibility group   IncN3
Plasmid size   46561 bp Coordinate of oriT [Strand]   11393..11482 [-]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain NMI5435/13

Cargo genes

Drug resistance gene   aac(6')-Ib-cr, blaVIM-4, qacE, sul1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -