Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114179 |
| Name | oriT_p14-SA00836-0  |
| Organism | Salmonella enterica subsp. diarizonae serovar 61:k:15(7) strain 14-SA00836-0 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP054423 (38424..39030 [+], 607 nt) |
| oriT length | 607 nt |
| IRs (inverted repeats) | 590..595, 600..605 (ATGCCC..GGGCAT) 517..523, 529..535 (CCTCCCG..CGGGAGG) 453..458, 462..467 (GTTCGC..GCGAAC) 196..201, 205..210 (TCAATT..AATTGA) 175..180, 187..192 (AAATCA..TGATTT) 103..108, 120..125 (GCTAAA..TTTAGC) 79..84, 93..98 (GCTTTA..TAAAGC) 29..37, 46..54 (TTTTGATAA..TTATCAAAA) |
| Location of nic site | 313..314 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 607 nt
>oriT_p14-SA00836-0
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATACCCTTACTGCCAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTAAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATACCCTTACTGCCAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTAAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9104 | GenBank | WP_064055706 |
| Name | mobP1_HTZ89_RS23190_p14-SA00836-0 |
UniProt ID | _ |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44346.72 Da Isoelectric Point: 10.5368
>WP_064055706.1 MobP1 family relaxase [Escherichia coli]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 10550 | GenBank | WP_064055712 |
| Name | t4cp2_HTZ89_RS23130_p14-SA00836-0 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69461.40 Da Isoelectric Point: 7.1383
>WP_064055712.1 type IV secretory system conjugative DNA transfer family protein [Salmonella enterica]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVLSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFCVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVLSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFCVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 25929..35884
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTZ89_RS23110 (HTZ89_23110) | 20939..21145 | - | 207 | WP_000850859 | hemolysin expression modulator Hha | - |
| HTZ89_RS23115 (HTZ89_23115) | 21142..23295 | - | 2154 | WP_064055713 | type IA DNA topoisomerase | - |
| HTZ89_RS23120 (HTZ89_23120) | 23382..23684 | - | 303 | WP_024242858 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTZ89_RS23125 (HTZ89_23125) | 23681..24094 | - | 414 | WP_000722606 | cag pathogenicity island Cag12 family protein | - |
| HTZ89_RS23130 (HTZ89_23130) | 24091..25926 | - | 1836 | WP_064055712 | type IV secretory system conjugative DNA transfer family protein | - |
| HTZ89_RS23135 (HTZ89_23135) | 25929..26960 | - | 1032 | WP_064055711 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTZ89_RS23140 (HTZ89_23140) | 26962..28167 | - | 1206 | WP_064055710 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| HTZ89_RS23145 (HTZ89_23145) | 28164..29094 | - | 931 | Protein_30 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTZ89_RS23150 (HTZ89_23150) | 29099..29812 | - | 714 | WP_000394612 | type IV secretion system protein | virB8 |
| HTZ89_RS23595 | 29802..29930 | - | 129 | WP_001328099 | hypothetical protein | - |
| HTZ89_RS23155 (HTZ89_23155) | 30059..31120 | - | 1062 | WP_064055709 | type IV secretion system protein | virB6 |
| HTZ89_RS23160 (HTZ89_23160) | 31132..31416 | - | 285 | WP_064055708 | EexN family lipoprotein | - |
| HTZ89_RS23165 (HTZ89_23165) | 31431..32180 | - | 750 | WP_064055707 | type IV secretion system protein | - |
| HTZ89_RS23170 (HTZ89_23170) | 32191..34944 | - | 2754 | WP_001328100 | VirB3 family type IV secretion system protein | virb4 |
| HTZ89_RS23175 (HTZ89_23175) | 34963..35259 | - | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| HTZ89_RS23180 (HTZ89_23180) | 35237..35884 | - | 648 | WP_000953529 | lytic transglycosylase domain-containing protein | virB1 |
| HTZ89_RS23185 (HTZ89_23185) | 36059..36577 | - | 519 | WP_001446885 | transcription termination/antitermination NusG family protein | - |
| HTZ89_RS23190 (HTZ89_23190) | 36930..38096 | - | 1167 | WP_064055706 | MobP1 family relaxase | - |
| HTZ89_RS23195 (HTZ89_23195) | 38099..38644 | - | 546 | WP_000757692 | DNA distortion polypeptide 1 | - |
| HTZ89_RS23205 (HTZ89_23205) | 38970..39242 | - | 273 | WP_000160399 | hypothetical protein | - |
| HTZ89_RS23210 (HTZ89_23210) | 39255..39404 | - | 150 | WP_000003880 | hypothetical protein | - |
| HTZ89_RS23215 (HTZ89_23215) | 39474..39653 | - | 180 | WP_000439078 | hypothetical protein | - |
| HTZ89_RS23220 (HTZ89_23220) | 39696..40025 | - | 330 | WP_000866648 | hypothetical protein | - |
| HTZ89_RS23225 (HTZ89_23225) | 40118..40333 | - | 216 | WP_001180116 | hypothetical protein | - |
| HTZ89_RS23230 (HTZ89_23230) | 40323..40568 | - | 246 | WP_000356546 | hypothetical protein | - |
Host bacterium
| ID | 14614 | GenBank | NZ_CP054423 |
| Plasmid name | p14-SA00836-0 | Incompatibility group | IncX1 |
| Plasmid size | 42668 bp | Coordinate of oriT [Strand] | 38424..39030 [+] |
| Host baterium | Salmonella enterica subsp. diarizonae serovar 61:k:15(7) strain 14-SA00836-0 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIF7 |