Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114177
Name   oriT_p1_EfsPF24-2_repUS11 in_silico
Organism   Enterococcus faecalis strain EfsPF24-2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP124932 (26066..26115 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_p1_EfsPF24-2_repUS11
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9102 GenBank   WP_002367932
Name   Relaxase_QLQ44_RS14515_p1_EfsPF24-2_repUS11 insolico UniProt ID   R3K157
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65400.15 Da        Isoelectric Point: 6.0174

>WP_002367932.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure


Source ID Structure
AlphaFold DB R3K157


Auxiliary protein

ID   5314 GenBank   WP_002367696
Name   WP_002367696_p1_EfsPF24-2_repUS11 insolico UniProt ID   Q82YL4
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 14042.10 Da        Isoelectric Point: 9.9724

>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure


Source ID Structure
AlphaFold DB Q82YL4


T4CP

ID   10548 GenBank   WP_010815743
Name   t4cp2_QLQ44_RS14535_p1_EfsPF24-2_repUS11 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70159.16 Da        Isoelectric Point: 6.1803

>WP_010815743.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNDAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 28760..50306

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLQ44_RS14515 (QLQ44_14510) 23957..25642 - 1686 WP_002367932 relaxase/mobilization nuclease domain-containing protein -
QLQ44_RS14520 (QLQ44_14515) 25643..25999 - 357 WP_002367696 plasmid mobilization relaxosome protein MobC -
QLQ44_RS14525 (QLQ44_14520) 26245..26517 - 273 WP_002362409 LtrD -
QLQ44_RS14530 (QLQ44_14525) 26550..28709 - 2160 WP_010815744 ArdC-like ssDNA-binding domain-containing protein -
QLQ44_RS14535 (QLQ44_14530) 28760..30589 - 1830 WP_010815743 VirD4-like conjugal transfer protein, CD1115 family virb4
QLQ44_RS14540 (QLQ44_14535) 30589..31074 - 486 WP_002386493 PcfB family protein gbs1365
QLQ44_RS14545 (QLQ44_14540) 31116..31298 - 183 WP_002367689 hypothetical protein -
QLQ44_RS14550 (QLQ44_14545) 31344..31802 - 459 WP_002382810 single-stranded DNA-binding protein -
QLQ44_RS14555 (QLQ44_14550) 31819..32574 - 756 WP_002415346 Fic family protein -
QLQ44_RS14560 (QLQ44_14555) 32688..33296 - 609 WP_010815741 hypothetical protein -
QLQ44_RS14565 (QLQ44_14560) 33280..33525 - 246 WP_002410289 hypothetical protein -
QLQ44_RS14570 (QLQ44_14565) 33512..34138 - 627 WP_002386490 hypothetical protein prgL
QLQ44_RS14575 (QLQ44_14570) 34411..36345 - 1935 WP_002367922 group II intron reverse transcriptase/maturase -
QLQ44_RS14580 (QLQ44_14575) 37069..39582 - 2514 WP_010815739 phage tail tip lysozyme prgK
QLQ44_RS14585 (QLQ44_14580) 39594..41984 - 2391 WP_174096038 VirB4-like conjugal transfer ATPase, CD1110 family virb4
QLQ44_RS14590 (QLQ44_14585) 41938..42291 - 354 WP_002386485 PrgI family protein prgIc
QLQ44_RS14595 (QLQ44_14590) 42293..43093 - 801 WP_002382799 type IV secretion system protein prgHb
QLQ44_RS14600 (QLQ44_14595) 43108..43404 - 297 WP_002382798 hypothetical protein -
QLQ44_RS14605 (QLQ44_14600) 43417..43644 - 228 WP_002365858 hypothetical protein -
QLQ44_RS14610 (QLQ44_14605) 43691..44125 - 435 WP_002365857 hypothetical protein -
QLQ44_RS14615 (QLQ44_14610) 44145..45044 - 900 WP_002409287 hypothetical protein -
QLQ44_RS14620 (QLQ44_14615) 45086..45943 - 858 WP_002367673 pheromone response LPXTG-anchored adhesin PrgC prgC
QLQ44_RS14625 (QLQ44_14620) 45971..46327 - 357 WP_002367672 pheromone response system RNA-binding regulator PrgU -
QLQ44_RS14630 (QLQ44_14625) 46389..50306 - 3918 WP_142960233 LPXTG-anchored aggregation substance PrgB/Asc10 prgB
QLQ44_RS14635 (QLQ44_14630) 50518..50706 - 189 Protein_57 hypothetical protein -
QLQ44_RS14640 (QLQ44_14635) 50978..51172 - 195 WP_002415094 hypothetical protein -
QLQ44_RS14645 (QLQ44_14640) 51251..53923 - 2673 WP_284452411 surface exclusion protein SEA1/PrgA -
QLQ44_RS14650 (QLQ44_14645) 53934..54239 - 306 WP_049098694 hypothetical protein -
QLQ44_RS14655 (QLQ44_14650) 54809..55189 - 381 WP_002367664 hypothetical protein -

Host bacterium

ID   14612 GenBank   NZ_CP124932
Plasmid name   p1_EfsPF24-2_repUS11 Incompatibility group   -
Plasmid size   74019 bp Coordinate of oriT [Strand]   26066..26115 [-]
Host baterium   Enterococcus faecalis strain EfsPF24-2

Cargo genes

Drug resistance gene   -
Virulence gene   prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9