Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114163
• Name: oriT_HAF242|unnamed1
• Organism: Staphylococcus epidermidis strain HAF242
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP090942 (50103..50180 [+], 78 nt)
• oriT length: 78 nt
• IRs (inverted repeats): 53..60, 62..69 (TTGGGGAT..ATCCCCAA) 22..29, 34..41 (ATTTTTTC..GAAAAAAT) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 78 nt

>oriT_HAF242|unnamed1
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGATAATCCCCAACAAGCTGGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobL_LZT96_RS12200_HAF242|unnamed1

• ID: 9094
• GenBank: WP_002456271
• Name: mobL_LZT96_RS12200_HAF242|unnamed1
• UniProt ID: _
• Length: 273 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 273 a.a.
• Molecular weight: 32676.63 Da
• Isoelectric Point: 10.2967

>WP_002456271.1 MULTISPECIES: relaxase MobL [Bacillota]
MSEKTSPAINLICDFKVGSSKGESYKKFINYISTEKNKENVKKQDIEGNLFDKYLDYMSIDKLKKKQGYN
KENVKKRTTMSFNSEHEFIEEQEMNRLKDKFQEANKKSHVMYQDVVSFDNQFLKDNHLFNESLDKLDEPK
IKKATRRMVNQMIKDNKMDENHTFWCANIHYDTDNIHIHIATSEEKNTRKTIKEGRFKGQYRGKRTPTTL
RNMKSTFANSLYKDINLMKEIHQLKKEMRKEVKINLSRECKDWTASLNIKMKKLKKEMKRIRK

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (7-266)

Protein structure

No available structure.

Host bacterium

• ID: 14598
• GenBank: NZ_CP090942
• Plasmid name: HAF242|unnamed1
• Incompatibility group: -
• Plasmid size: 64388 bp
• Coordinate of oriT [Strand]: 50103..50180 [+]
• Host baterium: Staphylococcus epidermidis strain HAF242

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21