Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114099 |
| Name | oriT_pR18.1297_62k  |
| Organism | Salmonella enterica subsp. enterica serovar Goldcoast strain R18.1297 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP100687 (12803..12855 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pR18.1297_62k
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10478 | GenBank | WP_254521165 |
| Name | t4cp2_FC640_RS23950_pR18.1297_62k |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73584.31 Da Isoelectric Point: 9.5070
>WP_254521165.1 type IV secretory system conjugative DNA transfer family protein [Salmonella enterica]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKTKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKTKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35263..57569
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FC640_RS23890 (FC640_23890) | 30588..31241 | - | 654 | WP_170965938 | hypothetical protein | - |
| FC640_RS23895 (FC640_23895) | 31253..32377 | - | 1125 | WP_000486716 | site-specific integrase | - |
| FC640_RS24205 | 32790..33011 | - | 222 | Protein_35 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| FC640_RS23900 (FC640_23900) | 33056..33316 | + | 261 | WP_029237379 | hypothetical protein | - |
| FC640_RS23905 (FC640_23905) | 33295..34611 | - | 1317 | WP_074526577 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| FC640_RS23910 (FC640_23910) | 34624..35259 | - | 636 | WP_000934978 | A24 family peptidase | - |
| FC640_RS23915 (FC640_23915) | 35263..35745 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| FC640_RS23920 (FC640_23920) | 35812..36375 | - | 564 | WP_097479680 | type 4 pilus major pilin | - |
| FC640_RS23925 (FC640_23925) | 36425..37534 | - | 1110 | WP_153674374 | type II secretion system F family protein | - |
| FC640_RS23930 (FC640_23930) | 37525..39063 | - | 1539 | WP_000466225 | GspE/PulE family protein | virB11 |
| FC640_RS23935 (FC640_23935) | 39088..39582 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| FC640_RS23940 (FC640_23940) | 39566..40876 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| FC640_RS23945 (FC640_23945) | 40927..42570 | - | 1644 | WP_054173908 | PilN family type IVB pilus formation outer membrane protein | - |
| FC640_RS23950 (FC640_23950) | 42621..44579 | - | 1959 | WP_254521165 | type IV secretory system conjugative DNA transfer family protein | - |
| FC640_RS23955 (FC640_23955) | 44595..45650 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| FC640_RS23960 (FC640_23960) | 45669..46808 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| FC640_RS23965 (FC640_23965) | 46798..47499 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| FC640_RS23970 (FC640_23970) | 47565..48299 | - | 735 | WP_000432282 | virB8 family protein | virB8 |
| FC640_RS23975 (FC640_23975) | 48299..48433 | - | 135 | WP_000701233 | hypothetical protein | - |
| FC640_RS23980 (FC640_23980) | 48465..50822 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| FC640_RS23985 (FC640_23985) | 50828..51148 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| FC640_RS23990 (FC640_23990) | 51219..51509 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| FC640_RS23995 (FC640_23995) | 51509..52093 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| FC640_RS24000 (FC640_24000) | 52114..52512 | - | 399 | WP_001153669 | hypothetical protein | - |
| FC640_RS24005 (FC640_24005) | 52631..53068 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| FC640_RS24010 (FC640_24010) | 53074..54309 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| FC640_RS24015 (FC640_24015) | 54312..54611 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| FC640_RS24020 (FC640_24020) | 54659..55468 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| FC640_RS24025 (FC640_24025) | 55691..55915 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| FC640_RS24030 (FC640_24030) | 55924..56568 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| FC640_RS24035 (FC640_24035) | 56574..57569 | - | 996 | WP_065304446 | type IV secretion system protein | virB6 |
| FC640_RS24040 (FC640_24040) | 57573..57830 | - | 258 | WP_000739144 | hypothetical protein | - |
| FC640_RS24045 (FC640_24045) | 57827..58129 | - | 303 | WP_065304447 | hypothetical protein | - |
| FC640_RS24050 (FC640_24050) | 58145..58360 | - | 216 | WP_001360344 | hypothetical protein | - |
| FC640_RS24055 (FC640_24055) | 58344..58652 | - | 309 | WP_236492988 | hypothetical protein | - |
| FC640_RS24060 (FC640_24060) | 58699..58869 | - | 171 | WP_044069355 | hypothetical protein | - |
| FC640_RS24065 (FC640_24065) | 58873..59316 | - | 444 | WP_000498521 | NfeD family protein | - |
| FC640_RS24070 (FC640_24070) | 59378..60331 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| FC640_RS24075 (FC640_24075) | 60377..60571 | - | 195 | WP_001127356 | DUF1187 family protein | - |
| FC640_RS24080 (FC640_24080) | 60564..61016 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 14534 | GenBank | NZ_CP100687 |
| Plasmid name | pR18.1297_62k | Incompatibility group | IncI2 |
| Plasmid size | 62147 bp | Coordinate of oriT [Strand] | 12803..12855 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Goldcoast strain R18.1297 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |