Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114046
Name   oriT_pYULZMPS10 in_silico
Organism   Salmonella enterica subsp. enterica serovar London strain LZ19MPS10
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP100354 (67797..67947 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      61..67, 73..79  (ATGCTCA..TGAGCAT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pYULZMPS10
TGGTCGATGTGAACCCTTTTGACCGGGTTATGAATGAATTGAAAAATCGTGGCCGCAAAAATGCTCATATCCTGAGCATCCTTCAATTCGATTGGCCTGCATCAGAGGTCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGTATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9025 GenBank   WP_176453579
Name   mobH_NLL85_RS00430_pYULZMPS10 insolico UniProt ID   _
Length   989 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 989 a.a.        Molecular weight: 109277.46 Da        Isoelectric Point: 4.7510

>WP_176453579.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MLKALNKLFGGRSGVIEATPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHGIERYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTTVNQPVTRLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEATGSLKILMLRLESNDLVFTNEPPSAVAAEVVGDVEDAEIE
FVDPDESDDDQDEGGVSLNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSTEPEDTPAQGAAETKDSPKAT
SATTPSKGKQVKAKPKKDTEEQTEKPEAKEELSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDAF
NASTDACIADEESLKNSNEAELEQPKPDFVPEEQNSLQDDDFPMFSGSDEPPSWAIEPLPMLAVESEEAS
ATTEMSQANIPNQQEKDAKTLLSEMLAGYGEASALLEQAIMPVLEGKKTLGEVLCLMKGQAVILYPEGAR
SLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAQQLSDAVVAAIKEVEASMGGYQDVFELVS
PPDSDVRKSKSAPKQQSQPMQKKNQQQKSDAGSGQAAPAKDTKGKAAQKPQKEKKGDTSSVVAGHESKPI
EEKQNVARLPKREAQPEAVVVTKVEREKELGHIEVREREEQEVREFEPPKAKTNPKDIKDEDFLPPGVTP
EKAILILKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGINKHILCGMLSRAQKRPLLKKRQG
KLYLEVDET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   10455 GenBank   WP_022635536
Name   traD_NLL85_RS00435_pYULZMPS10 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69430.92 Da        Isoelectric Point: 7.1173

>WP_022635536.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLRGRDLEFISITELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKRKELPIGQPWIHGVEPKEEKLMQPLKHTEGHTLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIRAVQAYSERVRPDWEAEAAPFLEKVKNGSREKIAFA
LMRFYYEIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSTDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-292)

  Protein structure



No available structure.



ID   10456 GenBank   WP_254155507
Name   traC_NLL85_RS00520_pYULZMPS10 insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 92236.42 Da        Isoelectric Point: 5.9175

>WP_254155507.1 type IV secretion system protein TraC [Salmonella enterica]
MIVTIKKKLQETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKATLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRISAKTNKGTYDNGLIQ
DLKLFVTCKVPINNNNPSESELQNLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
AVDWEMDKPICEQIFDYGTDVEVSKNGLRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKISYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWD
EKGKEMKVDDIAECCLEGEDMRLRDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRNRKQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-808)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 65881..90978

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLL85_RS00420 61915..62148 - 234 WP_176453578 hypothetical protein -
NLL85_RS00425 62130..62747 - 618 WP_071681756 hypothetical protein -
NLL85_RS00430 62915..65884 + 2970 WP_176453579 MobH family relaxase -
NLL85_RS00435 65881..67746 + 1866 WP_022635536 conjugative transfer system coupling protein TraD virb4
NLL85_RS00440 67757..68341 + 585 WP_031280871 hypothetical protein -
NLL85_RS00445 68298..68927 + 630 WP_022635537 DUF4400 domain-containing protein tfc7
NLL85_RS00450 68936..69382 + 447 WP_071681752 hypothetical protein -
NLL85_RS00455 69392..69769 + 378 WP_013141541 hypothetical protein -
NLL85_RS00460 69769..70431 + 663 WP_254155563 hypothetical protein -
NLL85_RS00465 70612..70755 + 144 WP_001275801 hypothetical protein -
NLL85_RS00470 70767..71129 + 363 WP_231897578 hypothetical protein -
NLL85_RS00475 71141..71308 + 168 WP_176453581 hypothetical protein -
NLL85_RS00480 71277..71558 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
NLL85_RS00485 71555..72181 + 627 WP_022635539 TraE/TraK family type IV conjugative transfer system protein traE
NLL85_RS00490 72165..73082 + 918 WP_022635540 type-F conjugative transfer system secretin TraK traK
NLL85_RS00495 73082..74401 + 1320 WP_071681777 TraB/VirB10 family protein traB
NLL85_RS00500 74398..74976 + 579 WP_010845916 type IV conjugative transfer system lipoprotein TraV traV
NLL85_RS00505 74989..75372 + 384 WP_089140452 TraA family conjugative transfer protein -
NLL85_RS00510 75576..81062 + 5487 WP_254155564 Ig-like domain-containing protein -
NLL85_RS00515 81211..81918 + 708 WP_089140450 DsbC family protein trbB
NLL85_RS00520 81915..84359 + 2445 WP_254155507 type IV secretion system protein TraC virb4
NLL85_RS00525 84374..84691 + 318 WP_013141553 hypothetical protein -
NLL85_RS00530 84688..85218 + 531 WP_022635546 S26 family signal peptidase -
NLL85_RS00535 85181..86446 + 1266 WP_089140448 TrbC family F-type conjugative pilus assembly protein traW
NLL85_RS00540 86443..87114 + 672 WP_071681744 EAL domain-containing protein -
NLL85_RS00545 87135..88118 + 984 WP_240433690 TraU family protein traU
NLL85_RS00550 88234..90978 + 2745 WP_119828715 conjugal transfer mating pair stabilization protein TraN traN
NLL85_RS00555 91016..91879 - 864 WP_254155509 hypothetical protein -
NLL85_RS00560 92001..92642 - 642 WP_041979271 hypothetical protein -
NLL85_RS00565 92936..93256 + 321 WP_013141561 hypothetical protein -
NLL85_RS00570 93561..93746 + 186 WP_231858731 hypothetical protein -
NLL85_RS00575 93966..94934 + 969 WP_113620223 AAA family ATPase -
NLL85_RS00580 94945..95850 + 906 WP_254155510 hypothetical protein -

Host bacterium

ID   14481 GenBank   NZ_CP100354
Plasmid name   pYULZMPS10 Incompatibility group   IncA/C2
Plasmid size   158615 bp Coordinate of oriT [Strand]   67797..67947 [+]
Host baterium   Salmonella enterica subsp. enterica serovar London strain LZ19MPS10

Cargo genes

Drug resistance gene   mcr-4.3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -