Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114040
Name   oriT_pDRC3C in_silico
Organism   Lactococcus lactis subsp. lactis strain DRC3
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP064838 (40057..40193 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pDRC3C
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9021 GenBank   WP_095348645
Name   Relaxase_LLDRC3_RS13485_pDRC3C insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47716.32 Da        Isoelectric Point: 8.1252

>WP_095348645.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   14475 GenBank   NZ_CP064838
Plasmid name   pDRC3C Incompatibility group   -
Plasmid size   43493 bp Coordinate of oriT [Strand]   40057..40193 [+]
Host baterium   Lactococcus lactis subsp. lactis strain DRC3

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -