Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114016
• Name: oriT_pEpi0143-OL-4
• Organism: Macrococcus equipercicus strain Epi0143-OL
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP073813 (1358..1455 [+], 98 nt)
• oriT length: 98 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 98 nt

>oriT_pEpi0143-OL-4
TCGGCTGAAACTTTGAATATGAGTGTGGCTGCTTATGCGAAGAAGAAGGCACAGAATAGTCGTGTGGTCGCACCAAAATTGGATGCGGAGACACGACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_KFV11_RS11585_pEpi0143-OL-4

• ID: 9005
• GenBank: WP_254250644
• Name: Rep_trans_KFV11_RS11585_pEpi0143-OL-4
• UniProt ID: _
• Length: 296 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 296 a.a.
• Molecular weight: 35274.33 Da
• Isoelectric Point: 6.1775

>WP_254250644.1 replication initiation factor domain-containing protein [Macrococcus equipercicus]
MGKDELEKNWEPALSNSRLSGSFFTTPQPKISFDRMTIVGDLPKNRAEYMADLLGNSTFVQLWDCLTHKF
KGQIFSDKVYVEHDRFKADAWDRRNFRIEFNPNNLTADEMSWLKKNILVALENVGFTRLDLAFDFEKDLS
DFFIMSDKALKKTVFYGCDGKAETKYFGVRDSDRFVRIYNKKQERKDNADIEMELEDFWRFEIEIKRTMV
DGWKNCFDDMHILKPDWKTVEKISDRGILFLLLNDESEWGKLERHQKYKFKKLVKEISPVDLTDLMKLSL
REQENKLQKQIDFWLS

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (126-209)

Protein structure

No available structure.

Host bacterium

• ID: 14451
• GenBank: NZ_CP073813
• Plasmid name: pEpi0143-OL-4
• Incompatibility group: -
• Plasmid size: 4333 bp
• Coordinate of oriT [Strand]: 1358..1455 [+]
• Host baterium: Macrococcus equipercicus strain Epi0143-OL

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -