Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114005
Name   oriT_pPV835TEM24 in_silico
Organism   Proteus vulgaris strain PV835
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MN148427 (58553..58703 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pPV835TEM24
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8998 GenBank   WP_001337757
Name   mobH_HTY06_RS00410_pPV835TEM24 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   10432 GenBank   WP_000178856
Name   traD_HTY06_RS00415_pPV835TEM24 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   10433 GenBank   WP_000637386
Name   traC_HTY06_RS00495_pPV835TEM24 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56637..81828

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTY06_RS00400 52662..52895 - 234 WP_001191892 hypothetical protein -
HTY06_RS00405 52877..53494 - 618 WP_001249396 hypothetical protein -
HTY06_RS00410 53662..56640 + 2979 WP_001337757 MobH family relaxase -
HTY06_RS00415 56637..58502 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
HTY06_RS00420 58513..59097 + 585 WP_000332866 hypothetical protein -
HTY06_RS00425 59054..59683 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
HTY06_RS00430 59693..60139 + 447 WP_000122506 hypothetical protein -
HTY06_RS00435 60149..60526 + 378 WP_000869261 hypothetical protein -
HTY06_RS00440 60526..61188 + 663 WP_001231463 hypothetical protein -
HTY06_RS00445 61369..61512 + 144 WP_001275801 hypothetical protein -
HTY06_RS00450 61524..61889 + 366 WP_001052531 hypothetical protein -
HTY06_RS00455 62034..62315 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HTY06_RS00460 62312..62938 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
HTY06_RS00465 62922..63839 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HTY06_RS00470 63839..65152 + 1314 WP_024131605 TraB/VirB10 family protein traB
HTY06_RS00475 65149..65727 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HTY06_RS00480 65731..66123 + 393 WP_000479535 TraA family conjugative transfer protein -
HTY06_RS00485 66324..71855 + 5532 WP_000606833 Ig-like domain-containing protein -
HTY06_RS00490 72004..72711 + 708 WP_001259347 DsbC family protein -
HTY06_RS00495 72708..75155 + 2448 WP_000637386 type IV secretion system protein TraC virb4
HTY06_RS00500 75170..75487 + 318 WP_000351984 hypothetical protein -
HTY06_RS00505 75484..76014 + 531 WP_001010738 S26 family signal peptidase -
HTY06_RS00510 75977..77242 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
HTY06_RS00515 77239..77910 + 672 WP_001337754 EAL domain-containing protein -
HTY06_RS00520 77910..78914 + 1005 WP_043940352 TraU family protein traU
HTY06_RS00525 79030..81828 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
HTY06_RS00530 81867..82727 - 861 WP_000709517 hypothetical protein -
HTY06_RS00535 82850..83491 - 642 WP_000796664 hypothetical protein -
HTY06_RS00540 83785..84105 + 321 WP_000547566 hypothetical protein -
HTY06_RS00545 84409..84594 + 186 WP_001186917 hypothetical protein -
HTY06_RS00550 84814..85782 + 969 WP_000085162 AAA family ATPase -
HTY06_RS00555 85793..86701 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   14440 GenBank   NZ_MN148427
Plasmid name   pPV835TEM24 Incompatibility group   IncA/C2
Plasmid size   174086 bp Coordinate of oriT [Strand]   58553..58703 [+]
Host baterium   Proteus vulgaris strain PV835

Cargo genes

Drug resistance gene   floR, sul2, sul1, qacE, blaTEM-24, dfrA1, ant(3'')-Ia
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -