Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114003
Name   oriT_R805a in_silico
Organism   Salmonella enterica subsp. enterica strain H-185
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK088173 (29356..29440 [-], 85 nt)
oriT length   85 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 85 nt

>oriT_R805a
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCATTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8996 GenBank   WP_083574966
Name   Relaxase_HTW33_RS00200_R805a insolico UniProt ID   _
Length   906 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 906 a.a.        Molecular weight: 104942.63 Da        Isoelectric Point: 8.2349

>WP_083574966.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIDQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDKAREGIALSSFGPSWAAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure



No available structure.




Auxiliary protein

ID   5245 GenBank   WP_001291057
Name   WP_001291057_R805a insolico UniProt ID   A0A142CND4
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12596.69 Da        Isoelectric Point: 10.7335

>WP_001291057.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVSTLIRKTVLGKQINARIDEDFLKELMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITELANTLRRNLMGR

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF6290) (DUF6290)

(19-66)


  Protein structure


Source ID Structure
AlphaFold DB A0A142CND4


T4CP

ID   10430 GenBank   WP_033816586
Name   trbC_HTW33_RS00205_R805a insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 86890.73 Da        Isoelectric Point: 6.5117

>WP_033816586.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKVILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 35021..73866

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTW33_RS00205 32740..35040 - 2301 WP_033816586 F-type conjugative transfer protein TrbC -
HTW33_RS00210 35021..36145 - 1125 WP_083574964 DsbC family protein trbB
HTW33_RS00215 36142..37449 - 1308 WP_021577249 IncI1-type conjugal transfer protein TrbA trbA
HTW33_RS00485 38246..38341 + 96 WP_001363141 DinQ-like type I toxin DqlB -
HTW33_RS00225 38404..38580 - 177 WP_032172741 hypothetical protein -
HTW33_RS00230 38730..39197 - 468 WP_000598962 thermonuclease family protein -
HTW33_RS00235 39356..39979 + 624 WP_001155252 helix-turn-helix domain-containing protein -
HTW33_RS00240 40177..40392 - 216 WP_000266544 hypothetical protein -
HTW33_RS00245 40396..40764 - 369 WP_143410569 hypothetical protein -
HTW33_RS00250 41064..41306 - 243 WP_001401586 hypothetical protein -
HTW33_RS00255 41636..41788 + 153 WP_001302699 Hok/Gef family protein -
HTW33_RS00260 41926..43512 - 1587 WP_000004560 TnsA endonuclease N-terminal domain-containing protein -
HTW33_RS00265 43786..44436 - 651 WP_001044247 plasmid IncI1-type surface exclusion protein ExcA -
HTW33_RS00270 44525..46690 - 2166 WP_000691810 DotA/TraY family protein traY
HTW33_RS00275 46765..47334 - 570 WP_000650752 conjugal transfer protein TraX -
HTW33_RS00280 47331..48536 - 1206 WP_001189549 conjugal transfer protein TraW traW
HTW33_RS00285 48494..49114 - 621 WP_000286854 hypothetical protein traV
HTW33_RS00290 49114..52158 - 3045 WP_001024766 conjugal transfer protein traU
HTW33_RS00295 52209..52460 + 252 WP_001136183 hypothetical protein -
HTW33_RS00300 52453..53079 - 627 WP_000785703 hypothetical protein traT
HTW33_RS00490 53186..53437 - 252 WP_001277573 hypothetical protein -
HTW33_RS00310 53494..53892 - 399 WP_000088813 DUF6750 family protein traR
HTW33_RS00315 53939..54469 - 531 WP_000987021 conjugal transfer protein TraQ traQ
HTW33_RS00320 54466..55179 - 714 WP_000787003 conjugal transfer protein TraP traP
HTW33_RS00325 55176..56513 - 1338 WP_001272018 conjugal transfer protein TraO traO
HTW33_RS00330 56517..57491 - 975 WP_000547385 DotH/IcmK family type IV secretion protein traN
HTW33_RS00335 57502..58197 - 696 WP_001287545 DotI/IcmL family type IV secretion protein traM
HTW33_RS00340 58209..58559 - 351 WP_001056367 hypothetical protein traL
HTW33_RS00345 58576..62637 - 4062 WP_000842453 LPD7 domain-containing protein -
HTW33_RS00350 62701..62991 - 291 WP_000817801 IcmT/TraK family protein traK
HTW33_RS00355 62988..64136 - 1149 WP_000775210 plasmid transfer ATPase TraJ virB11
HTW33_RS00360 64120..64956 - 837 WP_000745049 type IV secretory system conjugative DNA transfer family protein traI
HTW33_RS00365 64953..65411 - 459 WP_001170111 DotD/TraH family lipoprotein -
HTW33_RS00370 65515..66717 - 1203 WP_000979994 conjugal transfer protein TraF -
HTW33_RS00375 66819..67640 - 822 WP_000845373 hypothetical protein traE
HTW33_RS00480 67805..67999 - 195 Protein_74 integrase -
HTW33_RS00380 68113..69474 - 1362 WP_001168171 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTW33_RS00385 69492..70028 - 537 WP_023649457 prepilin peptidase -
HTW33_RS00390 70134..70619 - 486 WP_001080767 lytic transglycosylase domain-containing protein virB1
HTW33_RS00395 70664..71200 - 537 WP_000111534 type 4 pilus major pilin -
HTW33_RS00400 71262..72356 - 1095 WP_000697158 type II secretion system F family protein -
HTW33_RS00405 72358..73866 - 1509 WP_000880805 GspE/PulE family protein virB11
HTW33_RS00410 73970..74428 - 459 WP_000095885 type IV pilus biogenesis protein PilP -
HTW33_RS00415 74418..75713 - 1296 WP_000129883 type 4b pilus protein PilO2 -
HTW33_RS00420 75734..77353 - 1620 WP_001035565 PilN family type IVB pilus formation outer membrane protein -
HTW33_RS00425 77385..77798 - 414 WP_124983997 type IV pilus biogenesis protein PilM -

Host bacterium

ID   14438 GenBank   NZ_MK088173
Plasmid name   R805a Incompatibility group   IncB/O/K/Z
Plasmid size   84157 bp Coordinate of oriT [Strand]   29356..29440 [-]
Host baterium   Salmonella enterica subsp. enterica strain H-185

Cargo genes

Drug resistance gene   aph(3')-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -