Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113999 |
| Name | oriT_p707804-3FII  |
| Organism | Leclercia adecarboxylata strain 150707804 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MH909329 (63780..63835 [-], 56 nt) |
| oriT length | 56 nt |
| IRs (inverted repeats) | 22..28, 32..38 (GCAAGAC..GTCTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 56 nt
>oriT_p707804-3FII
ATGAATTTAAAGATAAAATCTGCAAGACAACGTCTTGCGTGGGGTGTGATGTTTTT
ATGAATTTAAAGATAAAATCTGCAAGACAACGTCTTGCGTGGGGTGTGATGTTTTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8993 | GenBank | WP_172693799 |
| Name | traI_HTU55_RS00500_p707804-3FII |
UniProt ID | _ |
| Length | 1810 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1810 a.a. Molecular weight: 197546.84 Da Isoelectric Point: 6.5095
>WP_172693799.1 conjugative transfer relaxase/helicase TraI [Leclercia adecarboxylata]
MMSVAPVASAGSAAGYYSNSDNYYFLGNLQSLWLGEGAKELGLEGNVRGEDLTAVLEGRLPDGSRLGREI
NGNHVHRPGHDLTFSAPKSVSILALIGNDKAMLEAHNHAVRVAAGYVEKLISARVTKDGVTSIAHTGKMV
AAAFTHDTSRNLDPLIHTHLLIANMTEHEGKWKALATDYIHNAGFIETVMKHQVTLGKIYRGALRQRVET
LGHEVEEVGKHGMWEIKAIPEEVREEYSSRGREIRGAVGADATLRSRDVAAKDTRQAKVDPSRLRLMERW
LGQMKEKGFDLKAYQESVVPRESGLRDVPLPTERVSPVPADRTEPGAVARSAPEDKTGKNPELPALPAER
DDTARQPGSHLADTLRQPSLTEPEKTPERSVSLPARDLHADTPQPDLAEAVRLAISQLSDARTRFTFGEL
MLTTAELSEQLPEIRDIRVAIDAALKDGMIVPLDSEKGVFTSRIHLLDELSIQALSQEHLKSTKVVSFTR
PAQYAPPALAVAETDALVLMNAPSGVAGIRDLTVQLTGISTANGRDVQVLASSAERAISLAKSDTLRDRL
VSRQHILSGDFHLKPQSTLIIEGAERLGLKETLVLLGEARAQDAQLVFLDSAGRQANGNAMSVLESAGVA
RSRRTEPAPGLEAEAISIANKRDRYEALANRFAELSGGSENVTAVVVGKREQAQLTGLVRDALQNAGQLG
RDGAEIEARQPVWLDSKTRRMPGSYRPGMVLEDRRDAKARKSYVIDRVHDDTRVLSLVDGDGVLTRMKIG
EVSADWRLFSSETLHVATGEKLLAVAGDREHSLKAKDRLEVTAITGKGIEVKRGDETLTLPKDQPLYVTH
AYVSAPGGRDNDTGVVLAALNTRDISTQTMNSLAQSGHRAEVFTAEVQDRAEARLQRMKTNASPVQLVRT
LSGKDDVSEAVSTLRDNVQSDAELAVWRAVNDQRHVTFSELKLADEAVKFHPDFDAIDGQIRAMVKKGDL
LEVSVGGEPKLVARSTWEMEKAILRVVEEGKNTQQPLLDQVPADVLSGLTRGQKAATSLVLGSTDQFTGV
QGYAGVGKTTQMKAVMAALDTLPTDARPVLTGLAPTHQAVKEMSDVGVQARTIKSFIVEHDQATAAGEKP
DYKGQMFLIDESSMAGNQDTAALFQAIAAGGGRAVSMGDVDQFEAVDSGAPFRLMQERSPMDVAIMKEIV
RQKDVQLRGAVHDIIDNRIGAALRRIEAQPADRISREAGAKAPGSAFQETGSPVQDIVRDWTERTADARD
RTLIITQLNADRRAVNVGIHRQLAERGELGDKAITIPVLDKISHTRHEFNQMKPWASGMVVKRGDRYQDV
LAVDRNGNTITVRDEEGKIGLYSPKELITGDVQLFKRKTIEVRSGDQLRFTATDREQGMTANQRFTVDAV
SDTGDIRLRGTDGVVTINPKDIRAQQHVDYAWAVTGYGAQGTSTDYVISLEGTSGGREMMATRRAFYITA
SRAKEHVQIYTDGKKDWTKAVTKPEGDIKTAHEALAPETQRQQAKAIWAMGQPLTKTAIGRAWVRHQDMG
EASLTAKVIPATRRFPEPALALPVYDNNGKSAGLALVSLVSSPEGRLTQGDTRMVMTERARGAVLQRSQS
GNTVVVNDLAAALDAVRTHPKDGVVWQTGQEPLSAHLLKVTGGVRGDEEIRLNFGNLPGQDELQRQSELK
LAEQAREQEAVRQQPDDARAIVLPAEESVKLRPEELNPEKTVPLTPDSWVLASVREREQPDARELQLADS
VRKDIAVPDKSAGEESRASRIIADLARAEQDRVRQTSDSGRGRMPEIEEQTLTRTIQKER
MMSVAPVASAGSAAGYYSNSDNYYFLGNLQSLWLGEGAKELGLEGNVRGEDLTAVLEGRLPDGSRLGREI
NGNHVHRPGHDLTFSAPKSVSILALIGNDKAMLEAHNHAVRVAAGYVEKLISARVTKDGVTSIAHTGKMV
AAAFTHDTSRNLDPLIHTHLLIANMTEHEGKWKALATDYIHNAGFIETVMKHQVTLGKIYRGALRQRVET
LGHEVEEVGKHGMWEIKAIPEEVREEYSSRGREIRGAVGADATLRSRDVAAKDTRQAKVDPSRLRLMERW
LGQMKEKGFDLKAYQESVVPRESGLRDVPLPTERVSPVPADRTEPGAVARSAPEDKTGKNPELPALPAER
DDTARQPGSHLADTLRQPSLTEPEKTPERSVSLPARDLHADTPQPDLAEAVRLAISQLSDARTRFTFGEL
MLTTAELSEQLPEIRDIRVAIDAALKDGMIVPLDSEKGVFTSRIHLLDELSIQALSQEHLKSTKVVSFTR
PAQYAPPALAVAETDALVLMNAPSGVAGIRDLTVQLTGISTANGRDVQVLASSAERAISLAKSDTLRDRL
VSRQHILSGDFHLKPQSTLIIEGAERLGLKETLVLLGEARAQDAQLVFLDSAGRQANGNAMSVLESAGVA
RSRRTEPAPGLEAEAISIANKRDRYEALANRFAELSGGSENVTAVVVGKREQAQLTGLVRDALQNAGQLG
RDGAEIEARQPVWLDSKTRRMPGSYRPGMVLEDRRDAKARKSYVIDRVHDDTRVLSLVDGDGVLTRMKIG
EVSADWRLFSSETLHVATGEKLLAVAGDREHSLKAKDRLEVTAITGKGIEVKRGDETLTLPKDQPLYVTH
AYVSAPGGRDNDTGVVLAALNTRDISTQTMNSLAQSGHRAEVFTAEVQDRAEARLQRMKTNASPVQLVRT
LSGKDDVSEAVSTLRDNVQSDAELAVWRAVNDQRHVTFSELKLADEAVKFHPDFDAIDGQIRAMVKKGDL
LEVSVGGEPKLVARSTWEMEKAILRVVEEGKNTQQPLLDQVPADVLSGLTRGQKAATSLVLGSTDQFTGV
QGYAGVGKTTQMKAVMAALDTLPTDARPVLTGLAPTHQAVKEMSDVGVQARTIKSFIVEHDQATAAGEKP
DYKGQMFLIDESSMAGNQDTAALFQAIAAGGGRAVSMGDVDQFEAVDSGAPFRLMQERSPMDVAIMKEIV
RQKDVQLRGAVHDIIDNRIGAALRRIEAQPADRISREAGAKAPGSAFQETGSPVQDIVRDWTERTADARD
RTLIITQLNADRRAVNVGIHRQLAERGELGDKAITIPVLDKISHTRHEFNQMKPWASGMVVKRGDRYQDV
LAVDRNGNTITVRDEEGKIGLYSPKELITGDVQLFKRKTIEVRSGDQLRFTATDREQGMTANQRFTVDAV
SDTGDIRLRGTDGVVTINPKDIRAQQHVDYAWAVTGYGAQGTSTDYVISLEGTSGGREMMATRRAFYITA
SRAKEHVQIYTDGKKDWTKAVTKPEGDIKTAHEALAPETQRQQAKAIWAMGQPLTKTAIGRAWVRHQDMG
EASLTAKVIPATRRFPEPALALPVYDNNGKSAGLALVSLVSSPEGRLTQGDTRMVMTERARGAVLQRSQS
GNTVVVNDLAAALDAVRTHPKDGVVWQTGQEPLSAHLLKVTGGVRGDEEIRLNFGNLPGQDELQRQSELK
LAEQAREQEAVRQQPDDARAIVLPAEESVKLRPEELNPEKTVPLTPDSWVLASVREREQPDARELQLADS
VRKDIAVPDKSAGEESRASRIIADLARAEQDRVRQTSDSGRGRMPEIEEQTLTRTIQKER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 10428 | GenBank | WP_172693820 |
| Name | traD_HTU55_RS00495_p707804-3FII |
UniProt ID | _ |
| Length | 871 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 871 a.a. Molecular weight: 97068.09 Da Isoelectric Point: 5.2053
>WP_172693820.1 type IV conjugative transfer system coupling protein TraD [Leclercia adecarboxylata]
MSFAGKNLTQGGQMTAYRWRMFLQVNNWIGFWMFIFFAVLTSAIFLWHIPGETLNNGSLWWFAWMNSSFI
DLMPTTGTPKIYDVHYWYAPTATAMVLNMTLPQIYTDPYMQAMGDQVLSELQWAAGASGIFSMVVFAAVT
WFIADIGRKESEDEYISGMQLTDRPAEVNSLLRKSGELSDLRVGDLHMVRMAEVLNFLMHGTIGVGKSTL
IRWLLDHIRKRGDRAIIYDSGCTFTETHYNPSTDFILNAHDERCANWQMWGECIDAVDYDNLAASLIPVE
GESDPFWVSSSRTIFADLAIRMSADPQRSIEKFLKTLLSLSMKSLRDYLANTPSANLVEEKIEKTAISIR
SVVTNYAKALRYLQGLDDGTKPPFTIREWMTQERYDSSWLFISTQARHRKSVRPLISLWVSLATLMLQSM
GENSERRVWFIIDEAPSLQRIPELAETLAEARKFGGCFVLGMQNMAQLVHVYGRELAKSIFDLMNTRMYG
RSPSAEMAKVVEEELGNQRRRKIREQNSYGLDQVRDGVSLGKDEVNEPVVDYEQIMRLPNLSFYVRLPGE
YPVVRLKLKYRALKKRHAGLMERNIRDALSPELEKVIQENERAATAAGLTFPTGDEVLEKGTETTPAAGS
TAPVSSAGAPATLSPCEEKPVTEPATGKKPARVMPVIRNLQPVAVPVMSGAHATIAEAAPDNHNTVTAPV
TSSEKSNVISLHQGRHSPADSSPEASMTDESAPSTPSEPDIAETPAEQVALPAALQRLRNMTTAAEQPAT
SVLSILQKARQRPATSENNTGTGDNNGGESMNLEMKVTALPDGGLSVTTAGKPVTPANPEEAHRTNRQLA
QEEENILLHRDAGDPGYDENDQYYYDREPEL
MSFAGKNLTQGGQMTAYRWRMFLQVNNWIGFWMFIFFAVLTSAIFLWHIPGETLNNGSLWWFAWMNSSFI
DLMPTTGTPKIYDVHYWYAPTATAMVLNMTLPQIYTDPYMQAMGDQVLSELQWAAGASGIFSMVVFAAVT
WFIADIGRKESEDEYISGMQLTDRPAEVNSLLRKSGELSDLRVGDLHMVRMAEVLNFLMHGTIGVGKSTL
IRWLLDHIRKRGDRAIIYDSGCTFTETHYNPSTDFILNAHDERCANWQMWGECIDAVDYDNLAASLIPVE
GESDPFWVSSSRTIFADLAIRMSADPQRSIEKFLKTLLSLSMKSLRDYLANTPSANLVEEKIEKTAISIR
SVVTNYAKALRYLQGLDDGTKPPFTIREWMTQERYDSSWLFISTQARHRKSVRPLISLWVSLATLMLQSM
GENSERRVWFIIDEAPSLQRIPELAETLAEARKFGGCFVLGMQNMAQLVHVYGRELAKSIFDLMNTRMYG
RSPSAEMAKVVEEELGNQRRRKIREQNSYGLDQVRDGVSLGKDEVNEPVVDYEQIMRLPNLSFYVRLPGE
YPVVRLKLKYRALKKRHAGLMERNIRDALSPELEKVIQENERAATAAGLTFPTGDEVLEKGTETTPAAGS
TAPVSSAGAPATLSPCEEKPVTEPATGKKPARVMPVIRNLQPVAVPVMSGAHATIAEAAPDNHNTVTAPV
TSSEKSNVISLHQGRHSPADSSPEASMTDESAPSTPSEPDIAETPAEQVALPAALQRLRNMTTAAEQPAT
SVLSILQKARQRPATSENNTGTGDNNGGESMNLEMKVTALPDGGLSVTTAGKPVTPANPEEAHRTNRQLA
QEEENILLHRDAGDPGYDENDQYYYDREPEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65821..91962
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTU55_RS00330 | 61897..62190 | + | 294 | WP_172693768 | hypothetical protein | - |
| HTU55_RS00335 | 62202..63023 | + | 822 | WP_172693769 | DUF932 domain-containing protein | - |
| HTU55_RS00340 | 63051..63590 | - | 540 | WP_172693819 | lytic transglycosylase domain-containing protein | - |
| HTU55_RS00345 | 64201..64587 | + | 387 | WP_172693770 | relaxosome protein TraM | - |
| HTU55_RS00350 | 64626..65267 | - | 642 | WP_172693771 | Arc family DNA-binding protein | - |
| HTU55_RS00355 | 65464..65817 | + | 354 | WP_172693772 | type IV conjugative transfer system pilin TraA | - |
| HTU55_RS00360 | 65821..66123 | + | 303 | WP_172693773 | type IV conjugative transfer system protein TraL | traL |
| HTU55_RS00365 | 66129..66713 | + | 585 | WP_172693774 | TraE/TraK family type IV conjugative transfer system protein | traE |
| HTU55_RS00370 | 66725..67465 | + | 741 | WP_172693775 | type-F conjugative transfer system secretin TraK | traK |
| HTU55_RS00375 | 67462..68865 | + | 1404 | WP_172693776 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HTU55_RS00380 | 68876..69316 | + | 441 | WP_172693777 | hypothetical protein | - |
| HTU55_RS00385 | 69310..69771 | + | 462 | WP_172693778 | hypothetical protein | - |
| HTU55_RS00390 | 69802..70197 | + | 396 | WP_172693779 | hypothetical protein | - |
| HTU55_RS00395 | 70255..70818 | + | 564 | WP_172693780 | type IV conjugative transfer system lipoprotein TraV | traV |
| HTU55_RS00400 | 70815..73415 | + | 2601 | WP_172693781 | type IV secretion system protein TraC | virb4 |
| HTU55_RS00405 | 73412..73816 | + | 405 | WP_172693782 | type-F conjugative transfer system protein TrbI | - |
| HTU55_RS00410 | 73813..74448 | + | 636 | WP_172693783 | type-F conjugative transfer system protein TraW | traW |
| HTU55_RS00415 | 74445..74741 | + | 297 | WP_172693784 | hypothetical protein | - |
| HTU55_RS00420 | 74732..75256 | + | 525 | WP_172693785 | hypothetical protein | - |
| HTU55_RS00425 | 75328..75537 | + | 210 | WP_172693786 | hypothetical protein | - |
| HTU55_RS00430 | 75669..76613 | + | 945 | WP_230431591 | conjugal transfer pilus assembly protein TraU | traU |
| HTU55_RS00435 | 76623..77240 | + | 618 | WP_172693788 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HTU55_RS00440 | 77237..79096 | + | 1860 | WP_172693789 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HTU55_RS00445 | 79093..79875 | + | 783 | WP_172693790 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HTU55_RS00450 | 79885..80361 | + | 477 | WP_172693791 | hypothetical protein | - |
| HTU55_RS00455 | 80348..80569 | + | 222 | WP_172693792 | hypothetical protein | - |
| HTU55_RS00460 | 80559..81158 | + | 600 | WP_172693793 | F-type conjugal transfer protein TrbB | traF |
| HTU55_RS00465 | 81176..81445 | + | 270 | WP_172693794 | hypothetical protein | - |
| HTU55_RS00470 | 81438..82802 | + | 1365 | WP_172693795 | conjugal transfer pilus assembly protein TraH | traH |
| HTU55_RS00475 | 82802..85939 | + | 3138 | Protein_98 | conjugal transfer mating-pair stabilization protein TraG | - |
| HTU55_RS00480 | 87194..87751 | + | 558 | WP_172693796 | hypothetical protein | - |
| HTU55_RS00485 | 87908..88639 | + | 732 | WP_172693797 | complement resistance protein TraT | - |
| HTU55_RS00495 | 89347..91962 | + | 2616 | WP_172693820 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 14434 | GenBank | NZ_MH909329 |
| Plasmid name | p707804-3FII | Incompatibility group | IncFII |
| Plasmid size | 120182 bp | Coordinate of oriT [Strand] | 63780..63835 [-] |
| Host baterium | Leclercia adecarboxylata strain 150707804 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | pcoE, pcoS, pcoR, pcoD, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |