Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113998
Name   oriT_pEclC2185CTXM15 in_silico
Organism   Enterobacter cloacae strain EclC2185
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK736669 (53064..53318 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pEclC2185CTXM15
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8992 GenBank   WP_012477394
Name   mobH_HTX31_RS00290_pEclC2185CTXM15 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   10427 GenBank   WP_001284073
Name   traD_HTX31_RS00295_pEclC2185CTXM15 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 129491..138698

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTX31_RS00685 124601..125317 - 717 WP_000838219 zinc finger-like domain-containing protein -
HTX31_RS00690 125314..125514 - 201 WP_000594930 hypothetical protein -
HTX31_RS00695 125534..126586 - 1053 WP_001065778 hypothetical protein -
HTX31_RS00700 127063..127938 + 876 WP_000594611 RepB family plasmid replication initiator protein -
HTX31_RS00705 129087..129440 + 354 WP_000423602 hypothetical protein -
HTX31_RS00710 129458..129808 + 351 WP_001447770 type IV conjugative transfer system protein TraL -
HTX31_RS00715 129820..130608 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
HTX31_RS00720 130608..131879 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
HTX31_RS00725 131881..132342 + 462 WP_000521242 plasmid transfer protein HtdO -
HTX31_RS00730 132332..133687 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
HTX31_RS00735 133695..134177 + 483 WP_000377632 hypothetical protein -
HTX31_RS00740 134296..135048 + 753 WP_182911723 protein-disulfide isomerase HtdT -
HTX31_RS00745 135058..136008 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
HTX31_RS00750 136017..138698 + 2682 WP_000387412 TraC family protein virb4
HTX31_RS01655 141247..141408 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   14433 GenBank   NZ_MK736669
Plasmid name   pEclC2185CTXM15 Incompatibility group   IncHI2
Plasmid size   305912 bp Coordinate of oriT [Strand]   53064..53318 [-]
Host baterium   Enterobacter cloacae strain EclC2185

Cargo genes

Drug resistance gene   tet(A), blaOXA-1, aac(6')-Ib-cr, qnrB1, dfrA14, aac(3)-IIa, blaCTX-M-15, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, sul2, ant(3'')-Ia, catA1
Virulence gene   -
Metal resistance gene   pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH, terW, terZ, terA, terB, terC, terD, terE, merE, merD, merB, merR2
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -