Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113994
Name   oriT_pAN70-1 in_silico
Organism   Alcaligenes faecalis strain AN70
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK757441 (12217..12317 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      39..44, 49..54  (CGCACC..GGTGCG)
 13..21, 27..35  (AAGTCATTG..CAATGACTT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  TGTCTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pAN70-1
TCCTTGGCGTGGAAGTCATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTAAGGATACCAACCCGGCTTTTAAGGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8989 GenBank   WP_065164176
Name   mobF_HTW89_RS00105_pAN70-1 insolico UniProt ID   _
Length   962 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 962 a.a.        Molecular weight: 106978.20 Da        Isoelectric Point: 9.8804

>WP_065164176.1 MULTISPECIES: MobF family relaxase [Pseudomonadota]
MVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLAGNIGEGHRIMRSAT
RQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQAEARAQARQKIQGKTRIETTGNLV
IGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRALKNDEIVKATRYLGAVYNAELAHELQKLGYQLRYG
KDGNFDLAHIDRQQIEGFSKRTEQIAEWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQAT
AKELGIDFSRREWSGREKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAA
RLEDIQKELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWVAELAAKGMKQGAARERVDNAIKTGG
LVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREAAELIVSAANRVVGV
QGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEANTLASFLRAKDKNIDSRTVLVIDE
AGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIEAGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVE
LAAAGKASSSLERIKDVTEIKNHHERRAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAG
KGIEFDTLVRVDTTQAERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQ
FSPMTHTKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTDKPLH
VDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGKLPAAIARENIKSAA
HDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAERGMEAGR

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(9-283)

AAA domain (AAA_30)

(469-653)


  Protein structure



No available structure.




Auxiliary protein

ID   5242 GenBank   WP_012196434
Name   WP_012196434_pAN70-1 insolico UniProt ID   A8R762
Length   121 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 121 a.a.        Molecular weight: 13400.32 Da        Isoelectric Point: 4.8464

>WP_012196434.1 MULTISPECIES: hypothetical protein [Pseudomonadota]
MALGDPIQVRLSPEKQALLEDEAARKGKRLATYLRELLESENDLQGELAALRREVVSLHHVIEDLADTGL
RSDQSGPGQNAVQIETLLLLRAIAGPERMKPVKGELKRLGIEVWTPEGKED

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A8R762


T4CP

ID   10425 GenBank   WP_012196433
Name   t4cp2_HTW89_RS00100_pAN70-1 insolico UniProt ID   _
Length   507 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 507 a.a.        Molecular weight: 56272.95 Da        Isoelectric Point: 9.8647

>WP_012196433.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Pseudomonadota]
MHPDDQRKVSAGIVIVLPLIFWITAVQKTEVLGSPKLLALWELMKLTPQKPILLLSALGGLAVGVLFVWL
LNSVGQGEFGGAPFKRFLRGTRIVSGGKLKRMTREKAKQVTVAGVPMPRDAEPRHLLVNGATGTGKSVLL
RELAYTGLLRGDRMVIVDPNGDMLSKFGRDKDIILNPYDQRTKGWSFFNEIRNDYDWQRYALSVVPRGKT
DEAEEWASYGRLLLRETAKKLALIGTPSMRELFHWTTIATFDDLRGFLEGTLAESLFAGSNEASKALTSA
RFVLSDKLPEHVTMPDGDFSIRSWLEDPNGGNLFITWREDMGPALRPLISAWVDVVCTSILSLPEEPKRR
LWLFIDELASLEKLASLADALTKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASFRSLVVLGGSRTDP
KTNEDMSLSLGEHEVERDRYSKNTGKHHSTGRALERVRERVVMPAEIANLPDLTAYVGFAGNRPIAKVPL
EIKQFANRQPAFVEGTI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(110-490)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12779..27416

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTW89_RS00060 (pAN70-1000013) 7859..8182 + 324 WP_012196442 TrfB-related DNA-binding protein -
HTW89_RS00065 (pAN70-1000016) 8875..9219 + 345 WP_012196440 hypothetical protein -
HTW89_RS00070 (pAN70-1000017) 9235..9888 + 654 WP_012196439 hypothetical protein -
HTW89_RS00075 (pAN70-1000018) 10036..10287 + 252 WP_012196438 hypothetical protein -
HTW89_RS00080 (pAN70-1000019) 10441..10827 - 387 WP_012196437 hypothetical protein -
HTW89_RS00085 (pAN70-1000020) 10841..11536 - 696 WP_012196436 StbB family protein -
HTW89_RS00090 (pAN70-1000021) 11533..11958 - 426 WP_012196435 hypothetical protein -
HTW89_RS00095 (pAN70-1000022) 12412..12777 + 366 WP_012196434 hypothetical protein -
HTW89_RS00100 12779..14302 + 1524 WP_012196433 type IV secretion system DNA-binding domain-containing protein virb4
HTW89_RS00105 14314..17202 + 2889 WP_065164176 MobF family relaxase -
HTW89_RS00110 17285..18361 - 1077 WP_012196431 P-type DNA transfer ATPase VirB11 virB11
HTW89_RS00115 18327..19514 - 1188 WP_012196430 type IV secretion system protein VirB10 virB10
HTW89_RS00120 19514..20314 - 801 WP_012196429 P-type conjugative transfer protein VirB9 virB9
HTW89_RS00125 20325..21020 - 696 WP_012196428 virB8 family protein virB8
HTW89_RS00130 21273..22301 - 1029 WP_012196426 type IV secretion system protein virB6
HTW89_RS00135 22315..22545 - 231 WP_012196425 EexN family lipoprotein -
HTW89_RS00140 22542..23231 - 690 WP_012196424 P-type DNA transfer protein VirB5 virB5
HTW89_RS00145 23228..25699 - 2472 WP_012196423 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HTW89_RS00150 25702..26016 - 315 WP_012196422 type IV secretion system protein VirB3 virB3
HTW89_RS00155 26029..26334 - 306 WP_031942907 VirB2 family type IV secretion system major pilin TrwL virB2
HTW89_RS00160 26300..26596 - 297 WP_012196420 KorA family transcriptional regulator -
HTW89_RS00165 26718..27416 - 699 WP_012443549 lytic transglycosylase domain-containing protein virB1
HTW89_RS00170 (pAN70-1000037) 27418..27999 - 582 WP_012196419 hypothetical protein -
HTW89_RS00175 28148..28435 + 288 WP_012414174 H-NS family nucleoid-associated regulatory protein -
HTW89_RS00180 28732..29232 - 501 WP_000376623 GNAT family N-acetyltransferase -
HTW89_RS00185 29360..30211 - 852 WP_000946487 sulfonamide-resistant dihydropteroate synthase Sul1 -
HTW89_RS00190 30313..30771 + 459 Protein_37 class 1 integron integrase IntI1 -
HTW89_RS00200 (pAN70-1000043) 31265..31549 - 285 WP_069953517 hypothetical protein -

Host bacterium

ID   14429 GenBank   NZ_MK757441
Plasmid name   pAN70-1 Incompatibility group   IncW
Plasmid size   61915 bp Coordinate of oriT [Strand]   12217..12317 [-]
Host baterium   Alcaligenes faecalis strain AN70

Cargo genes

Drug resistance gene   sul1, blaNDM-14, qacE, blaOXA-101, aac(6')-Ib, dfrA14, mph(E), msr(E)
Virulence gene   trwD, trwF, trwG, trwH1, trwK, trwM
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -