Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113992 |
| Name | oriT_p138R  |
| Organism | Achromobacter ruhlandii strain 138R |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MK423762 (16689..16799 [-], 111 nt) |
| oriT length | 111 nt |
| IRs (inverted repeats) | 71..78, 81..88 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 111 nt
>oriT_p138R
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8987 | GenBank | WP_033945278 |
| Name | traI_HTW17_RS00125_p138R |
UniProt ID | A0A482F307 |
| Length | 609 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 609 a.a. Molecular weight: 66706.47 Da Isoelectric Point: 10.6661
>WP_033945278.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICAGLGFAEHQRVSAVHTDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCATLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQATPARRSYQKGRPMRLRGGLD
TTELYARYKGAQQNLTAARAGVLAEAKRRKDRAIENAKKAAATRRAAIKHLTDGRLTKKLLYSQASAALR
ADLEAIHKRHDQERAAIYNEYRRRPWADWLKQQATAGDGQALDALRAREAAHGLKGDTIQGDGPAKPGHA
PVIDNITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRSGVPPVGQEPPPHRRNGLHTLGQLDRLHIEGEARRPAQAP
TQATPTPPAAKVQQPARPAPSELEQREARLRAQMEAKKAKRQEGKKRGR
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICAGLGFAEHQRVSAVHTDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCATLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQATPARRSYQKGRPMRLRGGLD
TTELYARYKGAQQNLTAARAGVLAEAKRRKDRAIENAKKAAATRRAAIKHLTDGRLTKKLLYSQASAALR
ADLEAIHKRHDQERAAIYNEYRRRPWADWLKQQATAGDGQALDALRAREAAHGLKGDTIQGDGPAKPGHA
PVIDNITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRSGVPPVGQEPPPHRRNGLHTLGQLDRLHIEGEARRPAQAP
TQATPTPPAAKVQQPARPAPSELEQREARLRAQMEAKKAKRQEGKKRGR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A482F307 |
Auxiliary protein
| ID | 5241 | GenBank | WP_011997489 |
| Name | WP_011997489_p138R |
UniProt ID | A0A482F300 |
| Length | 125 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 125 a.a. Molecular weight: 13548.38 Da Isoelectric Point: 8.9596
>WP_011997489.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Pseudomonadota]
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGRS
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGRS
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A482F300 |
Host bacterium
| ID | 14427 | GenBank | NZ_MK423762 |
| Plasmid name | p138R | Incompatibility group | - |
| Plasmid size | 34096 bp | Coordinate of oriT [Strand] | 16689..16799 [-] |
| Host baterium | Achromobacter ruhlandii strain 138R |
Cargo genes
| Drug resistance gene | sul1 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |