Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 113971
• Name: oriT_pWBG731
• Organism: Staphylococcus aureus strain WBG10514
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MH587574 (15709..15853 [+], 145 nt)
• oriT length: 145 nt
• IRs (inverted repeats): 105..110, 114..119 (TTTAAA..TTTAAA) 33..39, 44..50 (TGTCACA..TGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 145 nt

>oriT_pWBG731
TGTCACAAAAATAAGACAAATTCTACATGTTGTGTCACAAAACTGTGACAAACACAATATATAGTGTCACAAAACTGTGACACTATAGTTTATTGTGGGGTCACTTTAAATTCTTTAAAAACCTTGAAATGGCTGGCTTTGCCAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_HTT44_RS00095_pWBG731

• ID: 8973
• GenBank: WP_223223918
• Name: mobP2_HTT44_RS00095_pWBG731
• UniProt ID: _
• Length: 383 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 383 a.a.
• Molecular weight: 45796.08 Da
• Isoelectric Point: 9.9143

>WP_223223918.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MMPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAI
NKIKSDFSHAQKNKGMMWKDVISFDNSALESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVW
SGAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDE
SEPCKDKGMKKQIKKVHKLLEDIPRNQWNYNNNILKYIRPEIDKITDIYIKNYHSKDFEVFKNDLKRQTN
LYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQSTHSKSNATITKLKIGKCLNQA
LYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (2-358)

Protein structure

No available structure.

T4CP

t4cp2_HTT44_RS00045_pWBG731

• ID: 10411
• GenBank: WP_042853329
• Name: t4cp2_HTT44_RS00045_pWBG731
• UniProt ID: _
• Length: 924 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 924 a.a.
• Molecular weight: 106282.13 Da
• Isoelectric Point: 7.5736

>WP_042853329.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MRFGSKDKHGYRRKKLSFSQNKHLLAHPCILVSTLLLIMLLATAIANFLINILMTVFFGNHDIFTNMKQL
NTKYTDYIFNFRSDAVLLYIPIWGLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKAIPQR
EHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFATTFFS
KREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRGYLVE
IFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMILALC
EETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATVQAAP
DKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENGIEYE
EVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDGNNQVITSYFYVDHIDDKGQVTFNQKGELDNSDIT
IRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNMPPIP
NLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQITVH
SRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMKYRYQ
YLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKRIEEMQEGIDGQKEQENKNINDINQYKQSNE
EGNVAVRVKRLETLKEKVGDAIFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVSADNEVKEEEKTKR
IEMIQKFIKEAKTT

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (209-458)

• TraM recognition site of TraD and TraG (TraG-D_C) (615-732)

Protein structure

No available structure.

Host bacterium

• ID: 14406
• GenBank: NZ_MH587574
• Plasmid name: pWBG731
• Incompatibility group: -
• Plasmid size: 72158 bp
• Coordinate of oriT [Strand]: 15709..15853 [+]
• Host baterium: Staphylococcus aureus strain WBG10514

Cargo genes

• Drug resistance gene: blaZ, mupA
• Virulence gene: -
• Metal resistance gene: cadC
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21