Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113938 |
| Name | oriT_pLraf_19_4S_4  |
| Organism | Lactococcus raffinolactis strain Lr_19_4S |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP050538 (4194..4330 [-], 137 nt) |
| oriT length | 137 nt |
| IRs (inverted repeats) | 21..28, 42..49 (AAAAGGGG..CCCCTTTT) 25..30, 39..44 (GGGGAA..TTCCCC) |
| Location of nic site | 104..105 |
| Conserved sequence flanking the nic site |
GCTTGCAGTA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 137 nt
>oriT_pLraf_19_4S_4
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTTTTGATTTATATTTGCTATTTTGTT
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTTTTGATTTATATTTGCTATTTTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8952 | GenBank | WP_172507179 |
| Name | Relaxase_GU335_RS12210_pLraf_19_4S_4 |
UniProt ID | _ |
| Length | 410 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 410 a.a. Molecular weight: 47664.35 Da Isoelectric Point: 8.6375
>WP_172507179.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus raffinolactis]
MATIAKISNGASAASAINYALGHDRPMHEKTEQWLQDNQLERPVELAKCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALNELNPKIQNDWQKANDLGVELAEKLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREKKGESVQRARELNDQIAFRENWYILEPPKERQTDTEKELLAKNEYSYMDDLRG
RINQSLQDVSVSSYEAFKERLSTYGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
TRQNEFSAFEQREPTITPLERDTQQRESEIVSLEQAIEPRKSESLKRESTINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL
MATIAKISNGASAASAINYALGHDRPMHEKTEQWLQDNQLERPVELAKCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALNELNPKIQNDWQKANDLGVELAEKLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREKKGESVQRARELNDQIAFRENWYILEPPKERQTDTEKELLAKNEYSYMDDLRG
RINQSLQDVSVSSYEAFKERLSTYGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
TRQNEFSAFEQREPTITPLERDTQQRESEIVSLEQAIEPRKSESLKRESTINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 14373 | GenBank | NZ_CP050538 |
| Plasmid name | pLraf_19_4S_4 | Incompatibility group | - |
| Plasmid size | 10031 bp | Coordinate of oriT [Strand] | 4194..4330 [-] |
| Host baterium | Lactococcus raffinolactis strain Lr_19_4S |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |