Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113923
Name   oriT_pPM64421a in_silico
Organism   Proteus mirabilis strain A64421
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MF150118 (43751..43901 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pPM64421a
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8938 GenBank   WP_001337757
Name   mobH_PM64421a_RS00250_pPM64421a insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   10379 GenBank   WP_000178856
Name   traD_PM64421a_RS00255_pPM64421a insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   10380 GenBank   WP_000637386
Name   traC_PM64421a_RS00335_pPM64421a insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41835..67026

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PM64421a_RS00240 (PM64421a_00047) 37860..38093 - 234 WP_001191892 hypothetical protein -
PM64421a_RS00245 (PM64421a_00048) 38075..38692 - 618 WP_001249396 hypothetical protein -
PM64421a_RS00250 (PM64421a_00049) 38860..41838 + 2979 WP_001337757 MobH family relaxase -
PM64421a_RS00255 (PM64421a_00050) 41835..43700 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
PM64421a_RS00260 (PM64421a_00051) 43711..44295 + 585 WP_000332866 hypothetical protein -
PM64421a_RS00265 (PM64421a_00052) 44252..44881 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
PM64421a_RS00270 (PM64421a_00053) 44891..45337 + 447 WP_000122506 hypothetical protein -
PM64421a_RS00275 (PM64421a_00054) 45347..45724 + 378 WP_000869261 hypothetical protein -
PM64421a_RS00280 (PM64421a_00055) 45724..46386 + 663 WP_001231463 hypothetical protein -
PM64421a_RS00285 (PM64421a_00056) 46567..46710 + 144 WP_001275801 hypothetical protein -
PM64421a_RS00290 (PM64421a_00057) 46722..47087 + 366 WP_001052531 hypothetical protein -
PM64421a_RS00295 (PM64421a_00058) 47232..47513 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
PM64421a_RS00300 (PM64421a_00059) 47510..48136 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
PM64421a_RS00305 (PM64421a_00060) 48120..49037 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
PM64421a_RS00310 (PM64421a_00061) 49037..50350 + 1314 WP_024131605 TraB/VirB10 family protein traB
PM64421a_RS00315 (PM64421a_00062) 50347..50925 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
PM64421a_RS00320 (PM64421a_00063) 50929..51321 + 393 WP_000479535 TraA family conjugative transfer protein -
PM64421a_RS00325 (PM64421a_00064) 51522..57053 + 5532 WP_085281193 Ig-like domain-containing protein -
PM64421a_RS00330 (PM64421a_00065) 57202..57909 + 708 WP_001259347 DsbC family protein -
PM64421a_RS00335 (PM64421a_00066) 57906..60353 + 2448 WP_000637386 type IV secretion system protein TraC virb4
PM64421a_RS00340 (PM64421a_00067) 60368..60685 + 318 WP_000351984 hypothetical protein -
PM64421a_RS00345 (PM64421a_00068) 60682..61212 + 531 WP_001010738 S26 family signal peptidase -
PM64421a_RS00350 (PM64421a_00069) 61175..62440 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
PM64421a_RS00355 (PM64421a_00070) 62437..63108 + 672 WP_001337754 EAL domain-containing protein -
PM64421a_RS00360 (PM64421a_00071) 63108..64112 + 1005 WP_043940352 TraU family protein traU
PM64421a_RS00365 (PM64421a_00072) 64228..67026 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
PM64421a_RS00370 (PM64421a_00073) 67065..67925 - 861 WP_000709517 hypothetical protein -
PM64421a_RS00375 (PM64421a_00074) 68048..68689 - 642 WP_000796664 hypothetical protein -
PM64421a_RS00380 (PM64421a_00075) 68983..69303 + 321 WP_000547566 hypothetical protein -
PM64421a_RS00385 (PM64421a_00076) 69607..69792 + 186 WP_001186917 hypothetical protein -
PM64421a_RS00390 (PM64421a_00077) 70012..70980 + 969 WP_000085162 AAA family ATPase -
PM64421a_RS00395 (PM64421a_00078) 70991..71899 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   14358 GenBank   NZ_MF150118
Plasmid name   pPM64421a Incompatibility group   IncA/C2
Plasmid size   176346 bp Coordinate of oriT [Strand]   43751..43901 [+]
Host baterium   Proteus mirabilis strain A64421

Cargo genes

Drug resistance gene   blaTEM-1B, rmtB, tet(G), floR, qacE, aadA2, dfrA12, aac(3)-IId, aph(6)-Id, aph(3'')-Ib, sul2, blaCTX-M-14, erm(42)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -