Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113917
Name   oriT_pMRVIM0713 in_silico
Organism   Pseudomonas aeruginosa strain MRSN17623
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KP975076 (35845..35955 [+], 111 nt)
oriT length   111 nt
IRs (inverted repeats)      71..78, 81..88  (CACCATGC..GCATGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 111 nt

>oriT_pMRVIM0713
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8935 GenBank   WP_033945278
Name   traI_HTF13_RS00230_pMRVIM0713 insolico UniProt ID   A0A482F307
Length   609 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 609 a.a.        Molecular weight: 66706.47 Da        Isoelectric Point: 10.6661

>WP_033945278.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICAGLGFAEHQRVSAVHTDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCATLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQATPARRSYQKGRPMRLRGGLD
TTELYARYKGAQQNLTAARAGVLAEAKRRKDRAIENAKKAAATRRAAIKHLTDGRLTKKLLYSQASAALR
ADLEAIHKRHDQERAAIYNEYRRRPWADWLKQQATAGDGQALDALRAREAAHGLKGDTIQGDGPAKPGHA
PVIDNITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRSGVPPVGQEPPPHRRNGLHTLGQLDRLHIEGEARRPAQAP
TQATPTPPAAKVQQPARPAPSELEQREARLRAQMEAKKAKRQEGKKRGR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(12-250)

Large polyvalent protein-associated domain 7 (LPD7)

(422-511)


  Protein structure


Source ID Structure
AlphaFold DB A0A482F307


Auxiliary protein

ID   631 GenBank   WP_011997489
Name   WP_011997489_pMRVIM0713 insolico UniProt ID   A0A482F300
Length   125 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 125 a.a.        Molecular weight: 13548.38 Da        Isoelectric Point: 8.9596

>WP_011997489.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Pseudomonadota]
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGRS

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A482F300


Host bacterium

ID   14352 GenBank   NZ_KP975076
Plasmid name   pMRVIM0713 Incompatibility group   -
Plasmid size   36032 bp Coordinate of oriT [Strand]   35845..35955 [+]
Host baterium   Pseudomonas aeruginosa strain MRSN17623

Cargo genes

Drug resistance gene   aph(3'')-Ib, aph(6)-Id, cmx, sul1, qacE, blaOXA-10, blaVIM-6
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -