Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 113897
• Name: oriT_pE512
• Organism: Enterococcus faecalis strain E512
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MG765452 (7949..7998 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pE512
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_HTQ42_RS00070_pE512

• ID: 8923
• GenBank: WP_002415208
• Name: Relaxase_HTQ42_RS00070_pE512
• UniProt ID: Q7X5P3
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65380.01 Da
• Isoelectric Point: 6.2230

>WP_002415208.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGKYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

• AlphaFold DB: Q7X5P3

Auxiliary protein

WP_002393737_pE512

• ID: 5228
• GenBank: WP_002393737
• Name: WP_002393737_pE512
• UniProt ID: A0A8E7CBI6
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13965.01 Da
• Isoelectric Point: 9.6545

>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

No available structure.

T4CP

t4cp2_HTQ42_RS00035_pE512

• ID: 10362
• GenBank: WP_002367691
• Name: t4cp2_HTQ42_RS00035_pE512
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70145.14 Da
• Isoelectric Point: 6.1807

>WP_002367691.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 34196..48666

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HTQ42_RS00210	29527..29907	+	381	WP_002367664	hypothetical protein	-
HTQ42_RS00215	29958..30179	+	222	WP_002386480	hypothetical protein	-
HTQ42_RS00220	30481..30819	+	339	WP_229022143	hypothetical protein	-
HTQ42_RS00225	30749..33427	+	2679	WP_087548818	surface exclusion protein SEA1/PrgA	-
HTQ42_RS00230	33571..33906	+	336	WP_002360910	DUF5406 domain-containing protein	-
HTQ42_RS00235	34196..38113	+	3918	WP_087548819	LPXTG-anchored aggregation substance PrgB/Asc10	prgB
HTQ42_RS00240	38453..38593	+	141	WP_113826368	LPXTG cell wall anchor domain-containing protein	prgC
HTQ42_RS00245	38656..39012	+	357	WP_010711024	pheromone response system RNA-binding regulator PrgU	-
HTQ42_RS00250	39040..39897	+	858	WP_126254887	pheromone response LPXTG-anchored adhesin PrgC	prgC
HTQ42_RS00255	39939..40838	+	900	WP_172692903	type III secretion system protein PrgD	-
HTQ42_RS00260	40858..41292	+	435	WP_002365857	hypothetical protein	-
HTQ42_RS00265	41339..41566	+	228	WP_002365858	hypothetical protein	-
HTQ42_RS00270	41579..41875	+	297	WP_128701256	type III secretion system protein PrgG	-
HTQ42_RS00275	41890..42690	+	801	WP_010774271	type IV secretion system protein	prgHb
HTQ42_RS00280	42692..43045	+	354	WP_002367919	PrgI family mobile element protein	prgIc
HTQ42_RS00285	42999..45389	+	2391	WP_172692904	VirB4-like conjugal transfer ATPase, CD1110 family	virb4
HTQ42_RS00290	45401..48016	+	2616	WP_128701255	phage tail tip lysozyme	prgK
HTQ42_RS00295	48040..48666	+	627	WP_128701254	hypothetical protein	prgL

Host bacterium

• ID: 14332
• GenBank: NZ_MG765452
• Plasmid name: pE512
• Incompatibility group: -
• Plasmid size: 48881 bp
• Coordinate of oriT [Strand]: 7949..7998 [+]
• Host baterium: Enterococcus faecalis strain E512

Cargo genes

• Drug resistance gene: -
• Virulence gene: prgB/asc10, EF0485
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -