Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113895 |
| Name | oriT_MED|unnamed  |
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain MED |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MH401970 (12564..12664 [-], 101 nt) |
| oriT length | 101 nt |
| IRs (inverted repeats) | 80..85, 91..96 (AAAAAA..TTTTTT) 20..26, 38..44 (TAAATCA..TGATTTA) |
| Location of nic site | 62..63 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 101 nt
>oriT_MED|unnamed
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8921 | GenBank | WP_113349826 |
| Name | mobF_HTS73_RS00105_MED|unnamed |
UniProt ID | _ |
| Length | 1076 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1076 a.a. Molecular weight: 119875.04 Da Isoelectric Point: 6.5510
>WP_113349826.1 MULTISPECIES: MobF family relaxase [Enterobacteriaceae]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 5227 | GenBank | WP_001749975 |
| Name | WP_001749975_MED|unnamed |
UniProt ID | A0A5C2CVS6 |
| Length | 138 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 138 a.a. Molecular weight: 15332.60 Da Isoelectric Point: 5.9670
>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5C2CVS6 |
T4CP
| ID | 10360 | GenBank | WP_000342688 |
| Name | t4cp2_HTS73_RS00100_MED|unnamed |
UniProt ID | _ |
| Length | 509 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 509 a.a. Molecular weight: 57762.87 Da Isoelectric Point: 9.6551
>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 28486..38811
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTS73_RS00145 | 24091..25020 | + | 930 | WP_003150544 | recombinase family protein | - |
| HTS73_RS00150 | 25060..25269 | - | 210 | Protein_29 | DUF6710 family protein | - |
| HTS73_RS00155 | 25410..25955 | + | 546 | WP_001493763 | plasmid pRiA4b ORF-3 family protein | - |
| HTS73_RS00160 | 26092..26664 | + | 573 | WP_110293154 | recombinase family protein | - |
| HTS73_RS00165 | 26701..28092 | + | 1392 | WP_172693115 | ISKra4-like element ISKpn19 family transposase | - |
| HTS73_RS00170 | 28124..28486 | - | 363 | Protein_33 | phospholipase D-like domain-containing protein | - |
| HTS73_RS00175 | 28486..29481 | - | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTS73_RS00180 | 29523..30683 | - | 1161 | WP_000101710 | type IV secretion system protein VirB10 | virB10 |
| HTS73_RS00185 | 30683..31567 | - | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTS73_RS00190 | 31578..32276 | - | 699 | WP_000646594 | virB8 family protein | virB8 |
| HTS73_RS00195 | 32266..32424 | - | 159 | WP_012561180 | hypothetical protein | - |
| HTS73_RS00200 | 32495..33535 | - | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
| HTS73_RS00205 | 33551..33778 | - | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| HTS73_RS00210 | 33786..34550 | - | 765 | WP_013149461 | type IV secretion system protein | virB5 |
| HTS73_RS00215 | 34517..37117 | - | 2601 | WP_001749961 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| HTS73_RS00220 | 37117..37434 | - | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| HTS73_RS00225 | 37484..37777 | - | 294 | WP_001749962 | hypothetical protein | virB2 |
| HTS73_RS00230 | 37787..38068 | - | 282 | WP_000440698 | transcriptional repressor KorA | - |
| HTS73_RS00235 | 38077..38811 | - | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
| HTS73_RS00240 | 38854..39225 | + | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| HTS73_RS00245 | 39241..39585 | + | 345 | WP_001749964 | hypothetical protein | - |
| HTS73_RS00250 | 39582..39896 | + | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTS73_RS00255 | 40010..40243 | + | 234 | WP_001191790 | hypothetical protein | - |
| HTS73_RS00260 | 40293..40940 | + | 648 | WP_015344958 | restriction endonuclease | - |
| HTS73_RS00265 | 40945..41151 | + | 207 | WP_001749967 | hypothetical protein | - |
| HTS73_RS00270 | 41162..41434 | - | 273 | Protein_53 | IS1 family transposase | - |
| HTS73_RS00275 | 41533..42966 | + | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
Host bacterium
| ID | 14330 | GenBank | NZ_MH401970 |
| Plasmid name | MED|unnamed | Incompatibility group | IncN |
| Plasmid size | 50392 bp | Coordinate of oriT [Strand] | 12564..12664 [-] |
| Host baterium | Enterobacter hormaechei subsp. steigerwaltii strain MED |
Cargo genes
| Drug resistance gene | aac(6')-IIa, blaTMB-1, dfrA14 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |