Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113892 |
| Name | oriT_p30860-NR  |
| Organism | Enterobacter cloacae strain 30860 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MF344556 (39485..40079 [+], 595 nt) |
| oriT length | 595 nt |
| IRs (inverted repeats) | 569..574, 579..584 (ATGCCC..GGGCAT) 497..503, 508..514 (CCTCCCG..CGGGAGG) 433..438, 442..447 (GTTCGC..GCGAAC) 75..80, 86..91 (TGCTTT..AAAGCA) 69..74, 82..87 (TTACTT..AAGTAA) |
| Location of nic site | 294..295 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 595 nt
>oriT_p30860-NR
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGTAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGTAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8918 | GenBank | WP_139003215 |
| Name | mobP1_HTO11_RS00215_p30860-NR |
UniProt ID | _ |
| Length | 386 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 386 a.a. Molecular weight: 44400.94 Da Isoelectric Point: 10.6293
>WP_139003215.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVSVDKEYRFKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGKKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
MGVSVDKEYRFKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGKKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26960..36915
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTO11_RS00125 | 22519..23292 | - | 774 | WP_044715185 | hypothetical protein | - |
| HTO11_RS00130 | 23307..23777 | - | 471 | WP_044715183 | thermonuclease family protein | - |
| HTO11_RS00135 | 23774..24094 | - | 321 | WP_044715215 | hypothetical protein | - |
| HTO11_RS00140 | 24115..24318 | - | 204 | WP_044715181 | hypothetical protein | - |
| HTO11_RS00260 | 24387..24725 | - | 339 | WP_225622178 | hypothetical protein | - |
| HTO11_RS00150 | 24730..25125 | - | 396 | WP_044715177 | cag pathogenicity island Cag12 family protein | - |
| HTO11_RS00155 | 25122..26957 | - | 1836 | Protein_31 | type IV secretory system conjugative DNA transfer family protein | - |
| HTO11_RS00160 | 26960..27991 | - | 1032 | WP_044715211 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTO11_RS00165 | 28015..29226 | - | 1212 | WP_044715172 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| HTO11_RS00170 | 29223..30152 | - | 930 | WP_044715170 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTO11_RS00175 | 30157..30885 | - | 729 | WP_139003213 | virB8 family protein | virB8 |
| HTO11_RS00180 | 31125..32180 | - | 1056 | WP_044715165 | type IV secretion system protein | virB6 |
| HTO11_RS00185 | 32192..32449 | - | 258 | WP_044715163 | EexN family lipoprotein | - |
| HTO11_RS00190 | 32459..33205 | - | 747 | WP_044715162 | type IV secretion system protein | - |
| HTO11_RS00195 | 33216..35975 | - | 2760 | WP_044715159 | VirB3 family type IV secretion system protein | virb4 |
| HTO11_RS00200 | 35997..36287 | - | 291 | WP_044715156 | TrbC/VirB2 family protein | virB2 |
| HTO11_RS00205 | 36271..36915 | - | 645 | WP_044715155 | lytic transglycosylase domain-containing protein | virB1 |
| HTO11_RS00210 | 37139..37630 | - | 492 | WP_044715152 | transcription termination/antitermination NusG family protein | - |
| HTO11_RS00215 | 37986..39146 | - | 1161 | WP_139003215 | MobP1 family relaxase | - |
| HTO11_RS00220 | 39149..39697 | - | 549 | WP_044715148 | DNA distortion polypeptide 1 | - |
| HTO11_RS00225 | 40025..40282 | - | 258 | WP_044715147 | hypothetical protein | - |
| HTO11_RS00230 | 40298..40447 | - | 150 | WP_162852120 | hypothetical protein | - |
| HTO11_RS00235 | 40525..40866 | - | 342 | WP_059347451 | hypothetical protein | - |
| HTO11_RS00240 | 40963..41202 | - | 240 | WP_044715143 | hypothetical protein | - |
| HTO11_RS00245 | 41195..41476 | - | 282 | WP_048235114 | hypothetical protein | - |
Host bacterium
| ID | 14327 | GenBank | NZ_MF344556 |
| Plasmid name | p30860-NR | Incompatibility group | IncX5 |
| Plasmid size | 44121 bp | Coordinate of oriT [Strand] | 39485..40079 [+] |
| Host baterium | Enterobacter cloacae strain 30860 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |