Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113887
Name   oriT_R222 in_silico
Organism   Enterobacter cloacae strain 20130723
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX869741 (52337..52487 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_R222
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8913 GenBank   WP_001337757
Name   mobH_HTK72_RS00390_R222 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   10354 GenBank   WP_000178856
Name   traD_HTK72_RS00395_R222 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   10355 GenBank   WP_000637386
Name   traC_HTK72_RS00475_R222 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50421..75612

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTK72_RS00380 46446..46679 - 234 WP_001191892 hypothetical protein -
HTK72_RS00385 46661..47278 - 618 WP_001249396 hypothetical protein -
HTK72_RS00390 47446..50424 + 2979 WP_001337757 MobH family relaxase -
HTK72_RS00395 50421..52286 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
HTK72_RS00400 52297..52881 + 585 WP_000332866 hypothetical protein -
HTK72_RS00405 52838..53467 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
HTK72_RS00410 53477..53923 + 447 WP_000122506 hypothetical protein -
HTK72_RS00415 53933..54310 + 378 WP_000869261 hypothetical protein -
HTK72_RS00420 54310..54972 + 663 WP_001231463 hypothetical protein -
HTK72_RS00425 55153..55296 + 144 WP_001275801 hypothetical protein -
HTK72_RS00430 55308..55673 + 366 WP_001052531 hypothetical protein -
HTK72_RS00435 55818..56099 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HTK72_RS00440 56096..56722 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
HTK72_RS00445 56706..57623 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HTK72_RS00450 57623..58936 + 1314 WP_024131605 TraB/VirB10 family protein traB
HTK72_RS00455 58933..59511 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HTK72_RS00460 59515..59907 + 393 WP_000479535 TraA family conjugative transfer protein -
HTK72_RS00465 60108..65639 + 5532 WP_000606833 Ig-like domain-containing protein -
HTK72_RS00470 65788..66495 + 708 WP_001259347 DsbC family protein -
HTK72_RS00475 66492..68939 + 2448 WP_000637386 type IV secretion system protein TraC virb4
HTK72_RS00480 68954..69271 + 318 WP_000351984 hypothetical protein -
HTK72_RS00485 69268..69798 + 531 WP_001010738 S26 family signal peptidase -
HTK72_RS00490 69761..71026 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
HTK72_RS00495 71023..71694 + 672 WP_001337754 EAL domain-containing protein -
HTK72_RS00500 71694..72698 + 1005 WP_043940352 TraU family protein traU
HTK72_RS00505 72814..75612 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
HTK72_RS00510 75651..76511 - 861 WP_000709517 hypothetical protein -
HTK72_RS00515 76634..77275 - 642 WP_000796664 hypothetical protein -
HTK72_RS00520 77569..77889 + 321 WP_000547566 hypothetical protein -
HTK72_RS00525 78193..78378 + 186 WP_001186917 hypothetical protein -
HTK72_RS00530 78598..79566 + 969 WP_000085162 AAA family ATPase -
HTK72_RS00535 79577..80485 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   14322 GenBank   NZ_KX869741
Plasmid name   R222 Incompatibility group   IncA/C2
Plasmid size   177037 bp Coordinate of oriT [Strand]   52337..52487 [+]
Host baterium   Enterobacter cloacae strain 20130723

Cargo genes

Drug resistance gene   sul2, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, blaOXA-427
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -