Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113885 |
| Name | oriT_pT5282-CTXM  |
| Organism | Enterobacter cloacae strain T5282 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MF062700 (55570..56164 [+], 595 nt) |
| oriT length | 595 nt |
| IRs (inverted repeats) | 569..574, 579..584 (ATGCCC..GGGCAT) 497..503, 508..514 (CCTCCCG..CGGGAGG) 433..438, 442..447 (GTTCGC..GCGAAC) 75..80, 86..91 (TGCTTT..AAAGCA) 69..74, 82..87 (TTACTT..AAGTAA) |
| Location of nic site | 294..295 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 595 nt
>oriT_pT5282-CTXM
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8911 | GenBank | WP_044864205 |
| Name | mobP1_HTN76_RS00290_pT5282-CTXM |
UniProt ID | A0A221ZPJ2 |
| Length | 386 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 386 a.a. Molecular weight: 44352.85 Da Isoelectric Point: 10.6126
>WP_044864205.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVSVDKEYRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
MGVSVDKEYRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A221ZPJ2 |
T4CP
| ID | 10351 | GenBank | WP_044715174 |
| Name | t4cp2_HTN76_RS00230_pT5282-CTXM |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69245.98 Da Isoelectric Point: 7.1493
>WP_044715174.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43045..53000
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTN76_RS00200 (pT5282-CTXM_42) | 38604..39377 | - | 774 | WP_044715185 | hypothetical protein | - |
| HTN76_RS00205 (pT5282-CTXM_43) | 39392..39862 | - | 471 | WP_044715183 | thermonuclease family protein | - |
| HTN76_RS00210 (pT5282-CTXM_44) | 39859..40179 | - | 321 | WP_044715215 | hypothetical protein | - |
| HTN76_RS00215 (pT5282-CTXM_45) | 40200..40403 | - | 204 | WP_044715181 | hypothetical protein | - |
| HTN76_RS00335 (pT5282-CTXM_46) | 40472..40810 | - | 339 | WP_225622178 | hypothetical protein | - |
| HTN76_RS00225 (pT5282-CTXM_47) | 40815..41210 | - | 396 | WP_044715177 | cag pathogenicity island Cag12 family protein | - |
| HTN76_RS00230 (pT5282-CTXM_48) | 41207..43042 | - | 1836 | WP_044715174 | type IV secretory system conjugative DNA transfer family protein | - |
| HTN76_RS00235 (pT5282-CTXM_49) | 43045..44076 | - | 1032 | WP_172691582 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTN76_RS00240 (pT5282-CTXM_50) | 44100..45311 | - | 1212 | WP_044715172 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| HTN76_RS00245 (pT5282-CTXM_51) | 45308..46237 | - | 930 | WP_044715170 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTN76_RS00250 (pT5282-CTXM_52) | 46242..46970 | - | 729 | WP_044715168 | virB8 family protein | virB8 |
| HTN76_RS00255 (pT5282-CTXM_53) | 47210..48265 | - | 1056 | WP_044715165 | type IV secretion system protein | virB6 |
| HTN76_RS00260 (pT5282-CTXM_54) | 48277..48534 | - | 258 | WP_044715163 | EexN family lipoprotein | - |
| HTN76_RS00265 (pT5282-CTXM_55) | 48544..49290 | - | 747 | WP_044715162 | type IV secretion system protein | - |
| HTN76_RS00270 (pT5282-CTXM_56) | 49301..52060 | - | 2760 | WP_044715159 | VirB3 family type IV secretion system protein | virb4 |
| HTN76_RS00275 (pT5282-CTXM_57) | 52082..52372 | - | 291 | WP_044715156 | TrbC/VirB2 family protein | virB2 |
| HTN76_RS00280 (pT5282-CTXM_58) | 52356..53000 | - | 645 | WP_172691580 | lytic transglycosylase domain-containing protein | virB1 |
| HTN76_RS00285 (pT5282-CTXM_60) | 53224..53715 | - | 492 | WP_044715152 | transcription termination/antitermination NusG family protein | - |
| HTN76_RS00290 (pT5282-CTXM_61) | 54071..55231 | - | 1161 | WP_044864205 | MobP1 family relaxase | - |
| HTN76_RS00295 (pT5282-CTXM_62) | 55234..55782 | - | 549 | WP_044715148 | DNA distortion polypeptide 1 | - |
| HTN76_RS00300 (pT5282-CTXM_63) | 56110..56367 | - | 258 | WP_044715147 | hypothetical protein | - |
| HTN76_RS00305 (pT5282-CTXM_64) | 56383..56532 | - | 150 | WP_162852120 | hypothetical protein | - |
| HTN76_RS00310 (pT5282-CTXM_65) | 56610..56951 | - | 342 | WP_059347451 | hypothetical protein | - |
| HTN76_RS00315 (pT5282-CTXM_66) | 57048..57287 | - | 240 | WP_044715143 | hypothetical protein | - |
| HTN76_RS00320 (pT5282-CTXM_67) | 57280..57561 | - | 282 | WP_048235114 | hypothetical protein | - |
Host bacterium
| ID | 14320 | GenBank | NZ_MF062700 |
| Plasmid name | pT5282-CTXM | Incompatibility group | IncX5 |
| Plasmid size | 60206 bp | Coordinate of oriT [Strand] | 55570..56164 [+] |
| Host baterium | Enterobacter cloacae strain T5282 |
Cargo genes
| Drug resistance gene | blaTEM-1B, blaCTX-M-3 |
| Virulence gene | manB, manC |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |