Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113884
Name   oriT_pMRVIM0813 in_silico
Organism   Enterobacter cloacae strain MRSN17626
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KP975077 (289883..290137 [+], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pMRVIM0813
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8910 GenBank   WP_012477394
Name   mobH_HTF87_RS01560_pMRVIM0813 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   10350 GenBank   WP_001284073
Name   traD_HTF87_RS01555_pMRVIM0813 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 197965..207172

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTF87_RS01060 195255..195416 - 162 WP_001371949 hypothetical protein -
HTF87_RS01065 197965..200646 - 2682 WP_000387412 TraC family protein virb4
HTF87_RS01070 200655..201605 - 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
HTF87_RS01075 201615..202367 - 753 WP_182911723 protein-disulfide isomerase HtdT -
HTF87_RS01080 202486..202968 - 483 WP_000377632 hypothetical protein -
HTF87_RS01085 202976..204331 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
HTF87_RS01090 204321..204782 - 462 WP_000521242 plasmid transfer protein HtdO -
HTF87_RS01095 204784..206055 - 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
HTF87_RS01100 206055..206843 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
HTF87_RS01105 206855..207205 - 351 WP_001447770 type IV conjugative transfer system protein TraL -
HTF87_RS01110 207223..207576 - 354 WP_000423602 hypothetical protein -
HTF87_RS01115 208725..209600 - 876 WP_000594611 RepB family plasmid replication initiator protein -
HTF87_RS01120 210077..211129 + 1053 WP_001065778 hypothetical protein -
HTF87_RS01125 211149..211349 + 201 WP_000594930 hypothetical protein -
HTF87_RS01130 211346..212062 + 717 WP_000838219 zinc finger-like domain-containing protein -

Host bacterium

ID   14319 GenBank   NZ_KP975077
Plasmid name   pMRVIM0813 Incompatibility group   IncHI2A
Plasmid size   311662 bp Coordinate of oriT [Strand]   289883..290137 [+]
Host baterium   Enterobacter cloacae strain MRSN17626

Cargo genes

Drug resistance gene   mcr-9, blaCTX-M-9, sul1, qacE, aadA2, dfrA16, catA1, blaSHV-12, tet(A), aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, catB2, ant(3'')-Ia, aph(3')-XV, blaVIM-1
Virulence gene   -
Metal resistance gene   arsH, arsR, arsB, arsC, rcnR/yohL, ncrC, pcoE, pcoS, merR, merT, merP, merC, merA, merD, merE, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -