Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113882
Name   oriT_pJF-786 in_silico
Organism   Enterobacter cloacae strain H140960786
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX912255 (5913..6050 [-], 138 nt)
oriT length   138 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 138 nt

>oriT_pJF-786
CGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGCCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8909 GenBank   WP_172689005
Name   traI_HTK08_RS00055_pJF-786 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101295.82 Da        Isoelectric Point: 9.8369

>WP_172689005.1 TraI/MobA(P) family conjugative relaxase [Enterobacter cloacae]
MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG
FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIE
DGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQ
QVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWNGKGE
VLNAMRSVVAAEQAAEKAALKEKHKKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA
SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKLE
RLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRK
PKHQREDGSYPEVRLLKVERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(17-272)

Large polyvalent protein-associated domain 7 (LPD7)

(438-526)

RepB DNA-primase from phage plasmid (RepB_primase)

(548-806)


  Protein structure



No available structure.




Host bacterium

ID   14317 GenBank   NZ_KX912255
Plasmid name   pJF-786 Incompatibility group   -
Plasmid size   25354 bp Coordinate of oriT [Strand]   5913..6050 [-]
Host baterium   Enterobacter cloacae strain H140960786

Cargo genes

Drug resistance gene   blaGES-5, dfrA14, qacE, sul1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -