Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113876 |
| Name | oriT_pRSM9152_2  |
| Organism | Klebsiella michiganensis strain 9152 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP129897 (7943..7991 [+], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_pRSM9152_2
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10344 | GenBank | WP_301718422 |
| Name | traD_Q0L46_RS30225_pRSM9152_2 |
UniProt ID | _ |
| Length | 767 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 767 a.a. Molecular weight: 85761.65 Da Isoelectric Point: 4.9170
>WP_301718422.1 type IV conjugative transfer system coupling protein TraD [Klebsiella michiganensis]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRTLDTRVDTRLSALLEAREAEGSLARALFTPDAPEPEQRDADSKTPVGQPAPESAP
VSPVPVKAGSTAETLAAPASVKAQEAPQAKVTTVPLTRPAPVATSAAATGATVSSVATTTATTGGTEQAL
TQQSAEQGQEMMPAGMTEDGEIEDMAAYDAWLSDEQTQRDMQRREEVNINHSHQRDEPDDIEFGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRTLDTRVDTRLSALLEAREAEGSLARALFTPDAPEPEQRDADSKTPVGQPAPESAP
VSPVPVKAGSTAETLAAPASVKAQEAPQAKVTTVPLTRPAPVATSAAATGATVSSVATTTATTGGTEQAL
TQQSAEQGQEMMPAGMTEDGEIEDMAAYDAWLSDEQTQRDMQRREEVNINHSHQRDEPDDIEFGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 70617..84482
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Q0L46_RS30225 (Q0L46_30225) | 70617..72920 | - | 2304 | WP_301718422 | type IV conjugative transfer system coupling protein TraD | virb4 |
| Q0L46_RS30230 (Q0L46_30230) | 73158..73694 | - | 537 | WP_301718420 | hypothetical protein | - |
| Q0L46_RS30235 (Q0L46_30235) | 73705..76548 | - | 2844 | WP_301718419 | conjugal transfer mating-pair stabilization protein TraG | traG |
| Q0L46_RS30240 (Q0L46_30240) | 76548..77918 | - | 1371 | WP_301718418 | conjugal transfer pilus assembly protein TraH | traH |
| Q0L46_RS30245 (Q0L46_30245) | 77905..78480 | - | 576 | WP_301718417 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| Q0L46_RS30250 (Q0L46_30250) | 78452..78691 | - | 240 | WP_301718416 | type-F conjugative transfer system pilin chaperone TraQ | - |
| Q0L46_RS30255 (Q0L46_30255) | 78702..79454 | - | 753 | WP_301718415 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| Q0L46_RS30260 (Q0L46_30260) | 79473..79799 | - | 327 | WP_301718414 | hypothetical protein | - |
| Q0L46_RS30265 (Q0L46_30265) | 79810..80037 | - | 228 | WP_064174417 | conjugal transfer protein TrbE | - |
| Q0L46_RS30270 (Q0L46_30270) | 80027..80308 | - | 282 | WP_064174416 | hypothetical protein | - |
| Q0L46_RS30275 (Q0L46_30275) | 80346..82226 | - | 1881 | WP_301718413 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| Q0L46_RS30280 (Q0L46_30280) | 82223..82840 | - | 618 | WP_064381378 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| Q0L46_RS30285 (Q0L46_30285) | 82853..83836 | - | 984 | WP_177342975 | conjugal transfer pilus assembly protein TraU | traU |
| Q0L46_RS30290 (Q0L46_30290) | 83856..84482 | - | 627 | WP_301743359 | type-F conjugative transfer system protein TraW | traW |
| Q0L46_RS30295 (Q0L46_30295) | 84479..84886 | - | 408 | WP_301743362 | type-F conjugative transfer system protein TrbI | - |
| Q0L46_RS30300 (Q0L46_30300) | 84919..86283 | - | 1365 | Protein_103 | DUF87 domain-containing protein | - |
Host bacterium
| ID | 14311 | GenBank | NZ_CP129897 |
| Plasmid name | pRSM9152_2 | Incompatibility group | IncFII |
| Plasmid size | 86283 bp | Coordinate of oriT [Strand] | 7943..7991 [+] |
| Host baterium | Klebsiella michiganensis strain 9152 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |