Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113875 |
| Name | oriT_pRSM9152_1  |
| Organism | Klebsiella michiganensis strain 9152 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP129896 (145135..145181 [-], 47 nt) |
| oriT length | 47 nt |
| IRs (inverted repeats) | 4..11, 14..21 (GCAAAATT..AATTTTGC) |
| Location of nic site | 30..31 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 47 nt
>oriT_pRSM9152_1
TCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
TCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10343 | GenBank | WP_301717990 |
| Name | traD_Q0L46_RS29010_pRSM9152_1 |
UniProt ID | _ |
| Length | 773 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 773 a.a. Molecular weight: 86636.63 Da Isoelectric Point: 4.8571
>WP_301717990.1 type IV conjugative transfer system coupling protein TraD [Klebsiella michiganensis]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVLAAVVSLVICIVTFFVASWVLGCQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGQASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPMDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKVAEGFIQRTFDTRVDARLNALLAAREAEGSLARMLFTPDAPEAEQADQDSKAGAQHEPVKQPE
TAEGTVSPVPVTALSAAKTPAAEDPVKSPETLRPEVSDTRITTVPLIRTKPASSATGTAAAAAGVTAHTG
GTEQELAQQSADVGQEMMPAGMNEDGEIEDMQAYDAWLADELTQRDMQRREEVNINHSHRRDEQDDIEIG
GNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVLAAVVSLVICIVTFFVASWVLGCQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGQASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPMDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKVAEGFIQRTFDTRVDARLNALLAAREAEGSLARMLFTPDAPEAEQADQDSKAGAQHEPVKQPE
TAEGTVSPVPVTALSAAKTPAAEDPVKSPETLRPEVSDTRITTVPLIRTKPASSATGTAAAAAGVTAHTG
GTEQELAQQSADVGQEMMPAGMNEDGEIEDMQAYDAWLADELTQRDMQRREEVNINHSHRRDEQDDIEIG
GNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1802..17198
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Q0L46_RS28925 (Q0L46_28925) | 1..1365 | + | 1365 | Protein_0 | DUF87 domain-containing protein | - |
| Q0L46_RS28930 (Q0L46_28930) | 1398..1805 | + | 408 | WP_049071729 | type-F conjugative transfer system protein TrbI | - |
| Q0L46_RS28935 (Q0L46_28935) | 1802..2428 | + | 627 | WP_301719595 | type-F conjugative transfer system protein TraW | traW |
| Q0L46_RS28940 (Q0L46_28940) | 2447..3430 | + | 984 | WP_265829634 | conjugal transfer pilus assembly protein TraU | traU |
| Q0L46_RS28945 (Q0L46_28945) | 3443..4060 | + | 618 | WP_301717979 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| Q0L46_RS28950 (Q0L46_28950) | 4057..5931 | + | 1875 | WP_301717980 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| Q0L46_RS28955 (Q0L46_28955) | 5964..6245 | + | 282 | WP_301717981 | hypothetical protein | - |
| Q0L46_RS28960 (Q0L46_28960) | 6235..6468 | + | 234 | WP_301717982 | conjugal transfer protein TrbE | - |
| Q0L46_RS28965 (Q0L46_28965) | 6710..7036 | + | 327 | WP_301717983 | hypothetical protein | - |
| Q0L46_RS28970 (Q0L46_28970) | 7057..7809 | + | 753 | WP_301717984 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| Q0L46_RS28975 (Q0L46_28975) | 7820..8059 | + | 240 | WP_049071719 | type-F conjugative transfer system pilin chaperone TraQ | - |
| Q0L46_RS28980 (Q0L46_28980) | 8031..8603 | + | 573 | WP_301717985 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| Q0L46_RS28985 (Q0L46_28985) | 8596..9024 | + | 429 | WP_301717986 | conjugal transfer protein TrbF | - |
| Q0L46_RS28990 (Q0L46_28990) | 9011..10381 | + | 1371 | WP_301717987 | conjugal transfer pilus assembly protein TraH | traH |
| Q0L46_RS28995 (Q0L46_28995) | 10381..13203 | + | 2823 | WP_301717988 | conjugal transfer mating-pair stabilization protein TraG | traG |
| Q0L46_RS29000 (Q0L46_29000) | 13219..13809 | + | 591 | WP_301717989 | hypothetical protein | - |
| Q0L46_RS29005 (Q0L46_29005) | 13951..14682 | + | 732 | Protein_16 | conjugal transfer complement resistance protein TraT | - |
| Q0L46_RS29010 (Q0L46_29010) | 14877..17198 | + | 2322 | WP_301717990 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 14310 | GenBank | NZ_CP129896 |
| Plasmid name | pRSM9152_1 | Incompatibility group | IncFII |
| Plasmid size | 152910 bp | Coordinate of oriT [Strand] | 145135..145181 [-] |
| Host baterium | Klebsiella michiganensis strain 9152 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |