Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113856 |
| Name | oriT_p1_Eh8322_LBHALD  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain Eh8322_LBHALD |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP129637 (37693..37798 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p1_Eh8322_LBHALD
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACAAGCGGCGTCCTCTCAAAAACGTCGTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACAAGCGGCGTCCTCTCAAAAACGTCGTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 8890 | GenBank | WP_004187323 |
| Name | traI_QY056_RS22280_p1_Eh8322_LBHALD |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 10324 | GenBank | WP_004206885 |
| Name | t4cp2_QY056_RS21995_p1_Eh8322_LBHALD |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 40594..59793
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QY056_RS22260 (QY056_22260) | 35678..35989 | + | 312 | WP_004187333 | hypothetical protein | - |
| QY056_RS22265 (QY056_22265) | 36122..36658 | + | 537 | WP_004187332 | hypothetical protein | - |
| QY056_RS22270 (QY056_22270) | 37160..37555 | - | 396 | WP_019725163 | hypothetical protein | - |
| QY056_RS22275 (QY056_22275) | 37590..38117 | + | 528 | WP_301731758 | plasmid mobilization protein MobA | - |
| QY056_RS22280 (QY056_22280) | 38104..40083 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QY056_RS22285 (QY056_22285) | 40097..40597 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QY056_RS22290 (QY056_22290) | 40594..41373 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QY056_RS22295 (QY056_22295) | 41384..42547 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QY056_RS22300 (QY056_22300) | 42537..42797 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QY056_RS22305 (QY056_22305) | 42822..46169 | + | 3348 | WP_223807062 | LPD7 domain-containing protein | - |
| QY056_RS22310 (QY056_22310) | 46135..46647 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QY056_RS22315 (QY056_22315) | 46648..46860 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QY056_RS22320 (QY056_22320) | 46832..47242 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QY056_RS22325 (QY056_22325) | 47310..48023 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QY056_RS22330 (QY056_22330) | 48032..49183 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QY056_RS22335 (QY056_22335) | 49195..50544 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QY056_RS22340 (QY056_22340) | 50556..51260 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QY056_RS22345 (QY056_22345) | 51284..51814 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QY056_RS22350 (QY056_22350) | 51831..52220 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QY056_RS22355 (QY056_22355) | 52266..52760 | + | 495 | WP_004187480 | hypothetical protein | - |
| QY056_RS22360 (QY056_22360) | 52757..55807 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QY056_RS22365 (QY056_22365) | 55804..57012 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QY056_RS22370 (QY056_22370) | 57009..57605 | + | 597 | WP_015060003 | hypothetical protein | - |
| QY056_RS22375 (QY056_22375) | 57598..59793 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QY056_RS22380 (QY056_22380) | 59795..60448 | + | 654 | WP_015060005 | hypothetical protein | - |
| QY056_RS22385 (QY056_22385) | 60529..60759 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 14291 | GenBank | NZ_CP129637 |
| Plasmid name | p1_Eh8322_LBHALD | Incompatibility group | IncL/M |
| Plasmid size | 61054 bp | Coordinate of oriT [Strand] | 37693..37798 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain Eh8322_LBHALD |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |