Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113821
Name   oriT_p2020S07-155-137k in_silico
Organism   Klebsiella quasipneumoniae strain 2020S07-155
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP129868 (24525..24606 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_p2020S07-155-137k
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8856 GenBank   WP_015632446
Name   traI_QYQ61_RS28810_p2020S07-155-137k insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   10281 GenBank   WP_015632442
Name   trbC_QYQ61_RS28845_p2020S07-155-137k insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32468..54309

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QYQ61_RS28825 (QYQ61_28825) 27527..27934 + 408 WP_015632445 transposase -
QYQ61_RS28830 (QYQ61_28830) 27931..28281 + 351 WP_032414478 IS66 family insertion sequence element accessory protein TnpB -
QYQ61_RS28835 (QYQ61_28835) 28311..29900 + 1590 WP_015632444 IS66 family transposase -
QYQ61_RS28840 (QYQ61_28840) 29923..30228 - 306 WP_015632443 phospholipase D-like domain-containing protein -
QYQ61_RS28845 (QYQ61_28845) 30274..32466 - 2193 WP_015632442 F-type conjugative transfer protein TrbC -
QYQ61_RS28850 (QYQ61_28850) 32468..33595 - 1128 WP_165488786 thioredoxin fold domain-containing protein trbB
QYQ61_RS28855 (QYQ61_28855) 33570..34988 - 1419 WP_015632440 hypothetical protein trbA
QYQ61_RS28860 (QYQ61_28860) 35090..35713 - 624 WP_015632439 plasmid IncI1-type surface exclusion protein ExcA -
QYQ61_RS28865 (QYQ61_28865) 35779..37923 - 2145 WP_040213706 DotA/TraY family protein traY
QYQ61_RS28870 (QYQ61_28870) 37965..38456 - 492 WP_110230419 conjugal transfer protein TraX -
QYQ61_RS28875 (QYQ61_28875) 38543..39739 - 1197 WP_015632436 conjugal transfer protein TraW traW
QYQ61_RS28880 (QYQ61_28880) 39750..42797 - 3048 WP_015632435 conjugal transfer protein traU
QYQ61_RS28885 (QYQ61_28885) 42807..43121 - 315 WP_123677146 hypothetical protein traT
QYQ61_RS28890 (QYQ61_28890) 43414..43815 - 402 WP_015632433 DUF6750 family protein traR
QYQ61_RS28895 (QYQ61_28895) 43869..44402 - 534 WP_015632432 conjugal transfer protein TraQ traQ
QYQ61_RS28900 (QYQ61_28900) 44412..45131 - 720 WP_015632431 conjugal transfer protein TraP traP
QYQ61_RS28905 (QYQ61_28905) 45137..46477 - 1341 WP_015632430 conjugal transfer protein TraO traO
QYQ61_RS28910 (QYQ61_28910) 46481..47584 - 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
QYQ61_RS28915 (QYQ61_28915) 47595..48299 - 705 WP_015632428 DotI/IcmL/TraM family protein traM
QYQ61_RS28920 (QYQ61_28920) 48335..48688 - 354 WP_015632427 hypothetical protein traL
QYQ61_RS28925 (QYQ61_28925) 48722..52045 - 3324 WP_032495745 LPD7 domain-containing protein -
QYQ61_RS28930 (QYQ61_28930) 52063..52329 - 267 WP_032441926 IcmT/TraK family protein traK
QYQ61_RS28935 (QYQ61_28935) 52326..53513 - 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
QYQ61_RS28940 (QYQ61_28940) 53491..54309 - 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
QYQ61_RS28945 (QYQ61_28945) 54306..54773 - 468 WP_015632424 DotD/TraH family lipoprotein -
QYQ61_RS28950 (QYQ61_28950) 54796..55275 - 480 WP_015632423 lytic transglycosylase domain-containing protein -
QYQ61_RS28955 (QYQ61_28955) 55973..56284 - 312 WP_015632421 hypothetical protein -
QYQ61_RS28960 (QYQ61_28960) 56318..56521 - 204 WP_032495743 hypothetical protein -
QYQ61_RS28965 (QYQ61_28965) 56539..57006 - 468 WP_032495742 transcription termination/antitermination NusG family protein -
QYQ61_RS28970 (QYQ61_28970) 57340..57918 + 579 WP_015632419 hypothetical protein -
QYQ61_RS28975 (QYQ61_28975) 57900..58370 + 471 WP_032441927 hypothetical protein -
QYQ61_RS28980 (QYQ61_28980) 58370..58756 + 387 WP_015632417 thioesterase family protein -

Host bacterium

ID   14256 GenBank   NZ_CP129868
Plasmid name   p2020S07-155-137k Incompatibility group   IncFII
Plasmid size   137053 bp Coordinate of oriT [Strand]   24525..24606 [+]
Host baterium   Klebsiella quasipneumoniae strain 2020S07-155

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -