Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 113757
• Name: oriT_KMGR2-43|unnamed4
• Organism: Lactococcus lactis subsp. lactis strain KMGR2-43
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP129296 (28877..28914 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 1..7, 19..25 (ACACCAC..GTGGTGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_KMGR2-43|unnamed4
ACACCACACATTTTTGGGGTGGTGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_QYH60_RS13855_KMGR2-43|unnamed4

• ID: 8804
• GenBank: WP_301536283
• Name: mobQ_QYH60_RS13855_KMGR2-43|unnamed4
• UniProt ID: _
• Length: 680 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 680 a.a.
• Molecular weight: 79250.35 Da
• Isoelectric Point: 6.5251

>WP_301536283.1 MobQ family relaxase [Lactococcus lactis]
MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYDRSVMPESFILLPENAPEWAKDRQKLWN
EVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKTFVDKGMVADVAIHRDHDENPHAHVMLTNR
PFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLVDWDKVGKVEEWRKAWADHVNSVFQEKNIDE
RISEKTLEAQGINDIATQHVGVTGNRDERAEFNKLVLENRQHKAELENLDEKINNELKIKQLKNFYSFNE
KKVIAELSKELHTFIDLEHLEEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSQQEFSLDNANRLIDKVVD
RSIKAMYPTVDSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS
KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEVRKIVEQQYSV
VLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPILFSTKEHNQGLSYLAGRIRL
EDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEATNRNDKQSYKNEFYRDKNMENYQSVAYSDTS
PAEYMNRLFSGVLISVLYNNENQHKKGLEEVEWDMERKHREHTKTGGLSR

Protein domains

Predicted by InterproScan.

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-383)

• MobA/MobL family (MobA_MobL) (17-237)

Protein structure

No available structure.

T4CP

t4cp2_QYH60_RS13715_KMGR2-43|unnamed4

• ID: 10209
• GenBank: WP_301536286
• Name: t4cp2_QYH60_RS13715_KMGR2-43|unnamed4
• UniProt ID: _
• Length: 530 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 530 a.a.
• Molecular weight: 59688.40 Da
• Isoelectric Point: 4.8471

>WP_301536286.1 VirD4-like conjugal transfer protein, CD1115 family [Lactococcus lactis]
MNGTILGVLDNKIIYQDNTTKPNRNVMVIGGSGSYKTQSVVITNLFNETKNSIVVTDPKGELYEKTAGIK
LAQGYEVHVVNFANMAHSDRYNPFDYIERDIQAESVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMK
ERSPEQRNLAGVINVLQTFDSEPINKDENSDLDNLFLALKITHPARIAYELGFKKAKGDMKASIISSLLA
TISKFTDEEVSNFTSISDFHLQDIGRKKIVLYVIIPVMDNTYESFINLFFSQMFDELYKLASSNGAKLPQ
EVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQSLYGKEKAESILGNHAVKICLNASNEA
TAKYFSELLGKSTVKVETGSESTSHSKETSTSKSDSYSYTSRQLMTPDEIIRMPDTQSLLIFTNQKPIKA
TKAFQFKLFPDADSKVKLEQNKYVGITSKSQLEKYNDLSVKWEKKLQSLKNITVTEEEEKDLQDNIDSDL
EAMNNENSEVDNSTTETDKIAETETKEAQAEEKNFDQVVL

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (25-452)

Protein structure

No available structure.

Host bacterium

• ID: 14192
• GenBank: NZ_CP129296
• Plasmid name: KMGR2-43|unnamed4
• Incompatibility group: -
• Plasmid size: 34541 bp
• Coordinate of oriT [Strand]: 28877..28914 [-]
• Host baterium: Lactococcus lactis subsp. lactis strain KMGR2-43

Cargo genes

• Drug resistance gene: tet(S)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -