Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113752
Name   oriT_p1MP4m4 in_silico
Organism   Enterobacter asburiae strain MP4m4
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP129498 (266677..266781 [+], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      56..61, 74..79  (TGGAAT..ATTCCA)
 46..51, 56..61  (ATTCCA..TGGAAT)
 1..6, 8..13  (AATTTG..CAAATT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT_p1MP4m4
AATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   10207 GenBank   WP_004883083
Name   t4cp2_QOM25_RS25190_p1MP4m4 insolico UniProt ID   _
Length   671 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 671 a.a.        Molecular weight: 73455.71 Da        Isoelectric Point: 6.5506

>WP_004883083.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MQGTNVLFGQIAVVFGIVIAGVWGATQWTAAALGYQVRLGSPWFDFLGTPIYLPWKLFEWWFFFDAYAPR
VFDTGGAIAGGSGLLAVVVAVCMSIWRSRQSRLVTTYGSARWASTEDIRKAGLTQPIGVFLGQHDGKYLR
HEGPEHILTFAPTRSGKGVGLVVPTLLSWPASAVVHDIKGENWQITAGWRSRYSHCLLFNPTDAKSAAYN
PLLEVRRGAHEVRDVQNIADILVDPEGALEKRNHWEKTSHALLVGAILHVLYAGEDKTLRGVANFLSDPA
SPFELTLHRMMTTQHLVNVEGGGPHPVVASAAREVLNKSDNERSGVLSTAMSFLGLYRDPTVAEVTSRCD
WRIADLIASEHPVSLYLVVPPSDISRTKPLIRLILNQIGRRLTESLDGSDGIERRHKLLLMLDEFPALGR
LDFFETALAFMAGYGIRSFLIAQSLNQIDKAYGQNHSILDNCHVRVTFATNDERTAKRISETLGTATELR
AQRNYAGHRLAPWLGHLMVSRQETARPLLTPGEVMQLPTEEAVVMVSSVAPIKAKKLRYYADANFKNRVL
PPPVLAAGQYADAPPARPDDWSGLSIPAVSTVRAAGLSGDGTGTADDGGPRRQPELSEVSEYSPEPLLAG
HDLTLLDDDDDLPLPLPGQLDPAMQRTARLASLDPNDGIDL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(112-562)

  Protein structure



No available structure.



ID   10208 GenBank   WP_004883099
Name   trbE_QOM25_RS25215_p1MP4m4 insolico UniProt ID   _
Length   816 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 816 a.a.        Molecular weight: 89531.64 Da        Isoelectric Point: 5.2838

>WP_004883099.1 MULTISPECIES: conjugal transfer protein TrbE [Pseudomonadota]
MLNLAEYRQRPALLADWLPWAGLIAPGVVLNKDGSFQRTARFRGPDLDSATQGELIATTARLNNALRRLG
SGWALFVEAERQSAADYPHSDFPEPLSWLVDEERRAAFEESGNHYESSYHLTLAYLPPEESRARAAKLLY
EHAPGDGVDWRGRLDAFLAETDRVFDLLDGVMPEIVWLDDAATLTYLHATVSTRRYRLAVPEVPFHLDAL
LADSALVGGLAPMLGDQHLRVVSVRGFPTSTWPGLLDDLNRLGFAYRWSTRFLCLDKAEAEKELGRLRRQ
WFAKRKNVIALLRETIFQQESPLVDTDASNKAADADAALQELGSDQVAFGYLTATVTVFDDDPAVADEKL
RMVERVIQGRGFVTIPETLNAVDAWLSSIPGNAYANVRQPIVSTLNLAHMMPVSAVWAGPEKNAHLDGPP
LIVTRTDGATPFRLVTHIGDVGHTLVAGPTGMGKSVLLAMLALQFRRYGGSRIFAFDMGRSMRATVLGLG
GEHYDLGADGAIAFQPLARIDSEGYRTWAAEWIEGRLLHEGVTVGPDEKAAIWSALGSLAGAPVEQRTMT
GLSVLLQSNALRQALSPYVLGGAHGKLLDADRDRLGSGDVQGFEMEELMHSPAAVQAVLRYLFARFDARF
DGAPTLLILDEAWLFLDEPSFAARIRQWLKTLRKKNVSVIFATQSLADIKDSTIAPAIIESCASRIFLPN
PQATEPQIRTVYEGFGLNSRQIEIVATAQPKRDYYYQSRLGNRLFDLDLGPAALAFSGASTPQDQRDIDR
VLTQAGAPGFAGAWLRHRGLDWAADLLPSSPSAASFLATQPLEISP

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(571-726)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(187-388)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 144059..153677

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOM25_RS25180 (QOM25_25180) 140103..141047 + 945 WP_004883081 LysR family transcriptional regulator -
QOM25_RS25185 (QOM25_25185) 141044..141319 + 276 WP_004883082 EexN family lipoprotein -
QOM25_RS25190 (QOM25_25190) 141586..143601 + 2016 WP_004883083 conjugal transfer protein TraG -
QOM25_RS25195 (QOM25_25195) 143598..144062 + 465 WP_004883086 ribbon-helix-helix protein, CopG family -
QOM25_RS25200 (QOM25_25200) 144059..145126 + 1068 WP_004883088 P-type conjugative transfer ATPase TrbB virB11
QOM25_RS25205 (QOM25_25205) 145123..145506 + 384 WP_004883090 TrbC/VirB2 family protein virB2
QOM25_RS25210 (QOM25_25210) 145503..145775 + 273 WP_004883097 VirB3 family type IV secretion system protein virB3
QOM25_RS25215 (QOM25_25215) 145787..148237 + 2451 WP_004883099 conjugal transfer protein TrbE virb4
QOM25_RS25220 (QOM25_25220) 148234..148971 + 738 WP_004883101 P-type conjugative transfer protein TrbJ virB5
QOM25_RS25225 (QOM25_25225) 148984..149310 + 327 WP_004883103 hypothetical protein -
QOM25_RS25230 (QOM25_25230) 149307..150671 + 1365 WP_004883106 P-type conjugative transfer protein TrbL virB6
QOM25_RS25235 (QOM25_25235) 150701..151405 + 705 WP_004883108 conjugal transfer protein TrbF virB8
QOM25_RS25240 (QOM25_25240) 151402..152394 + 993 WP_004883112 P-type conjugative transfer protein TrbG virB9
QOM25_RS25245 (QOM25_25245) 152397..153677 + 1281 WP_004883119 TrbI/VirB10 family protein virB10
QOM25_RS25250 (QOM25_25250) 153674..153916 + 243 WP_004883121 DUF2274 domain-containing protein -
QOM25_RS25255 (QOM25_25255) 154209..154604 - 396 WP_223203170 DNA-binding protein -
QOM25_RS25260 (QOM25_25260) 154594..154866 - 273 WP_022652138 hypothetical protein -
QOM25_RS25265 (QOM25_25265) 155180..155506 - 327 WP_015063087 hypothetical protein -
QOM25_RS25270 (QOM25_25270) 155695..156210 + 516 WP_015063086 hypothetical protein -
QOM25_RS25275 (QOM25_25275) 156798..157679 - 882 WP_040115182 RepB family plasmid replication initiator protein -

Host bacterium

ID   14187 GenBank   NZ_CP129498
Plasmid name   p1MP4m4 Incompatibility group   IncA/C2
Plasmid size   267622 bp Coordinate of oriT [Strand]   266677..266781 [+]
Host baterium   Enterobacter asburiae strain MP4m4

Cargo genes

Drug resistance gene   formA
Virulence gene   -
Metal resistance gene   merE, merD, merB, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -