Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 113728 |
| Name | oriT_16A242|unnamed2  |
| Organism | Salmonella enterica subsp. enterica strain 16A242 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP020924 (39600..39648 [-], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_16A242|unnamed2
CGATTGTAACATACCCGGAACGGGTTTGTGTACAATCGGTCTCGTGCCT
CGATTGTAACATACCCGGAACGGGTTTGTGTACAATCGGTCTCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10187 | GenBank | WP_000726942 |
| Name | t4cp2_B6N26_RS25910_16A242|unnamed2 |
UniProt ID | _ |
| Length | 655 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 655 a.a. Molecular weight: 74097.02 Da Isoelectric Point: 8.2345
>WP_000726942.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MKKKIILVVFLLLILVVCLIGGNYLGGYAALKYSGLNIDMLQWNTFNNVITQFSGKPEYKKLVAITWAGF
AAPCAIFIGFVVIVIVGLMPKKVIYGNARLATDMDLAKSTFFPTDKELKEARLKNSKPYCFPPILIGKQF
KGRFKNKYIYFFGQQFLILYAPTRSGKGVGIVIPNCVNYPDSMVVLDIKLENWFLSAGYRQNVLGQECFL
FAPAGYAENQQEAKKGNIRSHRWNPLDCVNRSDIQRSGDLEKIAAMLIPASDDPIWSDSARKLFCGLALY
LLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQKEYLSDETKSLFREFMAAP
EKTQGSIITNFSSPLSIFKNPITAAATNASDFDVRMVRKKPMSIYLGLTPDALITHSRLVNLFFSMLVNE
NTKELPEQNPELKYQCLVLLDEFTSMGKSEIIEKGVGFTAGFNLRFVIILQNEGQGKKDDMYGTHGWETF
VENSAVVLYYPPKAKNELAKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSRNHEIVSRAVLLPEEIVELR
DYKNKMGNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMPVLFDEEMRNQILEQAKIYS
DDISMFSDVMTKPDLETHDLEDVSE
MKKKIILVVFLLLILVVCLIGGNYLGGYAALKYSGLNIDMLQWNTFNNVITQFSGKPEYKKLVAITWAGF
AAPCAIFIGFVVIVIVGLMPKKVIYGNARLATDMDLAKSTFFPTDKELKEARLKNSKPYCFPPILIGKQF
KGRFKNKYIYFFGQQFLILYAPTRSGKGVGIVIPNCVNYPDSMVVLDIKLENWFLSAGYRQNVLGQECFL
FAPAGYAENQQEAKKGNIRSHRWNPLDCVNRSDIQRSGDLEKIAAMLIPASDDPIWSDSARKLFCGLALY
LLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQKEYLSDETKSLFREFMAAP
EKTQGSIITNFSSPLSIFKNPITAAATNASDFDVRMVRKKPMSIYLGLTPDALITHSRLVNLFFSMLVNE
NTKELPEQNPELKYQCLVLLDEFTSMGKSEIIEKGVGFTAGFNLRFVIILQNEGQGKKDDMYGTHGWETF
VENSAVVLYYPPKAKNELAKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSRNHEIVSRAVLLPEEIVELR
DYKNKMGNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMPVLFDEEMRNQILEQAKIYS
DDISMFSDVMTKPDLETHDLEDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 10188 | GenBank | WP_253746204 |
| Name | t4cp2_B6N26_RS26880_16A242|unnamed2 |
UniProt ID | _ |
| Length | 394 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 394 a.a. Molecular weight: 44714.47 Da Isoelectric Point: 5.1547
>WP_253746204.1 type IV secretory system conjugative DNA transfer family protein [Salmonella enterica]
MILFGLILHGSSFCGLALYLLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQ
KEYLSDETKSLFREFMAAPEKTQGSIITNFSSPLSIFKNPITAAATNASDFDVRMVRKKPMSIYLGLTPD
ALITHSRLVNLFFSMLVNENTKELPEQNPELKYQCLVLLDEFTSMGKSEIIEKGVGFTAGFNLRFVIILQ
NEGQGKKDDMYGTHGWETFVENSAVVLYYPPKAKNELAKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSR
NHEIVSRAVLLPEEIVELRDYKNKMGNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMP
VLFDEEMRNQILEQAKIYSDDISMFSDVMTKPDLETHDLEDVSE
MILFGLILHGSSFCGLALYLLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQ
KEYLSDETKSLFREFMAAPEKTQGSIITNFSSPLSIFKNPITAAATNASDFDVRMVRKKPMSIYLGLTPD
ALITHSRLVNLFFSMLVNENTKELPEQNPELKYQCLVLLDEFTSMGKSEIIEKGVGFTAGFNLRFVIILQ
NEGQGKKDDMYGTHGWETFVENSAVVLYYPPKAKNELAKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSR
NHEIVSRAVLLPEEIVELRDYKNKMGNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMP
VLFDEEMRNQILEQAKIYSDDISMFSDVMTKPDLETHDLEDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 18344..27117
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B6N26_RS25900 (B6N26_04885) | 15295..15882 | - | 588 | WP_000280687 | DUF6012 family protein | - |
| B6N26_RS25905 (B6N26_04886) | 16005..16310 | - | 306 | WP_001223992 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| B6N26_RS25910 (B6N26_04887) | 16326..18293 | - | 1968 | WP_000726942 | type IV secretory system conjugative DNA transfer family protein | - |
| B6N26_RS25915 (B6N26_04888) | 18344..19363 | - | 1020 | WP_001287983 | P-type DNA transfer ATPase VirB11 | virB11 |
| B6N26_RS25920 (B6N26_04889) | 19363..20505 | - | 1143 | WP_087526206 | TrbI/VirB10 family protein | virB10 |
| B6N26_RS25925 (B6N26_04890) | 20495..21274 | - | 780 | WP_000592647 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| B6N26_RS25930 (B6N26_04891) | 21271..22008 | - | 738 | WP_000364523 | virB8 family protein | virB8 |
| B6N26_RS25935 (B6N26_04893) | 22139..24511 | - | 2373 | WP_053293934 | VirB4 family type IV secretion system protein | virb4 |
| B6N26_RS25940 (B6N26_04894) | 24514..24834 | - | 321 | WP_001000143 | VirB3 family type IV secretion system protein | virB3 |
| B6N26_RS25945 (B6N26_04895) | 24892..25185 | - | 294 | WP_001031682 | TrbC/VirB2 family protein | virB2 |
| B6N26_RS25950 (B6N26_04896) | 25178..25789 | - | 612 | WP_000603936 | lytic transglycosylase domain-containing protein | virB1 |
| B6N26_RS25955 (B6N26_04898) | 26122..27117 | + | 996 | WP_001103652 | type IV secretion system protein | virB6 |
| B6N26_RS25960 (B6N26_04899) | 27119..27760 | + | 642 | WP_000844300 | type IV secretion system protein | - |
| B6N26_RS25965 (B6N26_04900) | 27773..28006 | + | 234 | WP_001056371 | EexN family lipoprotein | - |
| B6N26_RS26560 (B6N26_04901) | 28047..28334 | - | 288 | WP_000332248 | helix-turn-helix transcriptional regulator | - |
| B6N26_RS25975 (B6N26_04902) | 28533..28871 | + | 339 | WP_000612441 | helix-turn-helix domain-containing protein | - |
| B6N26_RS25980 (B6N26_04903) | 29245..30249 | - | 1005 | WP_001208090 | replication initiation protein | - |
Host bacterium
| ID | 14163 | GenBank | NZ_CP020924 |
| Plasmid name | 16A242|unnamed2 | Incompatibility group | IncX4 |
| Plasmid size | 65419 bp | Coordinate of oriT [Strand] | 39600..39648 [-] |
| Host baterium | Salmonella enterica subsp. enterica strain 16A242 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |