Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   113679
Name   oriT_Y7-3|unnamed2 in_silico
Organism   Klebsiella quasipneumoniae strain Y7-3
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP099836 (96435..96483 [-], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site  
 
 GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_Y7-3|unnamed2
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 95877..119923

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NDO72_RS26855 (NDO72_26855) 90979..91191 + 213 WP_013023815 hypothetical protein -
NDO72_RS26860 (NDO72_26860) 91202..91426 + 225 WP_004152719 hypothetical protein -
NDO72_RS26865 (NDO72_26865) 91507..91827 + 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
NDO72_RS26870 (NDO72_26870) 91817..92095 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
NDO72_RS26875 (NDO72_26875) 92096..92509 + 414 WP_013023817 type II toxin-antitoxin system HigA family antitoxin -
NDO72_RS26880 (NDO72_26880) 92645..93853 - 1209 WP_015344990 IS256 family transposase -
NDO72_RS26885 (NDO72_26885) 94661..95482 + 822 WP_004152492 DUF932 domain-containing protein -
NDO72_RS26890 (NDO72_26890) 95515..95844 + 330 WP_011977736 DUF5983 family protein -
NDO72_RS26895 (NDO72_26895) 95877..96362 - 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
NDO72_RS26900 (NDO72_26900) 96794..97186 + 393 WP_004194114 conjugal transfer relaxosome DNA-binding protein TraM -
NDO72_RS26905 (NDO72_26905) 97416..98117 + 702 WP_004194113 hypothetical protein -
NDO72_RS26910 (NDO72_26910) 98203..98403 + 201 WP_004194116 TraY domain-containing protein -
NDO72_RS26915 (NDO72_26915) 98472..98840 + 369 WP_004194426 type IV conjugative transfer system pilin TraA -
NDO72_RS26920 (NDO72_26920) 98854..99159 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
NDO72_RS26925 (NDO72_26925) 99179..99745 + 567 WP_004144423 type IV conjugative transfer system protein TraE traE
NDO72_RS26930 (NDO72_26930) 99732..100472 + 741 WP_013023821 type-F conjugative transfer system secretin TraK traK
NDO72_RS26935 (NDO72_26935) 100472..101896 + 1425 WP_004194260 F-type conjugal transfer pilus assembly protein TraB traB
NDO72_RS26940 (NDO72_26940) 102010..102594 + 585 WP_013023822 type IV conjugative transfer system lipoprotein TraV traV
NDO72_RS26945 (NDO72_26945) 102726..103136 + 411 WP_009309869 hypothetical protein -
NDO72_RS26950 (NDO72_26950) 103242..103460 + 219 WP_015344987 hypothetical protein -
NDO72_RS26955 (NDO72_26955) 103461..103772 + 312 WP_015344986 hypothetical protein -
NDO72_RS26960 (NDO72_26960) 103839..104243 + 405 WP_004197817 hypothetical protein -
NDO72_RS26965 (NDO72_26965) 104619..105017 + 399 WP_013609531 hypothetical protein -
NDO72_RS26970 (NDO72_26970) 105089..107728 + 2640 WP_013023824 type IV secretion system protein TraC virb4
NDO72_RS26975 (NDO72_26975) 107728..108117 + 390 WP_004197815 type-F conjugative transfer system protein TrbI -
NDO72_RS26980 (NDO72_26980) 108117..108743 + 627 WP_009309871 type-F conjugative transfer system protein TraW traW
NDO72_RS26985 (NDO72_26985) 108785..109174 + 390 WP_004194992 hypothetical protein -
NDO72_RS26990 (NDO72_26990) 109171..110160 + 990 WP_009309872 conjugal transfer pilus assembly protein TraU traU
NDO72_RS26995 (NDO72_26995) 110173..110811 + 639 WP_011977786 type-F conjugative transfer system pilin assembly protein TrbC trbC
NDO72_RS27000 (NDO72_27000) 110870..112825 + 1956 WP_013023827 type-F conjugative transfer system mating-pair stabilization protein TraN traN
NDO72_RS27005 (NDO72_27005) 112857..113111 + 255 WP_004152674 conjugal transfer protein TrbE -
NDO72_RS27010 (NDO72_27010) 113089..113337 + 249 WP_004152675 hypothetical protein -
NDO72_RS27015 (NDO72_27015) 113350..113676 + 327 WP_004152676 hypothetical protein -
NDO72_RS27020 (NDO72_27020) 113697..114449 + 753 WP_048337413 type-F conjugative transfer system pilin assembly protein TraF traF
NDO72_RS27025 (NDO72_27025) 114460..114699 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
NDO72_RS27030 (NDO72_27030) 114671..115228 + 558 WP_013214031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
NDO72_RS27035 (NDO72_27035) 115274..115717 + 444 WP_013023828 F-type conjugal transfer protein TrbF -
NDO72_RS27040 (NDO72_27040) 115695..117074 + 1380 WP_014343487 conjugal transfer pilus assembly protein TraH traH
NDO72_RS27045 (NDO72_27045) 117074..119923 + 2850 WP_013609534 conjugal transfer mating-pair stabilization protein TraG traG
NDO72_RS27050 (NDO72_27050) 119926..120459 + 534 WP_014343486 conjugal transfer protein TraS -
NDO72_RS27055 (NDO72_27055) 120645..121376 + 732 WP_013023830 conjugal transfer complement resistance protein TraT -
NDO72_RS27060 (NDO72_27060) 121569..122258 + 690 WP_013023831 hypothetical protein -


Host bacterium


ID   14114 GenBank   NZ_CP099836
Plasmid name   Y7-3|unnamed2 Incompatibility group   IncFII
Plasmid size   123534 bp Coordinate of oriT [Strand]   96435..96483 [-]
Host baterium   Klebsiella quasipneumoniae strain Y7-3

Cargo genes


Drug resistance gene   qnrS1, blaCTX-M-3, blaTEM-1B, floR, tet(A), mph(A), sul1, qacE, aadA16, dfrA27, ARR-3, aac(6')-Ib-cr
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9