Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   113651
Name   oriT_pOXA_17620 in_silico
Organism   Enterobacter hormaechei strain 17620
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ127402 (40236..40341 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_pOXA_17620
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   8745 GenBank   WP_004187323
Name   Relaxase_QN257_RS00315_pOXA_17620 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   10124 GenBank   WP_004206885
Name   t4cp2_QN257_RS00015_pOXA_17620 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 43137..62426

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN257_RS00295 38221..38532 + 312 WP_004187333 hypothetical protein -
QN257_RS00300 38665..39201 + 537 WP_004187332 hypothetical protein -
QN257_RS00305 39703..40098 - 396 WP_019725163 hypothetical protein -
QN257_RS00310 40097..40660 + 564 WP_020277892 plasmid mobilization protein MobA -
QN257_RS00315 40647..42626 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
QN257_RS00320 42640..43140 + 501 WP_004187320 DotD/TraH family lipoprotein -
QN257_RS00325 43137..43916 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
QN257_RS00330 43927..45090 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
QN257_RS00335 45080..45340 + 261 WP_004187310 IcmT/TraK family protein traK
QN257_RS00340 45365..48802 + 3438 WP_015586048 LPD7 domain-containing protein -
QN257_RS00345 48768..49280 + 513 WP_011091071 hypothetical protein traL
QN257_RS00350 49281..49478 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
QN257_RS00355 49465..49875 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
QN257_RS00360 49874..50656 + 783 WP_015058941 DotI/IcmL family type IV secretion protein traM
QN257_RS00365 50665..51816 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
QN257_RS00370 51828..53177 + 1350 WP_004187474 conjugal transfer protein TraO traO
QN257_RS00375 53189..53893 + 705 WP_015060002 conjugal transfer protein TraP traP
QN257_RS00380 53917..54447 + 531 WP_004187478 conjugal transfer protein TraQ traQ
QN257_RS00385 54464..54853 + 390 WP_004187479 DUF6750 family protein traR
QN257_RS00390 54899..55393 + 495 WP_004187480 hypothetical protein -
QN257_RS00395 55390..58440 + 3051 WP_004187482 hypothetical protein traU
QN257_RS00400 58437..59645 + 1209 WP_011091082 conjugal transfer protein TraW traW
QN257_RS00405 59642..60238 + 597 WP_032419542 hypothetical protein -
QN257_RS00410 60231..62426 + 2196 WP_015062834 DotA/TraY family protein traY
QN257_RS00415 62428..63081 + 654 WP_015060005 hypothetical protein -
QN257_RS00420 63150..63392 + 243 WP_023893611 IncL/M type plasmid replication protein RepC -

Host bacterium

ID   14086 GenBank   NZ_OQ127402
Plasmid name   pOXA_17620 Incompatibility group   IncL/M
Plasmid size   63675 bp Coordinate of oriT [Strand]   40236..40341 [+]
Host baterium   Enterobacter hormaechei strain 17620

Cargo genes

Drug resistance gene   blaOXA-48
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -